1.Serum progesterone levels in early pregnant women and its relation to luteal maintenance therapy for pregnancy protection
Chinese Journal of General Practitioners 2015;14(4):269-273
Objective To investigate serum levels of β-human chorionic gonadotrophin (β-HCG) and progesterone (P) in early pregnant women,and their relation to luteal maintenance therapy for early pregnancy protection.Methods One hundred and thirty five infertility women treated in Department of Gynecology and Obstetrics of Beijing Chaoyang Hospital from January 2007 to December 2011.Total 150pregnancy cycles,including 84 with intrauterine insemination (IUI) and 66 with natural conception,were divided into two groups:normal intrauterine pregnancy group (group A,n =80) and early pregnancy loss group (group B,n =70).Medical history,ultrasonic findings,serum female hormone,P and β-HCG levels at early pregnancy stage were analyzed.Results The age of group A and group B was (30.0 ± 3.9) years and(30.7 ± 4.9) years,respectively (P > 0.05).The follicle-stimulating hormone/luteinizing hormone in group A and group B was 1.57 ±0.96 and 1.56 ± 1.08 ; the estradiol levels in two groups were (152 ±66) pmol/L and (147 ± 69) pmol/L,respectively (both P > 0.05).There were no differences in dominant follicles and endometrial thickness between groups A and B (P > 0.05).Ovulation promotion and luteal support treatments were adopted in both groups:50% (40/80) of cycles in group A received ovulation promotion,73% (58/80) of cycles received luteal support,while 44% (31/70) and 76% (53/70) received in groups B,respectively.The levels of serum progesterone in group A during 14-21 d,22-27 d and ≥ 28 d after ovulation were higher than those in group B at each time points (P < 0.0l).There were no significant differences in serum progesterone levels between women with luteal support treatment and those without luteal support treatment in both groups (P > 0.05).Conclusion Dynamic monitoring of serum progesterone level in early pregnancy may be used as an auxiliary index for prediction of pregnancy outcome,but it may not be an indicator for luteal maintenance therapy.
2.The value of early assessment in trauma patients by ultrasound
Chinese Pediatric Emergency Medicine 2015;22(6):370-374
Trauma is one of the main reasons for the death and disability in children.Ultrasonic tech-nology has been applied in the early assessment and diagnosis of trauma.it is quick,effective,non-invasive, fast imaging,mobile and suitable for different patients and different parts of the body.It has been used to as-sess the organs in patients with severe trauma widely.
3.Relevance between Gastrointestinal Microbiota and Helicobacter pylori Eradication Therapy
Chinese Journal of Gastroenterology 2015;(10):632-634
Gastrointestinal microbiota is a huge and complex system that has multiple influential factors. As an exogenous pathogen,Helicobacter pylori(Hp)may interact with gastrointestinal microbiota. It has been revealed that Hp infection led to an increase in Lactobacillus acidophilus in intestinal tract and a decrease in Actinobacteria,Bacteroidetes and Firmacutes in stomach. Proton pump inhibitors and antibiotics,the major components of eradication regimens for Hp infection,may also have an impact on gastrointestinal microbiota. On the other hand,gastrointestinal microbiota interferes with Hp’s attachment into gastric mucosa. Probiotics combined with eradication therapy could benefit the eradication rate of Hp infection and reduce adverse events.
4.Clinical Research on Three Ingredient Preparations of Calcium Hydroxide for Root Canal Disinfection
China Pharmacy 2007;0(35):-
0.05),but the above 3 groups were superior to FC group in the EIP incidence,pain degree and curative effect (P
5.Expression and clinical significance of transcription factor Sox2 in human prostate cancer
Zhenyu ZHANG ; Yufeng ZHANG ; Hongwei ZHANG
Journal of International Oncology 2014;41(11):875-878
Objective To study the expression of Sox2 in human prostate cancer and pericarcinomatous tissue,and the relation between its expression and the pathological type and clinical stage of prostate cancer.Methods Immunohistochemisty,Western blot and reverse transcription-polymerase chain reaction (RT-PCR)were used to detect the expression of Sox2 in 86 cases of prostate cancer tissues and 40 cases of pericarcinomatous tissues.The relation between Sox2 expression and the pathological type and clinical stage of prostate cancer was analyzed.Results The expression of Sox2 in prostate cancer tissue (71.9%) was significantly higher than that in pericarcinomatous tissue (7.5%),and the difference was statistically significant (x2 =53.98,P < 0.05).Sox2 expression in prostate cancer tissues was significantly related to Gleason score (x2 =19.49,P < 0.05).The positive rate of Sox2 in lymphatic metastasis group and negative lymphatic metastasis group was 92.3% and 73.3% respectively,and the difference was statistically significant (x2 =3.95,P < 0.05).While there was no significant difference among clinical stages (x2 =0.16,P > 0.05).Moreover,Western blot and RT-PCR revealed that the expression of Sox2 in prostate cancer tissue was significantly higher than that in pericarcinomatous tissue (t =-2.93,P < 0.05 ; t =3.29,P < 0.05).Conclusion The high expression of Sox2 is closely related to the Gleason score and lymphatic metastasis of prostate cancer,but not to the clinical stage.The overexpression of Sox2 may play an important role in formation,differentiation and metastasis of prostate cancer.
6.Reflection on the new training model of the research-oriented graduates in affiliated hospitals of medical universities
Long BI ; Shu HE ; Zhenyu ZHANG
Chinese Journal of Medical Education Research 2012;11(2):134-136
The amount of the research-oriented graduates is increasing in affiliated hospitals of medical universities.To create an effective training model can not only help students to succeed but also contribute great to the research level of hospitals.In this study,we summarized our experience,such as individualized teaching,the unity of thinking and performing,words and deeds as well as unity of clinic and research on the formation of research ideas,attitudes and styles.Based on our experiences we hope to provide meaningful methods for the training of medical graduates.
7.Enzymatic production of α-ketoglutaric acid by L-glutamate oxidase from L-glutamic acid.
Panqing NIU ; Zhenyu ZHANG ; Liming LIU
Chinese Journal of Biotechnology 2014;30(8):1318-1322
We produced α-ketoglutaric acid (α-KG) from L-glutamic acid, using enzymatic transformation approach with L-glutamate oxidase (LGOX). First, wild strain Streptomyces sp. FMME066 was mutated with NTG, a genetically stable mutant Streptomyces sp. FMME067 was obtained. Under the optimal nutrition conditions with fructose 10 g/L, peptone 7.5 g/L, KH2PO4 1 g/L and CaCl2 0.05 g/L, the maximum LGOX activity reached 0.14 U/mL. The LGOX was stable to pH and temperature, and Mn2+ had a stimulating effect. Finally, after 24 h enzymatic conversion under the optimal conditions, the maximum titer of α-KG reached 38.1 g/L from 47 g/L L-glutamic acid. Enzymatic transformation by LGOX is a potential approach for α-KG production.
Amino Acid Oxidoreductases
;
metabolism
;
Fermentation
;
Glutamic Acid
;
metabolism
;
Ketoglutaric Acids
;
metabolism
;
Streptomyces
;
genetics
;
metabolism
8.Clinical significance of preventive treatment of thrombosis for patients undergoing gynecological surgery with high risk factors
Bailiu YANG ; Zhenyu ZHANG ; Shuli GUO
Chinese Journal of Obstetrics and Gynecology 2009;44(8):570-573
e factors of elder age, abdominal surgeries and malignant tumor.
9.Development and validation of a GC-FID method for quantitative analysis of oleic acid and related fatty acids☆
Honggen ZHANG ; Zhenyu WANG ; Oscar LIU
Journal of Pharmaceutical Analysis 2015;5(4):223-230
Oleic acid is a common pharmaceutical excipient that has been widely used in various dosage forms. Gas chromatography (GC) has often been used as the quantitation method for fatty acids normally requiring a derivatization step. The aim of this study was to develop a simple, robust, and derivatization-free GC method that is suitable for routine analysis of all the major components in oleic acid USP-NF (United States Pharmacopeia-National Formulary) material. A gas chromatography-flame ionization detection (GC-FID) method was developed for direct quantitative analysis of oleic acid and related fatty acids in oleic acid USP-NF material. Fifteen fatty acids were separated using a DB-FFAP (nitroterephthalic acid modified polyethylene glycol) capillary GC column (30 m × 0.32 mm i.d.) with a total run time of 20 min. The method was validated in terms of specificity, linearity, precision, accuracy, sensitivity, and robustness. The method can be routinely used for the purpose of oleic acid USP-NF material analysis.
10.Lung adenocarcinoma progression schema
Zhenyu YANG ; Zhiwen FAN ; Lei ZHANG
Journal of International Oncology 2013;(2):120-122
Lung adenocarcinoma is usually thought to follow a linear multistep progression schema,that is precancerous lesions progress to adenocarcinoma in situ,and progress to micro-invasive adenocarcinoma,eventually some lung adenocarcinoma happen diffusion.However,recent findings reveal that this linear progression schema might not occur in all lung adenocarcinoma subtypes,and some subtypes show a kind of non-linear progression schema.The study of these two kinds of schemas can help for early detection of lung adenocarcinoma.