The zymograms of lactate dehydrogenase (LDH) and esterase (EST) isoenzymes in different physiologic state from two species of Anopheles mosquitoes were studied with polyacrylamide gel electro -phoresis. Some zymogram variation of LDH from single female mosquito was found after engorged blood meals for 48 h, that consisted of two main bands whose relative mobility (Rm) was different. The isozymograms of EST showed some similar characteristics of sibling which divided into three groups ESTⅠ, ESTⅡand ESTⅢ, respectively. The two species of mosquitoes could be differentiated from each other by the particular band in EST Ⅱ group of An. anthropophagus.

A simple and reliable procedure for the isolation of pure DNA from human fetal liver is described.A high pure and natural DNA with an average molecular weight of 4 ?106 can be easily obtained after the sample is treated with SDS and trypsin and fractionated by a sepharose 4B column.The product meets the requirement for general research work of molecular biology.

This paper is to report the results of the clinical application of the Shexiang hemostasis-removing plaster. 265 cases of dermatohemostasis including chilblain, erythema multiforme, erythema nodosum, and so on were treated locally with Shexiang hemostasis-removing plaster. 20 cases of dermatohemostasis were treated with Shexiang analgesic plaster and served as control.It was found the rates of marked improvement and improvement after the application of Shexiang hemostasis-removing plaster were 85.61% and 96.60%, while those after Shxiang analgesic plaster treatment were 65% and 90% respectively. It is bovious that Shexiang hemostasis-removing plaster is more effective in treating dermatohemostasis than Shexiang analgesic plaster. The difference between the 2 is statistically significant(P

Acute kidney injury(AKI) can lead to remote organ damage.The specific mechanism by which the AKI results in distant organ damage is yet to be determined.In animal models,it has become increasingly clear that AKI is not an isolated event but results in remote organ dysfunction involving the heart,lungs,liver,and brain.This review summarizes the effects of AKI on remote organs.

Coronal restoration of endodontically treated teeth may be viewed as one of the main parameters that influence the outcome of endodontic treatment. The purposes of restoring endodontically-treated teeth include preventing recontamination of the root canal system and periapical area, replacing the compromised dental hard tissue, restoring the coronal morphology and function, providing necessary strength for the restoration/tooth complex for functional stress, and avoiding crown and/or root fracture. This article reviewed recent researches on the restoration of endodontically treated teeth, provided evidence for clinical practice on topics as when to restore them, basic principles to be considered during treatment planning, and specific restoration options for both anterior and posterior teeth under different functional occulsal load conditions. Several issues should be taken into account during the decision making process, such as remaining tooth tissue, functional masticatory forces, comprehensive oral rehabilitation, and esthetic requirements.
Decision Making ; Dental Restoration, Permanent ; Humans ; Root Canal Therapy ; Tooth Crown ; Tooth, Nonvital

Bronchoalveolar Lavage ; methods ; Humans

Asthma ; epidemiology ; prevention & control ; therapy ; Child ; China ; epidemiology ; Drug Therapy, Combination ; Humans ; Immunotherapy

Bacterial Typing Techniques ; methods ; Communicable Diseases ; diagnosis ; microbiology ; virology ; Epidemiologic Methods ; Humans ; Molecular Diagnostic Techniques ; methods ; Oligonucleotide Array Sequence Analysis ; methods

Adolescent ; Age Factors ; Biopsy ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Kidney ; diagnostic imaging ; pathology ; physiopathology ; Kidney Failure, Chronic ; etiology ; pathology ; Male ; Multicystic Dysplastic Kidney ; complications ; Nephritis ; complications ; Nephrotic Syndrome ; complications ; Radiography

Child ; Chronic Disease ; Cough ; diagnosis ; therapy ; Guidelines as Topic ; Humans ; Pediatrics