ObjectiveThe relationship between clinicopathological features and prognosis in the women patients with breast mucinous carcinoma was investigated. Methods 101 patients with breast mucinous carcinoma were divided into two groups:pure mucinous carcinoma ( n =74), and mixed type ( n =27). The relationship between clinicopathological features and prognosis was analysed. Results Lymph node was less frequently involved in pure type group (24%) than that in the mixed type (52%) (? 2 =10 4, P

Objective To evaluate the efficacy of endoscopic techniques for corrections of facial contour deformities.MethodsA total of 58 patients(48 women and 10 men)with square face(38),prominent zygoma(12),or crooked noses(8)were treated by endoscopic-assisted technique in our hospital.Results The operation time ranged from 40 to 86 min(mean,65 min),and intraoperative blood loss was 60-120 ml(mean,78 ml).The patients were discharged from the hospital in 3 to 7 days(mean 4)after the surgery,and was followed up for 3 to 24 months.During the period,no one had unexpected fracture,injuries to the mental and facial nerves and vessels,or infection.All the patients were satisfied with the surgical outcomes.ConclusionsEndoscopic-assisted correction of facial contour deformities is feasible,safe,and effective.

Objective To explore the association between gene polymorphism of interleukin(IL)-4 C590T and serum level of IL-4 and atherothrombotic cerebral infarction(ATCI).Methods The gene polymorphisms of IL-4 C590T in 159 patients with ATCI and 151 normal control subjects were carried out by polymerase chain reaction(PCR)-restriction fragment length polymorphism(RFLP).The serum level of IL-4 in normal control group was determined with enzyme linked immunosorbent assay(ELISA) method.Results In the normal control group,the serum level of IL-4 in TT genotype[(31.85?5.68) pg/ml] was higher significantly than those in TC[(28.67?6.00) pg/ml] and CC genotypes[(20.98?0.99) pg/ml],TC genotype was higher significantly than CC genotype(all P0.05).Conclusions IL-4 590T allele is associated with higher level of serum IL-4.The C590T polymorphism is not associated with the incidence of ATCI.

Objectives: To explore the infulence of early enteral nutrition on nutritional and immunal status and complications for elderly patients with gastric neoplasm. Methods: 84 cases of gastric neoplasm were randomly divided into TPN group( n =42),an EN group ( n =42).Equal calorie and nitrogen were given from the frist day after operation. Nutritional and immune markers were examined before and after nutrition support. The complication and gastrointestinal function were carefully observed . Results: Nutritional markers and immune markers were significantly increased after nutritional support both in TPN and EN group ( P 0.05). EN can promote resumption of gastrointestinal function,decrease complications and reduce the medical cost. Conclusions: Early post operative enteral nutrition for elderly patients with gastric neoplasm is a safe,effective,economic nutritional treatment.

Objective To search for a procedure for correction of square face. Methods 68 patients with square face (aged from 21～40 years, with 65 females and 3 males) underwent osteetomy combined mandibular angle triangle resection with lateral cortex around the mandibular angle-splitting by intraoral approach. The mandible outer cortex was splited with bone chisel and mandible angle was resected by the high-speed pneumatic drill. The bulky masseter muscle and the enbonpoint buecal fat-pad were scissored. Results The application of high-speed pneumatic drill and bone chisel facilitated resection of the mandibular angle with lateral cortex splitting osteetomy. The operative effects were satisfactory in 56 patients during 3-24 months of follow-up. The bigonial distance was effectively re-duced, and facial shape became ellipse. The gross asymmetry and mandibular fracture were not conse-quences. The lateral contour of the mandibular angle was natural. Conclusion Ostectomy of mandible angle and lateral cortex of the mandible allows the reduction of the mandibular contour of the frontal view and the lateral contour of mandibular angle becomes soft, giving a more natural appearance. This new method is worthy to be applied widely because of the good result with effective reduction of the mandibular width.

OBJECTIVE: To investigate the characteristics of air-conducted ocular vestibular-evoked myogenic potential(oVEMP) in patients with sudden sensorineural hearing loss (SSHL). So as to understanding the range and the degree of the vestibular system damage in patients with SSHL. METHOD: Thirty-five cases of unilateral SSHL were enrolled as the observing group from December in 2013 to December in 2014. 500 Hz air-conducted tone burst was employed as stimulation. Fifity-five healthy young subjects were recruited as the control group. The results were compared between the affected ears, the contralateral ears and the normal controls. RESULT: Thirty-five patients with SSHL, 31 of them in both ears lead to oVEMP Waveform, 4 sick ears did not elicit oVEMP waveform, extraction rate of 88%. 95 dBHL air-conducted get the latencies of P1 and N1, P1-N1 interval, peak-to-peak P1-N1 amplitude in healthy ear were (11.92 ± 0.85)ms; (17.07 ± 1.04)ms, (5.15 ± 0.69)ms, (5.44 ± 2.53) µv. 95 dBHL air-conducted get the latencies of P1 and N1, P1-N1 interval, peak-to-peak P1-N1 amplitude in sick ear were (12.16 ± 0.76) ms; (16.94 ± 2.57)ms, (5.16 ± 0.73)ms, (2.89 ± 1.66) v. The 55 cases (110 ears) were elicited P1-N1 complex typical, extraction rate was 100%. The threshold of oVEMP examination was (82.23 ± 2.92) dBHL, 95 dBHL air-conducted get the latencies of P1 and N1, P1-N1 interval, peak-to-peak P1-N1 amplitude in these healthy young people were (11.53 ± 0.80)ms, (16.61 ± 0.87) ms, (5.18 ± 1.04) ms, (5.96 ± 2.59) µv, there were no significant differences between the affected ears, the contralateral ears and the normal controls in the latencies P1 and N1, P1-N1 interval. The wave amplitude in SSHL were lower than the healthy ear and the healthy control group. CONCLUSION Through the detection of oVEMP in patients with SSHL,we observed that P1-N1 wave amplitude decreased, and it may be related to the damage of the utricle and vestibular nerve.
Case-Control Studies ; Hearing Loss, Sensorineural ; physiopathology ; Humans ; Saccule and Utricle ; pathology ; Vestibular Evoked Myogenic Potentials ; Vestibular Nerve ; pathology ; Vestibule, Labyrinth ; physiopathology

ObjectiveTo evaluate the clinical and functional outcome of traumatic segmental defects of the tibia treated by internal bone transport using the the adjustable mono-lateral external fixation.Methods12 patients who underwent internal bone transport using the the adjustable mono-lateral external fixation for traumatic segmental defects in the tibia were reviewed, the length of bone defect was 6～10 cm.ResultsMean follow-up was 14 months. All the cases got primary bone union. The length of time in healing was 9～14 months.ConclusionTraumatic segmental defects of the tibia treated by internal bone transport using the adjustable mono-lateral external fixation is a simple, less traumatic, non-bone graft and effective way.

Objective To evaluate the value of the clinical application of endoscopieally assisted re-duction malarplasty. Methods A total of 12 women (aged 21 -36 years) with prominent zygoma were re-shaped by the endoscope-assisted technique. Results The use of an endoscope offered a clear view of the operating field and facilitated accurate and easy manipulation. These patients were successfully treated for this new technique: reduced risk of operating injuries. Conclusion It is showed that the endoscopic assis-ted correction of prominent zygoma offers more satisfactory clinical results with a clear view of operation and a minimally invasive technique.

AIM: To study the expression of hepatitis B virus core gene in Pichia pastoris and to obtain high-level expressed recombinant HBcAg with good immunoreactivity and high specificity. METHODS: HBV core gene was amplified by PCR from plasmid pHBV1 which contained HBV whole DNA sequence. The PCR product was cloned into pGEM-T vector by TA cloning strategy. After confirmed by DNA sequence analysis, the gene of interest was inserted into the yeast expression vector pPIC9. The recombinant plasmid pPIC9-cAg was constructed and transformed into GS115 by electroporation. The recombinant yeast GS115 was induced by 0.5% methanol. The expressed product was analysed by SDS-PAGE,Western blot and ELISA. RESULTS: The restriction analysis and DNA sequence analysis proved that HBV core gene had already been cloned to yeast expression plasmid pPIC9. The expressed HBcAg existed in SDS-PAGE. Good immunoreactivity and high specificity of the recombinant HBcAg have been proved by ELISA and Western blot. The titre of the recombinant HBcAg in the cell lysate was 1∶ 12 800 . CONCLUSION: The recombinant plasmid pPIC9-cAg was successfully constructed. The recombinant HBcAg with good immunoreactivity and high specificity was successfully expressed in Pichia pastoris expression system and can be applied to further developing HBcAb immunoassay. [

Objective To transfect PCDNA3.0-HIF1-? into the endothelial progenitor cells(EPCs),in order to provide the foundation for exploring myocardial vascular repairing capacity and angiogenesis of the EPCs transfected with HIF gene.Methods The EPCs were separated by the method of density gradient separation in the human bone marrow,and then the cells were inoculated in the fibronectin-packaged petri dishes complete medium with the concentration of 1?106?mL-1,and cultivated for 14 d. The expressions of the endothelial cell-specific components ecNOS and flk-1 were detected with RT-PCR; the cells were stained with acLDL-Dil and FITC-UEA-1; the PCDNA3.0-HIF1-? plasmid was transfected into the EPCs mediated by LipofectamineTM 2000,the transcription of HIF1-? was determined with RT-PCR; the expression of VEGF was detected with enzyme-linked immunosorbent assay (ELISA); the expression of HIF1-? was detected with Western blotting.Results After cultivated for 5 d,some round monocytes transformated into spindled inherent EPCs;7 d later,there were some cell colonies containing dozens of cells;10 d later,there were obvious cell clones; RT-PCR showed ecNOS band in 548 bp,flk-1 band in 819 bp; acLDL-Dil and FITC-UEA-1 double-staining was positive; RT-PCR showed specific band in 383 bp; ELISA showed the content of VEGF in the supernatant of EPCs transfected with HIF1-? was (20.53?2.33) ?g?L-1,and it was (3.96?1.67) ?g?L-1 in the EPCs transfected without HIF1-?,there was significant difference between two groups (P