OBJECTIVE: To evaluate the in vitro and in vivo antibacterial activities of cefodizime from both home and abroad. METHODS: The minimal inhibitory concentrations (MICs) of domestic and imported cefodizime, cefotaxime sodium against 300 clinical isolates of bacteria were tested by standard agar dilution method and the test tube dilution method. The in vivo antibacterial activities of cefodizime were determined in mice with experimental systemic infection caused by E.coli, K. pneumoniae and S.aureus. The values of ED50 were calculated by Bliss method. RESULTS: Both domestic and imported cefodizime had remarkable potency against majority gram- negative bacteria and gram- positive bacteria, including pneumococcus and beta streptococcus, and the MIC90 of cefodizime stood at 0.012～1.0?g?mL-1; and they showed medium grade antibacterial activity against bacillus cloacae, bacillus aerogenes and serratia but mild effects on MSSA and S.epidermidis, and the MIC50 stood at 4?g?mL-1. In this trial, bacillus aeruginosus, acinetobacter, enterococcus and MRSA showed resistance against the domestic and imported cefodizime. The in vivo antibacterial activities of domestic and imported cefodizime were similar and remarkable in mice infected with E.coli, K.pneumoniae and S.aureus. CONCLUSION: Domestic and imported cefodizime had excellent and similar antibacterial activity.

Burns ; complications ; metabolism ; Humans ; Inflammation ; etiology ; metabolism ; prevention & control

Burns ; complications ; immunology ; therapy ; Humans ; Immune System Diseases ; etiology ; therapy ; Nutritional Support

inflammatory pseudotumor. Conclusions The number of pleural is closely relative to the shape size and position. The occurrence rate of pleural indentation is closely relative to the distance between SPN and pleural,and the nature of SPN has correlation with pulling pleural.

Objective To investigate somatostatin receptor subtype 2 (SSTR2) mRNA expression and its correlation with proliferating cell nuclear antigen (PCNA) in human colorectal cancer. Methods A total of 60 colorectal cancer samples were analyzed. The SSTR2 mRNA expression was examined by in situ hybridization (ISH) using multiphase oligoprobes. The PCNA were detected by immunohistochemical staining. A computerized image analysis system was utilized to estimate the relative contents of SSTR2 mRNA. Results The positive rate of expression of SSTR2 mRNA and relative contents in early and well-differentiated colorectal cancer were higher than those in advanced and poorly differentiated lesion. A negative correlation between SSTR2 mRNA expression and PCNA was found in colorectal cancer. Conclusion In the samples of colorectal cancer, the expression of SSTR2 mRNA is correlated with the degree of differentiation and clinical stage, and it plays an important role in reflecting the degree of malignancy and prognosis.

OBJECTIVE: To determine Plumbago content in Herba Plumbaginis Zeylanicae under different growing conditions and to provide experimental data for its domestication. METHODS: High performance liquid chromatography was used to determine Plumbago contents in the roots, stems and leaves of home-grown Herba Plumbaginis Zeylanicae. RESULTS: Plumbago content in Herba Plumbaginis Zeylanicae grown in mellow and clay soil was higher than in those grown in sand and crude soil. Potasium-fertilized and nitrogen-fertilized Herba Plumbaginis Zeylanicae contained higher Plumbago than those fertilized by phosphorus. Plumbago content was the highest in Herba Plumbaginis Zeylanicae grown in full light. Plumbago contents in roots, stems, and leaves of Herba Plumbaginis Zeylanicae grown under different bred conditions did not vary much. CONCLUSION: The research group's preliminary studies indicated that Plumbago was the main effective ingredient having anti-cancer and anti-inflammation bioactivity. Clay soil, cuttings, potassium fertilizer and nitrogen fertilizer, and full light were thought as ideal conditions for increasing Plumbago content. High quality Herba Plumbaginis Zeylanicae can be grown under these conditions. Copyright 2012 by the Chinese Pharmaceutical Association.

Adult ; Aged ; Cervical Intraepithelial Neoplasia ; pathology ; surgery ; Cervix Uteri ; pathology ; surgery ; Conization ; methods ; Female ; Follow-Up Studies ; Humans ; Hysterectomy ; methods ; Middle Aged ; Neoplasm Recurrence, Local ; Neoplasm, Residual ; pathology ; surgery ; Uterine Cervical Neoplasms ; pathology ; surgery

96% and with the same bio-activity as unlabeled Huwen toxin-1; Radioactivity detected in epidural space was 38% of injected radioacti vity at 10 min after epidural injection, which demonstrated successful administr ation into epidural space; The maximum serum concentration after epidural or iv administration of [ 125 I]labeled Huwentoxin-1 were determined to be (0 70?0 04) MBq?L -1 and (4 98?0 58) MBq?L -1 , respectively, a t the maximum serum concentration times of 30 min and 2 min. Terminal T 1/2 after epidural or iv administration were (10 36?0 27) h or (11 03?1 16) h, respectively. Cls was (1 29?0 07) L?h -1 ?kg -1 or (1 25? 0 23) L?h -1 ?kg -1 , respectively. Bioavailability after epidural a dministration was(95?5)%. CONCLUSION Concentration-time cur ves of [ 125 I] labeled Huwentoxin-1 after two routes were different. The degradation profiles after epidural and iv injection supported the using of HWTX-1 as analgesic by epidural administration.

AIM:To investigate the role of danshensu on Smad signal transduction in rat hepatic stellate cells(HSCs)stimulated with transforming growth factor(TGF-?1).METHODS:The rat HSCs was isolated with collagenase by in situ-liver recirculation perfusion and cultured in vitro.MTT colorimetric assay was used to detect proliferation of HSCs treated with different concentration of danshensu.The expressions of ?-SMA and T?R I and II were observed by immunocytochemitry,indirect immunofluorescent staining and Western blotting when HSCs stimulated with TGF-?1 and with different concentrations of danshensu for 24 h.RESULTS:(1)Danshensu at the concentration from 0.0625 mmol/L to 1 mmol/L prevented the proliferation of HSCs in a dose-dependent manner(P

Chronic Disease ; Diagnosis, Differential ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Gastritis ; diagnosis ; drug therapy ; Humans ; Medicine, Chinese Traditional ; Phytotherapy ; Reference Standards