Objective:To investigate the the effect of CD151 on Akt-Ser473 and human umbilical veine endothelial cell proliferation.Methods:To construct pAAV-CD151 and transiently transfect to HUVEC(human umbilical veine endothelical) mediated with Lipofectamine 2000.HUVEC were assigned to control group,GFP group,CD151 group.After transfection,the expression of CD151,Akt and phospho-Akt were measured by western blot.Proliferation of HUVEC were measured by MTT colorimetric assay.Results:①The expression of CD151,phospho-Akt of pAAV-CD151 transfected group were significantly increased compared with non-transfected group and pAAV-GFP transfected group(P

Objective To investigate the effects of CD151 on the expression of phosphoinositide 3-kinase(PI3K) and the proliferation of human umbilical vein endothelial cells(HUVEC) in vitro.Methods The plasmid p-AAV-CD151 was constructed and transfected into HUVEC.The proliferation activity of HUVEC was measured by MTT colorimetric assay.The expressions of CD151 and PI3K were tested by Western Blot.Results After transfection with p-AAV-CD151,the expressions of CD151 and PI3K were significantly increased compared with those of non-transfected group and p-AAV-GFP transfected group(P

Objective To investigate the protective effects of glycine on the lung injury in acute necrotizing pancreatitis(ANP). Methods Male Wistar rats were randomly divided into 4 groups: sham operation, ANP group,pretreated group and treatment group(with intravenous glycine 1g/kg). Serum TNF ? level,glutathione (GSH) and myeloperoxidase (MPO) in pulmonary homogenate were determined at 6, 12, and 24h hours after ANP induced. Pulmonary pathology and 3 day survival rate were compared between the groups.Results Compared with ANP group,in glycine pretreated group serum TNF ? level significantly reduced at 6,12, and 24h,while in glycine treatment group serum TNF ? level decreased at 24-hour . In glycine pretreated group or treatment group, the pulmonary GSH content were decreased.In both glycine pretreated group and glycine treatment group, pulmonary MPO activity decreased at 12 and 24h,and pulmonary pathologic severity significantly ameliorated and survival rate increased. Conclusions Although glycine can not increase the GSH synthesis,but giving prophylactic or therapeutic glycine may be effective in improving acute lung injury in ANP, probably through the inhibition of the infiltration and activation of leukocytes.

Objective To study the liver injury and effects of aescin on liver in rats with acute pancreatitis. Methods The rats were divided into 3 groups (control group, AP group and aescin group). The serum alanine aminotransferase (ALT), serum lactate dehydrogenase (LDH), hepatic cellular energy charge (EC) and adenosine triphosphate (ATP) were detected. The pathologic changes in pancreas and liver were also observed. Results The serum levels of ALT and LDH in aescin group were significantly lower than those of the AP group. The EC and ATP levels were significantly higher in aescin group than that of the AP group. Conclusion Introvenous injection of aescin can alleviate the liver injury in rats with acute pancreatitis.

Objective To investigate the protective effect of heme oxygenase-1 for xeno-heart transplantation and the possible mechanism.Methods Cobalt protoporphyrin (CoPP), HO-1 inducer, and zinc protoporphyrin (ZnPP), HO-1 inhibitor, were used to intervene donors and recipients on the model of NIH-Wistar cardiac transplants respectively and simultaneously some of recipients were treated with immunesuppressor cyclosporine A (CsA), and control group and CsA treated group were set up respectively. The expression of HO-1 protein, HO-1 mRNA, caspase-3 and STAT-3 in transplanted heart tissue was detected by immunohistochemistry, RT-PCR and Western Blot. At the same time, HO-1 enzymatic activity was examined in heart tissue, and the cardiac cell apoptosis was examined by means of TUNEL. The differences among various factors were compared.Results The survival time of cardiac transplants in CoPP group was longer than that in control and ZnPP groups (P

Objective To study the effect of interleukin 13(IL-13)on mucin gene Muc5ac and EGFR expression in mouse lungs. Methods Twenty-four male BALB/c mice were randomly divided into control group and different dose of IL-13 (50ng, 100ng and 250ng) groups, six mice per group. Muc5ac mRNA, and Muc5ac and EGFR protein expressions were detected by RT-PCR and immunohistochemical method, respectively. Results Both Muc5ac and EGFR positive products were located in the cytoplasm of airway epithelial cells. Compared with control group, IL-13 could increase Muc5ac mRNA, and Muc5ac and EGFR protein expressions in dose-dependent manner(all P

Ca~(2+)-activated chloride channels (CaCC, CLCA) are a new family of chloride channels discovered recently, which are related to Ca~(2+)-sensitive chloride ions transport, and there were several members identified currently in different biologic bodies. New studies have shown that its member gob-5 in murine (its human counterpart is CaCC1) plays a pivotal role in airway goblet cell metaplasia, mucus overproduction, MUC5AC gene expression as well as airway hyperresponsiveness (AHR) in asthma, and is a key molecule in the induction of murine asthma and an asthma-related gene. Inhibition of their function and/or their signaling pathway may therefore provide a new therapeutic strategy for asthma.

Objective To explore the mechanism of delayed xenograft rejection(DXR) by dynamic observation of histological and immunohistologic changes in mouse-to-rat cardiac xenografts.Methods A model of mouse-to-rat cardiac heterotopic xenotransplantation in neck was established by cuff technique.NIH mouse hearts not transplanted served as controls(n=4).Some xenograft recipients were killed and cardiac xenografts harvested at end of 3,8,16,24 h(n=4 for each time point) after transplantation.The cardiac hearts(n=16) of some xenograft recipients were not harvested until rejection time to determine their survival time.All heart samples were examined by HE and immunohistochemistry for semi-quantitative determination of antibodies including C3,IgM,IgG,E-selectin and macrophage marker——CD68.Results During the period after transplantation,the degree of rejection of xenografts became more and more serious till ultimate rejection.The mean survival time of the xenografts was(49.3?16.2) h.Immunohistochemical examination showed C3 were not detected in the xenografts at any time during the course of rejection;From 3 h after transplantation,obvious deposition of IgM was found in the grafts and IgG deposition got abundant;E-selectin expression was found as early as 3 h after transplantation and increased gradually;There was progressive infiltration by macrophages in the grafts.Conclusion Mouse-to-rat cardiac xenotransplantation can serve as an animal model of DXR.Endothelial cells activation,IgM and IgG,macrophage infiltration involve in DXR development except C3.

AIM: To study the effect of dexamethasone (DEX) on the adrenomedullin (ADM) and its gene expression in lung tissue of asthmatic rats. METHODS: 30 healthy male SD rats were randomly divided into three groups (10 for each). The asthmatic model was established by ovalbumin inhalation and injection. The mRNA expression of ADM was examined by RT-PCR and the protein expression was detected by immunohistochemical method. The airway wall thickness, the airway smooth muscle (ASM) thickness and pulmonary tissue changes were observed under light microscope. RESULTS: The expression of ADM mRNA and protein in the asthma group A were higher than those in the control group(group C) (P

Objective We aimed to study the status of nurse-physician collaboration in tertiary general hospitals and analyze the influencing factors,and provide references for improving the status and the service of medical-nursing quality.Methods Totally 280 nurses and 186 doctors working in medicine,surgery,emergency room,operating room and ICU in tertiary general hospitals were recruited in the study.The participants were investigated with the questionnaire of Nurse-Physician Collaboration Scale,Jefferson Scale of Attitudes towards Physician-Nurse Collaboration and the Tool for Organizational Culture.Results The status of nurse-physician collaboration was in a good condition,doctors scored (82.98±12.97),which was significantly higher than those of nurses (76.71±14.25),and there was sighificant difference.For doctors,the influencing factors included organizational culture,cooperative attitudes,gender,the type of contract; for nurses,the influencing factors included organizational culture,the department and marital status.Conclusions It is suggested to improve the condition of nurse-physician collaboration from the hospital managers and medical staff's point of view in tertiary general hospitals and construct the harmonious relationship between health care to provide high quality service for patients.