1.Evidence-based medicine and treatment of lung cancer
Journal of Integrative Medicine 2003;1(2):151-4
Traditional Chinese medicine (TCM) thinks highly of Zheng Hou in the treatment of lung cancer. Zheng is evidence. Hou is clinical manifestations. After obtaining information and evidence of patients by means of the four diagnostic methods, doctors treat patients by syndrome differentiation. In TCM, the collection and study of medical literature of tumor are emphasized. Medical classics and successful treatment of certain cases are used to guide clinical treatment. Such practice of TCM is similar to that of evidence-based medicine (EBM). Oncology of TCM and EBM are all of ancient origin. There are many clinical reports of satisfactory curative effects in treating lung cancer by TCM. But there are still many problems in methodology, such as careless random method, incomparability in base line, and deficiency of large exponent, multicenter, blind method or randomization. Sometimes the effects of control drugs are incredible. The determination of near effect is not strict. There are exponent errors in statistics of near effect(CR+PR). Certainly, treatment of lung cancer by TCM has its uniqueness. The main characteristic of treatment by TCM is that it can restrain or slow the growth of tumor. At the same time, physical signs, symptoms and quality of life can be improved, and survival period is prolonged. "Survival rate is the golden standard". If we can explore a new treatment strategy to control the growth and metastasis of advanced NSCLC and prevent relapse and metastasis of earlier period or metaphase NSCLC by the method of binding TCM and west medicine under the rules of EBM, such as multicenter (TCM hospital and western medicine hospital), blind method, strict randomization and control, the conclusion of the study will be more scientific.
2.Effect of NK-104 on NF-?B activated by TNF-? in hepatocellular carcinoma cells
China Oncology 1998;0(04):-
Background and purpose:A causal relationship between inflammation and cancer has been extensively investigated. Nuclear factor-kappa B (NK-?B) is a key regulator in inflammation-associated cancer, and is activated by a variety of stimuli. This paper studied the anti-inflammatory effect of NK-104 on NF-?B activated by TNF-? in hepatocellular carcinoma cell line (Huh 7). Methods:The cell proliferation was examined by the WST-8 assay. The NF-?B and I?B? expressions were evaluated by western blotting. IL-8 and its product were quantitated by ELISA.Results:TNF-?(1 ng/ml) strongly induced the expression of NF-?B by approximately 1.8-fold compared with the control in the nuclei of Huh 7 cells, the induced NF-?B expression was significantly suppressed by the addition of pitavastatin at 0.1 ?mol/L (P 0.05). Subsequently, the addition of TNF-? significantly increased IL-8 production, which could be reversed by the addition of NK-104.Conclusions:These results suggest that NK-104 at low dose (0.1 ?mol/L) inhibits NF-?B activation and decreases IL-8 production induced by TNF-?. It is therefore expected to play a role in the new strategy for the treatment of hepatocellular carcinoma.
3.Effects of NK-104 on apoptosis and caspase-3 activity in hepatocellular carcinoma cells.
Journal of Integrative Medicine 2007;5(3):298-301
To study the apoptosis of human hepatocellular carcinoma cell line HepG 2 induced by pitavastatin (NK-104) and to investigate the change of caspase-3 activity.
4.Gene polymorphism and physical constitution theory: starting point of exploring syndromes of lung cancer.
Journal of Integrative Medicine 2010;8(1):15-9
Physical constitution theory is an important part of traditional Chinese medicine theory. Physical constitution is associated with the incidence, development and prognosis of diseases. Gene polymorphism is a result of the interaction between internal and external environments during the human evolution, and it is the reason for differences in biological characteristics and disease susceptibility of individuals. Current status of the research on physical constitution theory, lung cancer syndromes and gene polymorphism are summarized in this paper. Exploring lung cancer syndromes from the point of view of gene polymorphism may reveal the scientific connotation of lung cancer syndromes, and provide new evidence and method for diagnosis of lung cancer.
5.An optimized 3-step strategy for preventing and treating post-operation relapse and metastasis of malignant tumors with traditional Chinese medicine
Journal of Integrative Medicine 2007;5(1):5-10
Abstract: Relapse and metastasis are the main causes responsible for failure of surgical operation in treating malignant tumors. This article introduces the present development in post-operation treatment of lung cancer, liver cancer and stomach cancer, showing that the synthetic treatment by stages combined with traditional Chinese medicine is the important way to prevent and treat the post-operation relapse and metastasis of tumors, and suggesting the optimized method for treatment.
8.Study on the Influence of Feiyanning Decoction on Expression of E-cadherin,?-catenin and ?-catenin of Mice with Metastatic Lung Cancer
Xiaozhen ZHAO ; Zhenye XU ; Zhonghua WU
Journal of Traditional Chinese Medicine 1992;0(09):-
Objective To study the expressions of epithelial symbol factors including E-cadherin,?-catenin,and ?-catenin related to epithelial-mesenchymal transition(EMT) in C57 mice Lewis lung cancer and its mRNA expression affected by Feiyanning Decoction(Decoction for lung cancer).Methods The Real-Time PCR method was adopted to observe the E-cadherin,?-catenin,?-catenin mRNA expression of C57 mice with transplanted tumor in the right armpit and distal metastases and the affection of Feiyanning Decoction on the expression.Results The lung transplanted rate in the Feiyanning group was clearly lower than that in the model group(P
9.Shuanghuang Shengbai Granule on the mechanism of leukogenic effect
Yunhui SHEN ; Changxun CHEN ; Zhenye XU
Chinese Traditional Patent Medicine 1992;0(04):-
AIM: To observe the effect of Shuanghuang Shengbai Granule (Radix astragli, Rhizoma polygonati, Fructus ligustri lucidi, Radix trichosanthis, Rhizoma drynariae, etc) (SHSB) on the proliferation of bone marrow cells and the growth of granulocyte-macrophage colony stimulating factor(GM-CSF) in leukopenia model mice, and to investigate the related mechanism. METHODS: Mouse leukopenia models were made by radiation, the proliferation of bone marrow cells was determined by MTT chromatoptometry, the content of GM-CSF in serum was measured by double antibody sandwich ELISA, and the protein expression of GM-CSF in cultured spleen cells was measured by SP immunocytochemistry. RESULTS: SHSB could promote the proliferation of bone marrow cells; increase the content of GM-CSF in serum and enhance the protein expression of GM-CSF in spleen cells. CONCLUSION: SHSB Granule has the improvement in enhancing the proliferation of bone marrow cells in leucopenia model mice, increasing the content of GM-CSF in serum and the protein expression of GM-CSF in spleen cells.
10.Effects of Xuanyinning Recipe on invasion of SPC-A-1 cells and pathomorphological changes of peritoneum in mice inoculated with sarcoma 180
Zhenye XU ; Jianwen LIU ; Meiyun ZHOU
Journal of Integrative Medicine 2005;3(4):282-5
OBJECTIVE: To observe the effects of Xuanyinning Recipe (XYNR) in inhibiting SPC-A-1 cellular infiltration and on the pathomorphological changes of peritoneum in mice inoculated with sarcoma 180 (S(180)). METHODS: On the bases of isolated culture of mouse peritoneal mesothelial cells, we adjusted and added the human lung adenocarcinoma cell line SPC-A-1 into the double-layer culture medium to observe the number of clones formed. We also took out the peritoneum from the mice administered with three different dosages of XYNR and observed its pathomorphological changes with transmission electron microscope. RESULTS: In the in vitro experiment, the number of clones of SPC-A-1 in culture medium with XYNR (50 microg/ml) decreased distinctly. In the in vivo experiment, it was observed that, in the peritoneum from the XYNR-treated mice inoculated with S(180), the mesothelial cells arranged more and more regularly with the increasing of the dosage of XYNR, while the mesothelial cells in the peritoneum of the mice in the control group necrosed and arranged loosely. CONCLUSION: XYNR can inhibit the invasion of SPC-A-1 cells. It also can improve the loose arrangement of the peritoneal mesothelial cells in mice inoculated with S(180), so as to inhibit the malignant effusion.