Rho/Rho-kinase signaling pathway is a ubiquitous one in vivo.It plays a role of "molecular switch"by regulating the polymeric state of intracellular actin cytoskeleton,and participates in the regulation of various cellular functions.Rho/Rho-kinase signaling pathway plays an important role in the risk factors.pathogenesis and pathophysiological processes of ischemic cerebrovascular disease.The inhibition of Rho/Rho-kinase signaling pathway may achieve a significant neuroprotective effect.

Dentists

Alprostadil ; administration & dosage ; adverse effects ; therapeutic use ; Hearing Loss, Sudden ; drug therapy ; Humans ; Injections, Intravenous ; Lower Extremity ; Male ; Middle Aged ; Pain ; etiology

Humans ; Positive-Pressure Respiration ; Respiration, Artificial ; Severe Acute Respiratory Syndrome ; therapy

Aged ; Cardiovascular Diseases ; diagnosis ; etiology ; Cerebrovascular Disorders ; diagnosis ; etiology ; Diagnostic Errors ; Female ; Humans ; Laryngitis ; complications ; diagnosis ; Middle Aged

Objective: To investigate the expression of hypoxia inducible factor-1α(HIF-1α) in HepG2 cells cultured under hypoxia for different time periods, and to assess its relationship with vascularization and cell apoptosis. Methods: HepG2 cells were cultured in a medium containing cobalt chloride (125 μmol/L) for different time periods (0, 1, 2, 4, 6, and 8 hours). RT-PCR was used to measure the expression of HIF-1α mRNA, VEGF mRNA, and bcl-2 mRNA, and bax mRNA at the above time points; Western blot was used to determine the expression of Caspase-3 protein and the activity of Caspase 3 enzyme. Results: After 1-2 hour culture under hypoxia, the expression of HIF-1α mRNA, VEGF mRNA, and bcl-2 mRNA began to increase gradually, peaked at 4 h, and then decreased, but were still higher than that before culture. There was no obvious change in the expression of bax mRNA and the ratio of bcl-2 to bax mRNA expression had a similar change with that of bcl-2 mRNA expression. The changes of Caspase-3 protein/mRNA and Caspase-3 enzyme activity were contrary to that of HIF-1α. Conclusion: HIF-1α may inhibit the apoptosis of hepatic cancer cells through regulating the expression of VEGF, bcl-2, bax, and Caspase-3, and the inhibition is related to the severity of hypoxia.

Adenoma ; Diagnosis, Differential ; Hamartoma ; Humans ; Nasal Cavity ; surgery ; Paranasal Sinuses ; Respiratory Mucosa

Aged ; Aged, 80 and over ; Alzheimer Disease ; metabolism ; pathology ; Brain ; metabolism ; Dentate Gyrus ; metabolism ; Dynamin I ; metabolism ; Hippocampus ; metabolism ; Humans ; Monomeric Clathrin Assembly Proteins ; metabolism ; Neuropil ; metabolism ; Synaptophysin ; metabolism

Objective The present study was designed to compare the effects of different intra-abdominal pressure and the duration of pneumoperitoneum on splanchnic perfusion during laparoscopic cholecystectomy. Methods Fifty ASA Ⅰ or Ⅱ patients aged