Adolescent ; Adult ; Amnion ; transplantation ; Blood Transfusion, Autologous ; Eye Burns ; therapy ; Humans ; Male ; Middle Aged ; Occupational Injuries ; therapy ; Young Adult

Objective: To study the protective effect of the water extract from Paeoniae Radix Rubra (WEPRR) against myocardial ischemia in mice induced by isoproterenol (ISO). Methods: The mice were randomly divided into six groups: positive control, normal control, model, low-, mid-, and high-dose [0.3, 0.6, and 1.2 g/(kg·d)] WEPRR groups. The mice in WEPRR groups were ig administered with WEPRR, the mice in the positive control group were ig administered with 0.98% Di'ao Xinxue Kang [(0.195 g/(kg·d)], and the mice in normal and model groups were ig administered with the same volume of physiological saline once daily for consecutive 11 d. On the day 7 from the beginning of the ig administration, the mice in the model and WEPRR groups were ip perfused with 0.02 g/(kg·d) ISO. After 15 min of the last medication, the mice were anesthetized with isoflurane gas, the blood was collected through venous sinus of eye orbit, then the mice were killed. The heart tissues were rapidly removed from the mice, washed in physiological saline, soaked in filter paper, and stored in -80 C until use. The activities of lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), creatine kinase (CK) in serum, superoxide dismutase (SOD), and the contents of malondialdehyde (MDA) in the heart of mice were determined, respectively. Results: Compared with the model group, the activities of LDH, CK-MB, and CK in serum, and the SOD of mice in the positive control and WEPRR groups were increased and the content of MDA in heart was decreased. Conclusion: The WEPRR has the cardioprotective activities on ISO-induced myocardial ischemia. © 2013 Tianjin Press of Chinese Herbal Medicines.

Grasses infected by endophytes have an extraordinary impact on the ecology and economy of pasture and turf. In this review, we presented recent research progress on origin and evolution, biological and ecological impact, and perspective of the association in order to make full use of the widely distributed endophyte resources.

Objective To explore the echocardiography characteristics of non-compaction of ventricular myocardium(NVM),and assess its value in the diagnosis of NVM.Methods Philips SONOS 5500 color Doppler ultrasound system with 2-4 MHz transducers was used in 9 patients with NVM.Results In all 9 patients,the presence of multiple trabeculations and multiple deep intertrabecular Space communicating with the ventricular cavity was demonstrated by color Doppler imaging.Both ventricles were involved in 1 patient,only the left ventricle in 7 patients,and right ventricle in 1 patient.Conclusions NVM has typical ultrasonic appearance on echocardiography.It Can find out the NVM manifestations even if patients do not have any symptoms.It is a reliable and convenient way for diagnosing NVM.

Adult ; Antibiotics, Antineoplastic ; therapeutic use ; Benzamides ; Blast Crisis ; drug therapy ; Drug Resistance, Neoplasm ; Humans ; Imatinib Mesylate ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; drug therapy ; Male ; Piperazines ; pharmacology ; Pyrimidines ; pharmacology ; Sirolimus ; therapeutic use

Acupuncture Therapy ; Adult ; Female ; Humans ; Thyroid Neoplasms ; surgery ; therapy

Objective To evaluate the performance of microRNA‐21(miRNA‐21) for the diagnosis of esophageal cancer .Meth‐ods Published literatures ,investigating the diagnostic performance of miRNA‐21 in esophageal cancer ,were screened .QUADAS‐2 software was used to evaluate the quality of related literatures .All analysis about relevant data was accomplished by using Meta‐DiSc statistical software .The sensitivity ,specificity ,positive likelihood rate ,negative likelihood rate and diagnostic odds ratio were pooled ,using corresponding effects models .Results Four studies met the inclusion criteria ,accumulating 202 esophageal cancer pa‐tients and 124 healthy controls .The summary estimates for miRNA‐21 in the diagnosis of esophageal cancer were with diagnostic sensitivity of 0 .85 and specificity of 0 .65 .The area under summary receiver operating characteristic curve was 0 .817 4 .Conclusion miRNA‐21 might be a novel biomarker for the diagnosis of esophageal cancer ,and could be used for screening and auxiliary diag‐nosis of esophageal cancer .

A PCR assay used for detection and quantification of Neotyphodium lolii in tissues of the grass Lolium perenne L. was developed. Compared with the Microscopic analysis of stained tissues, this method was more accurate. For quantitative applications, real time PCR was used to quantify Neotyphodium lolii in Lolium perenne L.. The results showed that there were some variations between different individuals, but no variance existed between different tillers from the same plant. Thus it can be concluded that fungal content in the plant is not only associated with species/variety specific, but also with the genotype of the host plant as well.

To observe the morphology of corneal endothelial cells after femtosecond laser in situ keratomileusis ( Femto-LASlK) .METHODS:From May to September in 2013, 88 eyes of 45 patients with myopia who accepted Femto-LASlK were enrolled in this study. The morphology of central corneal endothelial cells was measured by a non-contact corneal endothelial cell analyzer before, 1mo and 1a after surgery. The number, density, average size, size standard deviation, size coefficient of variation and hexagonality of the corneal endothelial cells were observed. All the measurements were analyzed by statistical analysis.RESULTS: All patients underwent operation smoothly, and no complication was observed during and after surgery;One month after surgery, the endothelial cell density (ECD) was 2815. 34±297. 07/mm2, which had a 2. 64% decrease compared with preparative ECD (t=4. 60, P= 0. 00), there was no statistical significance between ECD measurements of 1mo and 1a after surgery ( P>0. 05 ); One month after surgery, the size standard deviation ( SSD ) was 118. 47 ± 31. 58μm2 , which increased significantly compared with the preoperative SSD(t=-3. 87, P=0. 03), there was no statistical significance between SSD measurements of 1mo and 1a after surgery ( P>0. 05 );After surgery, the number, hexagonality, average size and size coefficient of variation of the corneal endothelial cells didn't change statistically(P>0. 05).CONCLUSlON: ln the early period after Femto-LASlK, the ECD decreased slightly, however this kind of surgery did not have significant harm to the function of corneal endothelial cells, and the surgery didn't cause the progressive loss of corneal endothelial cells.