Objective To study the concentration of adhesion-related cytokines,transforming growth factor (TGF) β1,platelet-derived growth factor (PDGF) BB,b-fibroblast growth factor (bFGF) in intrauterine fluid and their roles in the intrauterine-adhesion reformation.Methods From August 2009 to February 2010,18 patients with moderate or severe intrauterine adhesions underwent transcervical resection of intrauterine adhesion as study group,in the mean time,20 patients with incomplete septate uterus underwent transcervical resection of septum as control group.The charge of each patient's intrauterine fluid was collected at the time of 3,6,9,12,24,48 and 72 hours,respectively,then the concentration of the three cytokines was measured by enzyme-linked immunosorbent assay(ELISA).Results The concentration of the expression of TGF-β1 in the intrauterine fluid at 3,6,9,12 hours after operation in study group was (3.6±0.9),(10.4±1.1),(7.6±1.2),(7.2±1.3) ng/ml,respectively; PDGF-BB was(2.6±0.6),(3.5 ±0.5),(5.4±1.0),(5.7 ±0.8) ng/ml,respectively; bFGF was(16.9±1.3),(95.8±17.8),(330.9 ± 70.5),(1303.3 ± 117.4) ng/ml,respectively.The expression of TGF-β1 in control group was (3.0 ± 0.6),(7.5 ± 0.6),(5.4 ± 0.6),(4.6 ± 0.8) ng/ml,respectively ; PDGF-BB was (2.5 ± 0.4),(2.6±0.5),(4.7±0.6),(4.4±0.4) ng/ml,respectively; bFGF was (19.1 ±2.4),(82.9±21.8),(249.0 ±54.2),(775.6 ± 178.8) ng/ml,respectively.The concentration of TGF-β1,PDGF-BB and bFGF reached the peak during the time of 3-24 hours,6-48 hours,6-72 hours after surgery,respectively.The concentration of TGF-β1,PDGF-BB and bFGF in study group were significantly higher than that in control group (P ＜0.05).Conclusions Concentration of TGF-β1,PDGF-BB and bFGF was positively associated with area of transcervical resection of intrauterine adhesion,those cytokines might be involved in reformatin of intrauterine-adhesion.

Cerebral Infarction ; drug therapy ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Humans ; Phytotherapy ; methods

Aim To study the effects and mechanism of Guanxinkang capsules on myocardial injury induced by isoproterenol in rats.Methods 30 male Wistar rats were randomly divided into 3 groups:control group,acute myocardial ischemia group and Guanxinkang capsules group.Rats were given injections of ISO subcutaneously.Electrocardiography were performed,serum and myocardium creatine kinase(CK),creatine kinase MBmass(CK-MB),lactate dehydrogenase(LDH) and nitric oxide(NO) were detected,and the levels of NO and endothelins(ET) in myocardium were measured as well.Results The rising degree of ST-T obviously decreased in Guanxinkang capsules group,and the levels of serum CK,CK-MB and LDH decreased in Guanxinkang capsules group,compared with those of acute myocardial ischemia group(P

Objective To observe the hemodynamic change of small artery in patients with type 2 diabetes. Methods We measured peak systolic velocity (PSV), end-diastolic velocity (EDV), pulse index (PI), resistance index (RI) of ocular artery (OA), central retinal artery (CRA), finger artery, dorsal pedis artery and interlobar artery of patients with early-stage type 2 diabetes. Results The decrease in PSV and EDV values occurred early in CRA of type 2 diabetics while RI and PI values increased. Conclusion The hemodynamic change of CRA occurs earliest among general small arteries of type 2 diabetic patients.

Using bioactive compounds 7-hydroxy flavone, salicylaldehyde, cinnamic acid and 4-amino-5- mercapto-1,2,4-triazoles as starting materials, three new types of flavone derivatives containing 1,2,4-triazole structure were synthesized via different step reactions. These new compounds were characterized by 1IHNMR, ESI-MS, IR and elemental analysis. Their scavenging effects on the superoxide radical (O2·-), hydroxyl radical (·OH), DPPH · radical and their total reduction activities were tested. The results showed that all of the compounds possessed some antioxidative activity at the concentration of 0.5 mg · mL(-1), but the scavenging ability of the target compounds was lower than that of the standard compound Vc.
Cinnamates ; chemistry ; Flavones ; chemical synthesis ; chemistry ; Flavonoids ; chemistry ; Free Radical Scavengers ; chemical synthesis ; chemistry ; Triazoles ; chemistry

Child, Preschool ; Female ; Humans ; Infant ; Male ; Mycophenolic Acid ; adverse effects ; analogs & derivatives ; therapeutic use ; Nephrotic Syndrome ; drug therapy ; Treatment Outcome

Objective To explore the association between clinical pathological characteristics and the expressions of epidermal growth factor receptor (EGFR) and protein kinase B (AKT) in gastric carcinomas.Methods The expressions of EGFR and AKT were measured with immunohistochemical method in the cancer tissues and cancer-adjacent normal tissues from 153 cases of patients with gastric cancer.The association between clinical pathological characteristics and their expressions were analyzed.Results The expressions of AKT and EGFR in gastric cancer tissues had no relationship with gender,age,pathological type,and the degree of differentiation (P ＞ 0.05).A positive correlation was existed between the EGFR and TNM stages (x2 =5.43,P ＜0.05).The AKT was positively related to the size,T stage,and TNM stage of the tumor,respectively (x2 =4.73,4.95,5.32,P ＜0.05 orP ＜0.01).The levels of AKT (x2=4.83,4.75,P ＜0.05) and EGFR(x2 =4.67,4.58,P ＜0.05) in the gastric cancer tissues with lymph node and/or distant metastasis were significantly higher than the gastric cancer tissues without metastasis,respectively.Conclusions The over-expressions of AKT and EGFR would benefit the diagnosis and stages of a gastric cancer and the determination of its metastasis.

Objective To analyze the diagnostic value of serum procalcitonin ( PCT ) for early postoperative bacterial infection after pediatric living donor liver transplantation.Methods A retrospective study was conducted in pediatric patients after living donor liver transplantation recipients admitted to department of critical care medicine of Beijing Friendship Hospital affiliated to Capital Medical University during June 2013 to October 2015.According to the clinical data , all pediatric patients were divided into infection group(n=60) and non-infection group (n=100).Primary disease, PCT post operation day 1 to day 5 for non-infection group and day 1 to day 9 for infection group , temperature , white blood cell , cold ischemia time, warm ischemia time, operation time, volume of blood loss during operation were recorded.All parameters above were compared between groups.Receiver operating characteristic ( ROC) curve was plotted, and the diagnostic value of PCT was evaluated.Results PCT of both groups were elevated after liver transplantation , there was a markedly resolution in non-infection group within 48 to 72 hours.PCT of pediatric patients with bacterial infection was significantly higher than that of non-infected patients , and the difference was of greatly significant (4.62 ±1.39) ng/ml vs (0.85 ±0.19) ng/ml,t=26.56,P=0.00.ROC curve showed that the peak level of PCT might be valuable in the diagnosis of bacterial infection ( AUC=0.985).There was no significant difference of cold ischemia time [(109.92 ±19.22) min vs (108.04 ± 13.20) min, t=1.05, P=0.29], warm ischemia time[(1.49 ±0.17) min vs (1.52 ±0.12) min, t=1.08, P=0.28], operation time[(8.01 ±0.77)vs (8.00 ±1.05) h, t=0.06, P=0.94], WBC[(8.95 ±1.69) ×109/L vs (8.98 ±2.00) ×109/L,t=-0.08, P=0.93]and body temperature[(37.5 ±0.7) vs (37.5 ±0.8) ℃,t=-0.05, P=0.96] on the first day after surgery between infection and non-infection groups.Amount of bleeding in infection group was higher than that of non infection group [ ( 650.87 ± 90.36) ml vs (240.29 ±67.67) ml, t=32.33, P=0.00], there was longer length of ICU stay in the infection group[(11.01 ±1.81)d vs (6.03 ±1.65)d, t=17.78, P=0.00].Conclusion Peak PCT level was a valuable indicator for early postoperative bacterial infection after pediatric living donor liver transplantation.

Objective To review our clinical experience on 12 patients with retroperitoneal infection who were treated with retroperitoneal laparoscopic debridement.Method This retrospective study included 12 patients with retroperitoneal infection who were treated with retroperitoneal laparoscopic dehridement and drainage.Results All the 12 patients recovered well and were finally discharged home.Conclusions Retroperitoneal laparoscopic debridement and drainage for retroperitoneal infection is a mini-invasive procedure.It was found to be safe,produced minimal bleeding and resulted in rapid postoperative recovery.It can be used as the first choice treatment in properly selected patients.