Objective To evaluate the clinical effect of neuromatosis scoliosis treated by vascularized grafted rib Method The clinical date of 16 cases of neuromatosis scoliosis treated by surgical operation were analysed retrospectively Result Sixteen patients with scolisis caused by neurofibromatosis were treated by this technique The clinical result was satisfactory and solid fusion was observed in all patients within 6 months after operation Conclusion This operation is an effective method for the treatment of neuromatosis scoliosis

OBJECTIVE: To investigate the preventive effect of Antine Capsule on the pain(EIP) in patients receiving root canal therapy.METHODS: The medication group(n=60) were assigned to receive Antine Capsule for 3 days after root canal preparation,while the Control group(n=69) to receive routine therapy.The incidence and degree of time-dependent EIP within 7 days after operation were observed.RESULTS: The EIP incidence rate and the degree of pain in the medication group were significant lower than in routine RCT group(P

To evaluate the effects of silicone points polishing on plaque index(PI) and sulcus bleeding index(SBI) after ultrasonic scaling. Forty patients with periodontal diseases were selected for ultrasonic scaling. After scaling,unilateral teeth were randomly assigned for silicone points polishing. PI and SBI were used to evaluate the effects of silicone points polishing. Silicone points polishing significantly reduced PI 12 weeks after scaling. Both in the polishing group and the control group, SBI decreased after scaling. The SBI in the polishing group was significantly lower than that in the control group in the 24 weeks. Silicone points polishing can effectively increase the smoothness of tooth and enhance the effects of uhrosonic scaling.

OBJECTIVE:To evaluate the clinical efficacy of Calcium Hydroxide Iodoform Glycerin(CHIG)paste for root canal disinfection.METHODS:145 patients(145 teeth)requiring root canal therapy were divided randomly into CHIG group,formo-cresol solution group(Group B)and camphor phenol group(Group C).After root canal preparation,the corresponding drugs were introduced into root canal and pulp chamber.The endodontic interappointment pain(EIP)and clinical efficacy in the 3 groups were compared.RESULTS:In Group A,the EIP incidence was the lowest(8.16%)while the effective rate was the highest(97.96%),showing significant differences as compared with Group B(31.91% and 68.09%,respectively)or Group C(34.69% and 71.43%,respectively)(P

Objective To study the role TGF-? 1 expression in the gastric cancer and to evaluate its clinical and pathological significance.Methods The expression of TGF-? 1 was studied by using S-P immunohistochemical technique in 20 cases of normal gastric tissue and 46 cases of gastric carcinoma.Results in the gastric cancer patients,the positive rate of TGF-? 1 expression was 100%,but there was 50% positive rate in the normal gastric tissues.The TGF-? 1 expression in group with lymph node metastasis was higher than that without lymph node metastasis.Conclusion TGF-? 1 plays an important role in the progress of gastric cancer.

Objective To examine the surgical treatments for obstructive jaundice caused by bile duct strictures after hepat-ic transcatheter arterial chemoembolization(TACE).Methods Clinical data were retrospectively analyzed of 9 patients who de-veloped bile duct strictures among 229 patients after TACE between June 1994 and March 2011 in People’s Hospital of Zhe-jiang Provincial and the Second Affiliated Hospital of Zhejiang University.There were 6 cases of primary liver cancer,and 3 ca-ses of metastatic liver cancer.Obstructive jaundice occurred 5 to 16 months after TACE treatment.The median time was 8 months.Results All the nine patients with bile duct strictures suffered different degrees of obstructive j aundice,which was cured after surgical operations or PTC + stenting in 7 patients and significantly relieved by percutaneous transhepatic cholangic drainage(PTCD)in 2 ones.Two patients with obstructive j aundice after TACE for primary liver cancer were followed up for 3 years,and no recurrence of hepatic carcinoma and bile duct obstruction was found.The other 7 patients were followed up for 3 to 13 months,and they all died of deterioration of primary disease.Conclusion Patients with obstructive jaundice caused by bile duct strictures after TACE benefit from surgical operations or intervention treatment.Different surgical strategies are selected based on the characteristics of primary disease,the site and extent of bile duct stricture.

Objective To explore the effects of salmeterol/fluticasone propionate complicated or combined with N-acetylcysteine on the pulmonary function and arterial blood gas analysis of the patients with chronic obstructive pulmonary disease (COPD), and to evaluate its curative effect.Methods 84 cases of COPD patients were randomly divided into combination treatment group (n=44)and simple treatment group (n=40).The patients in combination treatment group were treated with salmeterol/fluticasone propionate combinated with N-acetylcysteine while the patients in simple treatment group were treated only with salmeterol/fluticasone propionate, both of which were followed up for 6 months.The changes of pulmonary function (FEV1%FVC,FEV1%Pred,PEF daily variation rate:ΔPEF%)and the arterial blood gas analysis indexes (PaO2 and PaCO2 )of the patients in two groups were recorded before treatment, 3 months after treatment, and 6 months after treatment. Results The FEV1%FVC,FEV1%Pred and PaO2 of the patients in combination treatment group and simple treatment group were obviously increased 3 and 6 months after treatment compared with before treatment (P<0.01 ), and the PaCO2 were obviously decreased (P<0.01).And there were significant differences of the indexes mentioned above of the patients between two groups (P<0.05 );but the indexes of each group had no significant differences between 3 months and 6 months after treatment (P>0.05).TheΔPEF% of the patients in two groups had no significant differences between inter-group and intra-group before and after treatment (P > 0.05 ). Conclusion Combination of salmeterol/fluticasone propionate and N-acetylcysteine can obviously improve the pulmonary function and arterial blood gas analysis indexes of the COPD patients, which is superior to the simple application of salmeterol/fluticasone propionate and has definite and lasting curative effect on the treatment of COPD.

Objective To investigate the mechanism of radiosensitizing effects of endostatin on H-520 human lung squamous cancer cells.Methods H-520 cells was treated with endostatin and/or radiation.Colony-forming assays were used to indicate the radiosensitising effects.Cell cycle distribution and expression of phosphor-p38-MAPK were assayed by FCM,and cyclin D1,cdk2,cdk4 and survivin mRNA leveh were assayed by RT-PCR.Phosphor-Akt was evaluated by Western-blotting.Results Combination of endostatin and irradiation inhibited the proliferation of H-520 cells.According to the colony-forming assays,the D0,Dq,D10 and SF2 values of the combination groups were much lower than those of irradiation groups.The sensitization enhancement ratio(SER)was 1.51.G2/M arrest occurred after 4 Gy irradiation.The gene expression of cyclin D1,cdk2,ckd4 and survivin and phosphor-Akt protein were down-regulated after treatment.The expression of phosphor-p38-MAPK protein was also down-regulated after treatment with 200 μg/ml endostar.Conclusions Endostatin inhibits the growth of H-520 cells and radiosensitizes the cells by induction of G0/G1 arrest,cell apoptosis and down-regulation of gene expression of cyelin D1,cdk2,cdk4 and reduces the phosphorylation of Akt and p38-MAPK.

Objective To discuss the feasibility and effect of labeled antibody on targeting therapy of systemic lupus erythematosus(SLE) associated thrombocytopenia.Methods MTX-IVIG conjugate was prepared by indirect and direct cross linking,and the binding ability of it to Fc fragment was detected by indirect immunofluor-escence.The killing activity of the conjugate was detected by MTT method using murine macrophage with Fc receptor and strain U937 of human monocytic leukemia cell as targets.Results Conjugation showed stronger cytotoxicity upon target cells than free MTX,and it showed less cytotoxic effect on Fc receptor negative cells compared with the positive ones.IVIG-HSA-MTX restrained the phagocyte of macrophage.The killing effect of IVIG-HSA-MTX was significantly stronger than that of IVIG-MTX.Conclusion The conjugation can show a highly specific cytotoxicity upon mononuclear-macrophage in vitro.

Objective To evaluate the effect of basic fibroblast growth factor(bFGF) on sheep bone marrow mesenchymal stem cells(BMSCs) proliferation and differentiation in vitro.Methods The sheep BMSCs were isolated and expanded in vitro and divided into experimental group and control group.bFGF(2 ?g?L-1) was added into cell culture medium of experimental group.In control group,no bFGF was added.The morphological differences of BMSCs between two group were observed.Growth curves and alkaline phosphatase(ALP) activity were assessed.In order to observe the influence of bFGF on differentiation potential of BMSCs,osteogenic and adipogenic inductions were conducted on BMSCs in two groups.Results Fibroblast colonies appeared 1 week after seeding of the mononuclear cells.2 weeks later,more colonies formed and each colony became larger.The cell number was 2.5 times of the control when the first passage cells grew to confluence and the ALP activity in experimental group was lower than that in control group(P