Dentists

Rho/Rho-kinase signaling pathway is a ubiquitous one in vivo.It plays a role of "molecular switch"by regulating the polymeric state of intracellular actin cytoskeleton,and participates in the regulation of various cellular functions.Rho/Rho-kinase signaling pathway plays an important role in the risk factors.pathogenesis and pathophysiological processes of ischemic cerebrovascular disease.The inhibition of Rho/Rho-kinase signaling pathway may achieve a significant neuroprotective effect.

Aged ; Cardiovascular Diseases ; diagnosis ; etiology ; Cerebrovascular Disorders ; diagnosis ; etiology ; Diagnostic Errors ; Female ; Humans ; Laryngitis ; complications ; diagnosis ; Middle Aged

Alprostadil ; administration & dosage ; adverse effects ; therapeutic use ; Hearing Loss, Sudden ; drug therapy ; Humans ; Injections, Intravenous ; Lower Extremity ; Male ; Middle Aged ; Pain ; etiology

Humans ; Positive-Pressure Respiration ; Respiration, Artificial ; Severe Acute Respiratory Syndrome ; therapy

Objective: To investigate the expression of hypoxia inducible factor-1α(HIF-1α) in HepG2 cells cultured under hypoxia for different time periods, and to assess its relationship with vascularization and cell apoptosis. Methods: HepG2 cells were cultured in a medium containing cobalt chloride (125 μmol/L) for different time periods (0, 1, 2, 4, 6, and 8 hours). RT-PCR was used to measure the expression of HIF-1α mRNA, VEGF mRNA, and bcl-2 mRNA, and bax mRNA at the above time points; Western blot was used to determine the expression of Caspase-3 protein and the activity of Caspase 3 enzyme. Results: After 1-2 hour culture under hypoxia, the expression of HIF-1α mRNA, VEGF mRNA, and bcl-2 mRNA began to increase gradually, peaked at 4 h, and then decreased, but were still higher than that before culture. There was no obvious change in the expression of bax mRNA and the ratio of bcl-2 to bax mRNA expression had a similar change with that of bcl-2 mRNA expression. The changes of Caspase-3 protein/mRNA and Caspase-3 enzyme activity were contrary to that of HIF-1α. Conclusion: HIF-1α may inhibit the apoptosis of hepatic cancer cells through regulating the expression of VEGF, bcl-2, bax, and Caspase-3, and the inhibition is related to the severity of hypoxia.

Aged ; Aged, 80 and over ; Alzheimer Disease ; metabolism ; pathology ; Brain ; metabolism ; Dentate Gyrus ; metabolism ; Dynamin I ; metabolism ; Hippocampus ; metabolism ; Humans ; Monomeric Clathrin Assembly Proteins ; metabolism ; Neuropil ; metabolism ; Synaptophysin ; metabolism

Objective To assess the influence of changes in alveolar ventilation on gastro-intestinal perfusion measured via a nasogastric tonometer.Methods Sixteen ASA Ⅰ - Ⅱ patients ( 7 male, 9 female) aged 15-67 yr, undergoing elective intracranial operation were included in this study. The patients were premedicated with oral ranitidine 150 mg and intramuscular phenobarbital 0.1 g and atropine 0.5 mg. Anesthesia was induced with midazolam 1-2 mg, fentanyl 2 ?g? kg-1 , propofol 1.5-2.0 mg ? kg-1 and vecuronium 0.1 mg ? kg-1 , and maintained with 0.5%-2.0% isoflurane inhalation and intermittent iv boluses of fentanyl and vecuronium. The patients were intubated and mechanically ventilated after induction of anesthesia. The ventilatory settings were : RR 10 bpm; FGF 1 L? min-1; I: E = 1:2; the initial VT was 7 ml? kg-1 (T1 ) which was gradually increased to 8 ml ?kg-1(T2), 9ml?kg-1(T3) and 10 ml?kg-1 (T4) and then returned to 7 ml ? kg-1 again. Each VT was maintained for 1 h. A nasogastric tonometer (Tonocap, Datex-Ohmeda, Finland) was inserted into stomach and automatically measured gastric intramucosal CO2 tension (PgCO2) every 10 min. Radial artery was cannulated for direct MAP monitoring and blood sampling. Blood gas analysis was performed every hour at various VT levels. Gastric-to-arterial pH gap and gastric-to-arterial PCO2 gap [P(g-a)CO2] were calculated.Results PgCO2 decreased during T2_4 (hyperventilation phase) (P

Objective The present study was designed to compare the effects of different intra-abdominal pressure and the duration of pneumoperitoneum on splanchnic perfusion during laparoscopic cholecystectomy. Methods Fifty ASA Ⅰ or Ⅱ patients aged