1.The management of cerebrospinal fluid leakage after transsphenoidal surgical removal of pituitary adenomas
Chinese Journal of Postgraduates of Medicine 2009;32(5):39-41
Objective To evaluate the incidence and the causes of the intra-and postoperative cerebrospinal fluid(CSF)leakage during transsphenoidal surgical removal of pituitary adenomas,and discuss the sella closure methods.Method During the period from January 1998 to May 2008,118 patients underwent 134 transsphenoidal operations for pituitary adenomas.Two different methods to close the sella were used.The first one consisted packing the sella turcica and sphenoidal sinus with autologous fat and restoring the defect of sella turcica with autologous bone.In the second method,tht regenerated oxidized cellulose and collagen sponge with human fibrin were used to cover the seUa membrane defect and the dural defect,followed by packing the seHa with autologous fat.Proportions of each type of pituitary adenomas were analyzed.The frequency of the intra-and postoperative CSF leakage were evaluated in different type tumors and different operations.Data had been studied by statistical analysis.Results The incidence rate of intraoperative CSF leakage(26.5%,9/34)Was obviously increased in patients with growth homone adenoma compared with other type of adenoma(13.0%,13/100).Patients with invasive adenoma had an increased incidence rate of intraoperative CSF leakage(21.1%,8/38)compared with noninvasive adenoma(14.6%,14/96)(P<0.05).When the descent of the sella turcica membrane Was visualized with tumor being totally removed,the incidence rate of intraoperative CSF leakage increased(18.7%,20/107)compared with when membrane descent was not seen with tumor remaining(7.4%,2/27)(P<0.05).There were 3 cases postoperative CSF leakage happened in all 12 cases which adopted the first method. By the second method,there was no postoperative CSF leakage,and the number of postoperative complications was significantly smaller than the first method (10.0% and 33.3%,respectively). Conclusions The technique of covering the sella membrane and dural defects with oxidized cellulose and collagen sponge with human fibrin in the presence of intraoperative CSF leakage appears to be the most reliable,as no postoperative CSF leakage after applying this technique has been observed.
2.Ancient Greek Medicine and Ancient Chinese Medicine Division from the Relationgship between Medicine and Philosophy:Taking the Hippocratic Corpus and the Inner Canon of Yellow Emperor for Example
Chinese Medical Ethics 2015;(3):407-411
Unlike modern large differences between Chinese and Western medicine , in the early stage of hu-man development , the ancient Greek medicine and ancient Chinese medicine during their exploration has a remark-ably consistent .They all represent the most wisdom philosophy , and good tradition a doctor should have , and thus formed the medical ethics that affect later generations .We can see segment of a whole from the theoretical system between the earliest Chinese medical Inner Canon of Yellow Emperor which formed in the spring and autumn period and the warring states period and Hippocratic Corpus which is on behalf of the 5th century BC to the 2nd century BC, the ancient Greek medicine highest achievement .But since then , Chinese traditional medicine and Western medicine have achieved the last collision , And around the second century AD completely broke ranks .We have to seek the origins from the two kinds of heterogeneous soil China and ancient Greece .Research of the relationship be-tween the period of medicine and philosophy can help us find beneficial enlightenment for the difference .
3.ROLE OF CASPASE-1 ACTIVATED CYTOKINES IN ACUTE LUNG INJURY IN EXPERIMENTAL SEVERE ACUTE PANCREATITIS
Xiaohua ZHANG ; Zhaoshen LI ; Zhenxing TU
Medical Journal of Chinese People's Liberation Army 2001;0(12):-
Objective To study the role of Caspase-1 activated cytokines in acute lung injury (ALI) in experimental severe acute pancreatitis (SAP). Methods A rat model of SAP was induced by retrograde infusion of 5% sodium taurocholate into the biliary pancreatic duct. Thirty-two SD rats were randomly divided into four groups: healthy control (HC), SAP 6h, SAP 12h and SAP 18h groups. Serum IL-1? level was measured by ELISA. Intrapulmonary expressions of Caspase-1, IL-1? and IL-18 mRNA were detected by semi-quantitative RT-PCR. Results The serum IL-1? levels were significantly increased after the induction of pancreatitis (P
4.Liver injury of acute pancreatitis:analysis of 214 cases
Guifang XU ; Weijie ZHANG ; Zhenxing TU
Chinese Journal of Practical Internal Medicine 2001;0(09):-
Objective To study clinical characteristics and prognosis of patients with liver injury of acute pancreatitis.Methods 290 cases of acute pancreatitis admitted between January 2001 to October 2003 were reviewed and analyzed retrospectively.Results Through comparing different types of AP with liver injury and C-reactive protein (CRP) changes,it was found that the more severe AP was,the more significant liver injury was;and liver injury of severe AP had some connection with CRP(.P.
5.Clinical characteristics of severe pneumonia in children and the significance of neutrophil elastase detection
Hui CHEN ; Zhenxing ZHANG ; Chao LU
The Journal of Practical Medicine 2014;(4):554-556
Objective To study the clinical characteristics of severe pneumonia in children , to explore the significance of neutrophil elastase detection. Methods Patients were divided to severe pneumonia group and non-severe pneumonia group Fever, cough, lung wet rale duration and image changes were statistically analyzed. The percentage of abnormal lymphocyte in peripheral blood was counted by Wright's dyeing method. The neutrophil elastase expression in peripheral blood was determinated by enzyme-linked immunosorbent analysis. Results In severe pneumonia group,atelectasis occurred in 5 cases,8 cases complicated with heart failure, 8 cases complicated with respiratory failure and 4 cases were complicated with toxic encephalopathy. In severe pneumonia group , fever time was 13.5±5.1 days, cough time was 15.1±3.2 days, the time of lung wet rale duration was 12.2±2.3 days, which were significantly longer than that of non-severe pneumonia group (t = 2.346,2.457,2.346,P < 0.05);In severe pneumonia group , pathogens included streptococcus pneumoniae , respiratory syncytial virus and adenovirus , etc.In severe pneumonia group,the percentage of abnormal lymphocyte in peripheral blood smear increased with virus infection (8.1±1.2)%. In 12 cases of severe pneumonia,the expressions of neutrophil elastase were 127.6± 12.5 ng/ml, more than that of non-severe pneumonia group (75.4 ± 6.4 ng/ml,t=3.047, P<0.05). Conclusion Severe pneumonia is a serious diseases impacting children health. Detection of neutrophil elastase shows a markedly clinical value to judge the severity of pneumonia.
6.On the Relationship between Improving Physician-Patient Morality Accomplishment and Constructing a Harmonious Society
Gang LV ; Zhijie WANG ; Hui ZHANG ; Zhenxing LI
Chinese Medical Ethics 1995;0(02):-
To improve physician-patient morality accomplishment is the basic project for construct a harmonious society,a harmonious physician-patient relationship,and a harmonious hospital,thus is of great realistic significance and value.The improvement of physician-patient morality accomplishment contributes to the construction of a harmonious society,while a harmonious society also lays a solid foundation for the improvement of physician-patient morality accomplishment.Thus the two depend on,contribute and interact with each other.
7.Establishment of an orthotopic transplantation model of osteosarcoma labeled with three reporter genes
Shanfeng ZHANG ; Zhenxing LIU ; Zhiwei YE ; Zhonghua YANG
Military Medical Sciences 2015;(10):755-758
Objective To establish an orthotopic osteosarcoma nude mice model that co-expresses green fluorescent protein( GFP) , red fluorescent protein ( RFP) and luciferase for the purpose of monitoring the growth of osteosarcoma and screening drug candidates against osteosarcoma .Methods Human osteosarcoma cells of U 2-OS were infected with lentivirus carrying reporter gene .The reporter gene expression was verified by fluorescent microscopy and bioluminescence imaging.The cells were transplanted into tibia of the nude mice and monitored by bioluminescence imaging .Results The reporter gene was stably expressed in U 2-OS cells.The growth and metastsis of osteosarcoma could be detected in nude mice.Conclusion The established orthotopic osteosarcoma nude mice model is an ideal model for investigating the mechanism of growth and metastasis of osteosarcoma and for screening drug candidates against osteosarcoma .
8.Development of WMZ-03 anaesthesia workstation with chest intergrated with apparatus
Taihu WU ; Zhenxing SONG ; Zhen WAN ; Xiaofeng ZHANG
Chinese Medical Equipment Journal 1989;0(03):-
This paper introduces a type of anaesthesia workstation which is composed of two vertical medical chests, i.e. the upper and lower parts. Taken out of the chest, the two parts are butted against and locked to make the workstation ready for operation. After operation is finished, the two parts are uncoupled and put into the chests, and then the workstation can be stored and transported with the chests closed.
9.Expression of novel apoptosis-related protein PDCD5 in granulosa cells of polysystic ovary syndrome
Chunling SUN ; Jie QIAO ; Zhenxing HU ; Ting ZHANG ; Yingyu CHEN
Journal of Peking University(Health Sciences) 2003;0(05):-
Objective: To detect the novel apoptosis-related protein PDCD5 expression in granulosa cells of polysystic ovary syndrome(PCOS) and normal ovary, and explore the pathogenesis of PCOS. Methods:The granulosa cells were collected from 30 cases of PCOS and normal ovary in IVF-ET. Expression of PDCD5 was detected by flow cytometry; immunofluorescence and immunohistochemistry. Cell apoptosis was detected by Propidium Iodide (PI) staining. Results: The number of hypodiploidy cells associated with apoptosis in granulosa cells of PCOS was greater than that of the normal control. PDCD5 protein expression in PCOS granulosa cells was significantly higher than that in normal ovary(P
10.Study on anti-shock trousers
Taihu WU ; Zhenxing SONG ; Wei WEI ; Xiaofeng ZHANG ; Shutian GAO
Chinese Medical Equipment Journal 1989;0(04):-
This paper introduces a novel trousers,whose theory is to drive blood by inflated pressure.Being light-weight,small and effective,easy to carry,store and operate,it is postured for first aid and resuscitation of hospital patients with severe shock,and also can be applied to bleeding remission,hemorrhagic as well as fracture immobilization.