Objective To study the concentration of adhesion-related cytokines,transforming growth factor (TGF) β1,platelet-derived growth factor (PDGF) BB,b-fibroblast growth factor (bFGF) in intrauterine fluid and their roles in the intrauterine-adhesion reformation.Methods From August 2009 to February 2010,18 patients with moderate or severe intrauterine adhesions underwent transcervical resection of intrauterine adhesion as study group,in the mean time,20 patients with incomplete septate uterus underwent transcervical resection of septum as control group.The charge of each patient's intrauterine fluid was collected at the time of 3,6,9,12,24,48 and 72 hours,respectively,then the concentration of the three cytokines was measured by enzyme-linked immunosorbent assay(ELISA).Results The concentration of the expression of TGF-β1 in the intrauterine fluid at 3,6,9,12 hours after operation in study group was (3.6±0.9),(10.4±1.1),(7.6±1.2),(7.2±1.3) ng/ml,respectively; PDGF-BB was(2.6±0.6),(3.5 ±0.5),(5.4±1.0),(5.7 ±0.8) ng/ml,respectively; bFGF was(16.9±1.3),(95.8±17.8),(330.9 ± 70.5),(1303.3 ± 117.4) ng/ml,respectively.The expression of TGF-β1 in control group was (3.0 ± 0.6),(7.5 ± 0.6),(5.4 ± 0.6),(4.6 ± 0.8) ng/ml,respectively ; PDGF-BB was (2.5 ± 0.4),(2.6±0.5),(4.7±0.6),(4.4±0.4) ng/ml,respectively; bFGF was (19.1 ±2.4),(82.9±21.8),(249.0 ±54.2),(775.6 ± 178.8) ng/ml,respectively.The concentration of TGF-β1,PDGF-BB and bFGF reached the peak during the time of 3-24 hours,6-48 hours,6-72 hours after surgery,respectively.The concentration of TGF-β1,PDGF-BB and bFGF in study group were significantly higher than that in control group (P ＜0.05).Conclusions Concentration of TGF-β1,PDGF-BB and bFGF was positively associated with area of transcervical resection of intrauterine adhesion,those cytokines might be involved in reformatin of intrauterine-adhesion.

Objective To observe the hemodynamic change of small artery in patients with type 2 diabetes. Methods We measured peak systolic velocity (PSV), end-diastolic velocity (EDV), pulse index (PI), resistance index (RI) of ocular artery (OA), central retinal artery (CRA), finger artery, dorsal pedis artery and interlobar artery of patients with early-stage type 2 diabetes. Results The decrease in PSV and EDV values occurred early in CRA of type 2 diabetics while RI and PI values increased. Conclusion The hemodynamic change of CRA occurs earliest among general small arteries of type 2 diabetic patients.

Aim To study the effects and mechanism of Guanxinkang capsules on myocardial injury induced by isoproterenol in rats.Methods 30 male Wistar rats were randomly divided into 3 groups:control group,acute myocardial ischemia group and Guanxinkang capsules group.Rats were given injections of ISO subcutaneously.Electrocardiography were performed,serum and myocardium creatine kinase(CK),creatine kinase MBmass(CK-MB),lactate dehydrogenase(LDH) and nitric oxide(NO) were detected,and the levels of NO and endothelins(ET) in myocardium were measured as well.Results The rising degree of ST-T obviously decreased in Guanxinkang capsules group,and the levels of serum CK,CK-MB and LDH decreased in Guanxinkang capsules group,compared with those of acute myocardial ischemia group(P

Cerebral Infarction ; drug therapy ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Humans ; Phytotherapy ; methods

Objective To evaluate total laparoscopic splenectomy combined with pericardial devascularization. Methods In this study,40 patients with portal hypertension and hypersplenism underwent total laparoscopic splenectomy combined with pericardial devascularization,in which 26 cases had a history of up-GI bleeding. Results Procedures were successful in 36 patients.There was four conversions to open surgery.The operations were completed in a mean time of (224 ± 54) min,with a mean estimated blood loss of (296 ± 209) ml and with no serious complication. Conclusion Total laparoscopic splenectomy combined with pericardial devascularization is a safe and effective available minimally invasive procedure in patients with portal hypertension and hypersplenism.

Objective To analyze the diagnostic value of serum procalcitonin ( PCT ) for early postoperative bacterial infection after pediatric living donor liver transplantation.Methods A retrospective study was conducted in pediatric patients after living donor liver transplantation recipients admitted to department of critical care medicine of Beijing Friendship Hospital affiliated to Capital Medical University during June 2013 to October 2015.According to the clinical data , all pediatric patients were divided into infection group(n=60) and non-infection group (n=100).Primary disease, PCT post operation day 1 to day 5 for non-infection group and day 1 to day 9 for infection group , temperature , white blood cell , cold ischemia time, warm ischemia time, operation time, volume of blood loss during operation were recorded.All parameters above were compared between groups.Receiver operating characteristic ( ROC) curve was plotted, and the diagnostic value of PCT was evaluated.Results PCT of both groups were elevated after liver transplantation , there was a markedly resolution in non-infection group within 48 to 72 hours.PCT of pediatric patients with bacterial infection was significantly higher than that of non-infected patients , and the difference was of greatly significant (4.62 ±1.39) ng/ml vs (0.85 ±0.19) ng/ml,t=26.56,P=0.00.ROC curve showed that the peak level of PCT might be valuable in the diagnosis of bacterial infection ( AUC=0.985).There was no significant difference of cold ischemia time [(109.92 ±19.22) min vs (108.04 ± 13.20) min, t=1.05, P=0.29], warm ischemia time[(1.49 ±0.17) min vs (1.52 ±0.12) min, t=1.08, P=0.28], operation time[(8.01 ±0.77)vs (8.00 ±1.05) h, t=0.06, P=0.94], WBC[(8.95 ±1.69) ×109/L vs (8.98 ±2.00) ×109/L,t=-0.08, P=0.93]and body temperature[(37.5 ±0.7) vs (37.5 ±0.8) ℃,t=-0.05, P=0.96] on the first day after surgery between infection and non-infection groups.Amount of bleeding in infection group was higher than that of non infection group [ ( 650.87 ± 90.36) ml vs (240.29 ±67.67) ml, t=32.33, P=0.00], there was longer length of ICU stay in the infection group[(11.01 ±1.81)d vs (6.03 ±1.65)d, t=17.78, P=0.00].Conclusion Peak PCT level was a valuable indicator for early postoperative bacterial infection after pediatric living donor liver transplantation.

Objective To investigate the methylation status of the promoter of RASSF1A gene in the tissues of bladder cancer and renal carcinoma and its role in tumorigenesis. Methods Methylation specific PCR (MSP) was used to examine the methylation status of RASSF1A in bladder cancer and renal carcinoma.The specimen included 45 cases of bladder cancer tissues and paired adjacent normal tissues from cystectomy,9 cases of bladder cancer tissues from microresection,3 cases of normal bladder tissues from people without tumor,12 cases of renal carcinoma tissues and paired adjacent normal tissues. Results 30 of 54(55.6%)bladder cancer tissues showed RASSF1A gene aberrant methylation.Of which,26 of 45 ( 57.8 %) bladder cancer tissues from cystectomy showed aberrant methylation,while one of the paired normal tissues showed gene methylation,4 of 9 (44.4%) bladder cancer tissues from microresection showed gene methylation.Eight of 12(66.7%)renal carcinoma tissues showed aberrant methylation,while none of the paired adjacent normal tissues showed gene methylation.None of the three normal bladder tissues showed aberrant methylation.There was no significant correlation between the methylation status and the clinical pathological parameters in bladder cancer tissues from cystectomy,though there was a trend that more frequent methylation was detected in higher stage bladder cancer. Conclusions Hypermethylation of RASSF1A gene in bladder cancer and renal carcinoma suggeste its possible role in the tumorigenesis of both tumors.

Objective To investigate the promoter methylation status and the transcription level of BLU in bladder carcinoma and their significance.Methods The methylation status of promoter and the transcription level of BLU were examined by methylation specific PCR(MSP) and RT-PCR in bladder cancer tissues from 54 patients,adjacent normal tissues from 45 cases and normal bladder tissues from 3 noncancerous patients.Results ① 31.5%(17/54) bladder cancer tissues showed BLU hypermethylation;neither the paired adjacent normal tissues nor the three normal bladder tissues showed BLU hypermethylation.② There was no significant correlation between the methylation status and the clinical pathological parameters in bladder cancer.③ The expression of BLU was absent in 42.2%(19/45) of bladder carcinoma from cystectomy.④ Loss of BLU expression was associated with the stage and grade of the tumor(P

Child, Preschool ; Female ; Humans ; Infant ; Male ; Mycophenolic Acid ; adverse effects ; analogs & derivatives ; therapeutic use ; Nephrotic Syndrome ; drug therapy ; Treatment Outcome

Objective To study the anti-cancer constituents from Tripterygium wilfordii. Methods Chemical constituents were isolated by repeated column chromatography (silica gel, Toyopearl HW-40 C and preparative HPLC), their structures were elucidated on the basis of spectroscopic methods, and the anti-cancer activity was screened by MTT method. Results Five diterpenes, 3-epi-triptobenzene B (Ⅰ), 3?, 14-dihydroxy-abieta-8, 11, 13-triene (triptobenzene B,Ⅱ), wilforol E (Ⅲ), triptohairic acid (Ⅳ), and 11-hydroxy-14-methoxy-18(4→3)-abeo-abietan-3, 8, 11, 13-tetraen-18-oic-acid (hypoglic acid, Ⅴ) were isolated from T. wilfordii. Conclusion Compound Ⅰ is a new compound named as triptobenzene L, compound Ⅳ is isolated for the first time. The compounds Ⅰ-Ⅴ show the positive anti-cancer effects on HeLa and L929 cell lines.