Objective To investigate serum levels of β-human chorionic gonadotrophin (β-HCG) and progesterone (P) in early pregnant women,and their relation to luteal maintenance therapy for early pregnancy protection.Methods One hundred and thirty five infertility women treated in Department of Gynecology and Obstetrics of Beijing Chaoyang Hospital from January 2007 to December 2011.Total 150pregnancy cycles,including 84 with intrauterine insemination (IUI) and 66 with natural conception,were divided into two groups:normal intrauterine pregnancy group (group A,n =80) and early pregnancy loss group (group B,n =70).Medical history,ultrasonic findings,serum female hormone,P and β-HCG levels at early pregnancy stage were analyzed.Results The age of group A and group B was (30.0 ± 3.9) years and(30.7 ± 4.9) years,respectively (P ＞ 0.05).The follicle-stimulating hormone/luteinizing hormone in group A and group B was 1.57 ±0.96 and 1.56 ± 1.08 ; the estradiol levels in two groups were (152 ±66) pmol/L and (147 ± 69) pmol/L,respectively (both P ＞ 0.05).There were no differences in dominant follicles and endometrial thickness between groups A and B (P ＞ 0.05).Ovulation promotion and luteal support treatments were adopted in both groups:50％ (40/80) of cycles in group A received ovulation promotion,73％ (58/80) of cycles received luteal support,while 44％ (31/70) and 76％ (53/70) received in groups B,respectively.The levels of serum progesterone in group A during 14-21 d,22-27 d and ≥ 28 d after ovulation were higher than those in group B at each time points (P ＜ 0.0l).There were no significant differences in serum progesterone levels between women with luteal support treatment and those without luteal support treatment in both groups (P ＞ 0.05).Conclusion Dynamic monitoring of serum progesterone level in early pregnancy may be used as an auxiliary index for prediction of pregnancy outcome,but it may not be an indicator for luteal maintenance therapy.

Trauma is one of the main reasons for the death and disability in children.Ultrasonic tech-nology has been applied in the early assessment and diagnosis of trauma.it is quick,effective,non-invasive, fast imaging,mobile and suitable for different patients and different parts of the body.It has been used to as-sess the organs in patients with severe trauma widely.

Gastrointestinal microbiota is a huge and complex system that has multiple influential factors. As an exogenous pathogen,Helicobacter pylori(Hp)may interact with gastrointestinal microbiota. It has been revealed that Hp infection led to an increase in Lactobacillus acidophilus in intestinal tract and a decrease in Actinobacteria,Bacteroidetes and Firmacutes in stomach. Proton pump inhibitors and antibiotics,the major components of eradication regimens for Hp infection,may also have an impact on gastrointestinal microbiota. On the other hand,gastrointestinal microbiota interferes with Hp’s attachment into gastric mucosa. Probiotics combined with eradication therapy could benefit the eradication rate of Hp infection and reduce adverse events.

0.05),but the above 3 groups were superior to FC group in the EIP incidence,pain degree and curative effect (P

Objective To study the expression of Sox2 in human prostate cancer and pericarcinomatous tissue,and the relation between its expression and the pathological type and clinical stage of prostate cancer.Methods Immunohistochemisty,Western blot and reverse transcription-polymerase chain reaction (RT-PCR)were used to detect the expression of Sox2 in 86 cases of prostate cancer tissues and 40 cases of pericarcinomatous tissues.The relation between Sox2 expression and the pathological type and clinical stage of prostate cancer was analyzed.Results The expression of Sox2 in prostate cancer tissue (71.9％) was significantly higher than that in pericarcinomatous tissue (7.5％),and the difference was statistically significant (x2 =53.98,P ＜ 0.05).Sox2 expression in prostate cancer tissues was significantly related to Gleason score (x2 =19.49,P ＜ 0.05).The positive rate of Sox2 in lymphatic metastasis group and negative lymphatic metastasis group was 92.3％ and 73.3％ respectively,and the difference was statistically significant (x2 =3.95,P ＜ 0.05).While there was no significant difference among clinical stages (x2 =0.16,P ＞ 0.05).Moreover,Western blot and RT-PCR revealed that the expression of Sox2 in prostate cancer tissue was significantly higher than that in pericarcinomatous tissue (t =-2.93,P ＜ 0.05 ; t =3.29,P ＜ 0.05).Conclusion The high expression of Sox2 is closely related to the Gleason score and lymphatic metastasis of prostate cancer,but not to the clinical stage.The overexpression of Sox2 may play an important role in formation,differentiation and metastasis of prostate cancer.

We produced α-ketoglutaric acid (α-KG) from L-glutamic acid, using enzymatic transformation approach with L-glutamate oxidase (LGOX). First, wild strain Streptomyces sp. FMME066 was mutated with NTG, a genetically stable mutant Streptomyces sp. FMME067 was obtained. Under the optimal nutrition conditions with fructose 10 g/L, peptone 7.5 g/L, KH2PO4 1 g/L and CaCl2 0.05 g/L, the maximum LGOX activity reached 0.14 U/mL. The LGOX was stable to pH and temperature, and Mn2+ had a stimulating effect. Finally, after 24 h enzymatic conversion under the optimal conditions, the maximum titer of α-KG reached 38.1 g/L from 47 g/L L-glutamic acid. Enzymatic transformation by LGOX is a potential approach for α-KG production.
Amino Acid Oxidoreductases ; metabolism ; Fermentation ; Glutamic Acid ; metabolism ; Ketoglutaric Acids ; metabolism ; Streptomyces ; genetics ; metabolism

Objective To study the role TGF-? 1 expression in the gastric cancer and to evaluate its clinical and pathological significance.Methods The expression of TGF-? 1 was studied by using S-P immunohistochemical technique in 20 cases of normal gastric tissue and 46 cases of gastric carcinoma.Results in the gastric cancer patients,the positive rate of TGF-? 1 expression was 100%,but there was 50% positive rate in the normal gastric tissues.The TGF-? 1 expression in group with lymph node metastasis was higher than that without lymph node metastasis.Conclusion TGF-? 1 plays an important role in the progress of gastric cancer.

Now the in-service staff have been permitted to apply for master's degree with the same educational level aspostgraduates. This is a signal reforming policy in postgraduate education and degrees management area in China. Manyhigher professionals are trained for our socialist modernization construction by this education technology. Although therelatively perfect system to manage this project was established in China, there are still some problems demandingprompt solution on the management thoughts, management process as well as management methods in some authorizedunits.

Oleic acid is a common pharmaceutical excipient that has been widely used in various dosage forms. Gas chromatography (GC) has often been used as the quantitation method for fatty acids normally requiring a derivatization step. The aim of this study was to develop a simple, robust, and derivatization-free GC method that is suitable for routine analysis of all the major components in oleic acid USP-NF (United States Pharmacopeia-National Formulary) material. A gas chromatography-flame ionization detection (GC-FID) method was developed for direct quantitative analysis of oleic acid and related fatty acids in oleic acid USP-NF material. Fifteen fatty acids were separated using a DB-FFAP (nitroterephthalic acid modified polyethylene glycol) capillary GC column (30 m × 0.32 mm i.d.) with a total run time of 20 min. The method was validated in terms of specificity, linearity, precision, accuracy, sensitivity, and robustness. The method can be routinely used for the purpose of oleic acid USP-NF material analysis.

Objective To investigate prognosis of endometriosis and to determined correlative factors of pelvic pain associated with endometriosis after conservative surgery.Methods The clinical data in patients with pelvic pain related to endometriosis and underwent surgery treatment from January 2000 to September 2009 in Beijing Chaoyang Hospital were collected and sorted,then the patients were followed up.The data were analyzed by statistical software SPSS 13.0 for windows.Results A total of 332 patients with endometriomas were underwent conservative surgery,281 (84.6％,281/332) cases of them relieved pelvic pain.The univariate analysis showed that the effects were signficantly with age,Douglas pouch reconstruction,postoperative medical therapy,presacral neurectomy (PSN) and combined adenomyosis (all P＜0.05).The multivariate analysis also showed that age(OR=0.946,P＜0.01),Douglas pouch reconstruction (OR=0.208,P＜0.01),postoperative medical therapy (OR=0.504,P＜0.01),PSN (OR=0.121,P＜0.05) and combined with adenomyosis (OR=2.528,P＜0.01) were also the correlative factors to prognosis.Conclusion The protective factors for prognosis of pelvic pain associated with endometriosis included age,Douglas pouch reconstruction,postoperative medical therapy and PSN,while the risk factor was combined with uterine adenomyosis.