Sera from 80 patients suffering from gastritis and/or digestive ulcer were examined for IgG, IgA and IgM to Helicobacter pylori by enzyme-linked immunosorbent assay (ELISA). Anti-H. pylori IgG and IgA in serum could be used for diagnosis of H. pylori infection, but the former had good sensitivity (92.0%) and the latter had a great specificity (94.4%). Serum anti-H, pylori IgM level was not associated with H. pylori infection. There were no remarkable relationship between H. pylori infection degree arid levels of anti-H, pylori IgG IgA. The positive percentage of anti-H, pylori IgG of health population deceased after 50 years old but the anti-H, pylori IgA increased.

Objective To introduce the advance of the diagnosis and tretment of breast cancer(BC). Methods Reveiwing the recently world reference regarding to the diagnosis and treatment of BC was done for making an advancing paper of BC. Results The NF ?B and telomerase may be a new target of anti cancer of breast. The ECT, fiberoptic mammaroductoscopy, examine of micrometastase of BC, immuno cell chemical examine were progressing in the diagnosis of BC. The advance of the treatiment of BC included breast conservative surgery, lymphatic mapping and sentinel node biopsy, biochemical response modifier, new adjuvant chemotherapy, TAM and 1,25 (OH) 2D 3 treatmen. Conclusions The idea and mould of the diagnosis and treatment of BC have advanced obviously.

Objective To review the mechanisms of breast cancer escaping from host immune surveillance. Methods The current literatures on the mechanisms of breast cancer cells escaping from host immune surveillance were reviewed in the following aspects: alterations of MHC-Ⅰmolecule phenotype, deficiency of costimulatory molecules, apoptosis of T-lymphocytes induced by breast cancer cells presenting Fas ligand (FasL) and TNF-related apoptosis-inducing ligand (TRAIL) and host immune tolerance induced by tumor cells. Results Loss of classical antigen-presenting human leukocyte antigen (HLA) class-Ⅰmolecules, expression of non-classical HLA class-ⅠmoleculesHLA-G, loss of costimulatory molecule B7, apoptosis of T-lymphocytes induced by tumor cells presenting FasL and TRAIL and antigen-inducing host immune tolerance were related to breast cancer escaping from immune surveillance. Conclusion Breast cancer cells escape from host immune surveillance by altering MHC-Ⅰmolecules, lacking of costimulatory molecules, inducing of apoptosis of T cells and host immune tolerance. But the factors resulting in breast cancer escaping from host immune surveillance are not yet clear and should be further studied.

To observe the alterations of oxygen delivery/consumpation, the deformability and adherence of neutrophil in canine with respiratory distress syndrom(RDS), Method:The 15 selected healthy mongrel dogs were randomly allocated to receiving intravenous oleic acid 0.06 ml. kg~(-1) (test group, n= 8)or equivalent volume of saline (control group, n=7) respectively following anesthesia induction. The hemodynamics was monitored with Swan-Ganz catheter technique, the neutrophils were collected from arterial blood with centrifugal way to measure the deformability and adherence at 1st, 2nd, 4th and 6th hour after the administration of oleic acid separately. The pulmonary pathological examination underwent at 6th hour after the administration. Result: The pathological findings of test group were compatible with the RDS pathological characteristics. One hour after the administration, the deformability decreased significantly and the adheranee increased markedly (P

Objective To study the value of p27 and Ki-67 detection in differentiating benign from malignant thyroid follicular neoplasms. Methods Labelling index (LI) of thyroid neoplasms from thyroidectomy specimens were detected immunohistochemically Results The normal thyroid group had the highest expression of p27 , and the lowest expression of Ki-67. There was significant difference in the expressions of p27 and Ki-67 between follicular thyroid adenomas (FA) and follicular thyroid cancers (FTC) (P

Objective:To investigate the the effect of CD151 on Akt-Ser473 and human umbilical veine endothelial cell proliferation.Methods:To construct pAAV-CD151 and transiently transfect to HUVEC(human umbilical veine endothelical) mediated with Lipofectamine 2000.HUVEC were assigned to control group,GFP group,CD151 group.After transfection,the expression of CD151,Akt and phospho-Akt were measured by western blot.Proliferation of HUVEC were measured by MTT colorimetric assay.Results:①The expression of CD151,phospho-Akt of pAAV-CD151 transfected group were significantly increased compared with non-transfected group and pAAV-GFP transfected group(P

OBJECTIVE To investigate the risk factors of postoperative nosocomial infection in esophageal cancer patients,and to provide evidences for controlling nosocomial infection.METHODS The data of 466 cases with esophageal cancer,from Jan 2002 to Dec 2005,were studied retrospectively.RESULTS The nosocomial infection rate of patients with esophageal cancer was 21.03%.The main locations of infection were operation incision,lower respiratory tract and thorax.The nosocomial infection rate had increasing trend as lengthening the hospitalization duration.CONCLUSIONS The nosocomial infection with esophageal cancer is related to age,hospitalization duration and postoperative time.To take measures for infective factors,for example,intraoperative aseptic operation,postoperative drainage tube unblocked and rational use of antibiotics,is important to control and decrease the nosocomial infection.

Aim To study the effects of pyrrolidine dithiocarbamate(PDTC) on matrix metalloproteinase-9(MMP-9) expression in alveolar macrophages(AM) induced by TNF-?,and then to explore it's signal pathway.Methods AM were collected from bronchoalveolar lavage fluid in patients with chronic obstructive pulmonary disease.AM were pretreated with PDTC and then stimulated by TNF-? or IL-1.MMP-9 mRNA expression was detected by semi-quantitative reverse transcription-polymerase chain reaction and MMP-9 protein expression was detected by Western blot.Phospho-I?B? protein levels induced by TNF-? or IL-1 were detected by Western blot.NF-?B activity was detected by electrophoretic mobility shift assay.Results Both the mRNA and protein levels of MMP-9 induced by TNF-? in AM were significantly elevated in a dose dependent manner(P0.05).PDTC had distinct effect of inhibiting the activation of NF-?B induced by TNF-? or IL-1 in a dose-dependent manner(P0.05).Conclusions NF-?B plays an important role in MMP-9 expression induced by TNF-? in AM;PDTC can down-regulate MMP-9 expression induced by TNF? in AM possibly through preventing the degradation of I?B? via ubiquitylation-proteasome proteolytic pathway.

Objective To construct the expression vector maspin/pEFIRES-N so as to study maspin gene on the inhibition of the growth,invasion,and metastasis in hepatocellular carcinoma.Methods The full lenth of maspin gene was cloned and ligated to the expression vector pEFIRES-N digested by EcoRⅠ+XbaⅠ by T4 DNA ligase.The recombinant vector maspin/pEFIRES-N was stably transfected into hepatocellular carcinoma MHCC-97 cell line,and the expressive changes of maspin gene were detected.Results The recombinant plasmid was amplified in the E.coli.JM109.After the identification and sequencing,the reconstructive plasmid was confirmed containing the correct and full nucleotide sequence of maspin gene and the mRNA and protein level of maspin gene were up-regulated in hepatocellular carcinoma MHCC-97 cells.The proliferation and invasion of MHCC-97 cells was inhibited.Conclusion The expression vector maspin/pEFIRES-N was constructed successfully and could be expressed in eukaryotic cells.

Objective To investigate the diagnosis and treatment of primary retroperitoneal tumor,and improve the early correct diagnostic rate and the resection rate.Method 86 patients with primary retroperitoneal tumor(PRT) were analyzed by review of the clinical materials from 1990 to 2000.Results Abdominal or waist pain was the main symptom,and abdominal or waist mass was the main sign.52.6% percent of the patients got correct diagnosis before operation.62 cases were followed up,the 2-year survival rate for complete resection cases were 61.3%,while the patients who received palliative resection or biopsy died within one year.The resection rate of tumors was 81.3% in benign tumors and 37.7% in malignant tumors,the recurrent rates after complete resection were 15.4%,60%,respectively.Conclusions The earlier diagnosis,totally removal of tumors at first time,periodic examination after operation are three important ways.The operative treatment is very imperative for the patients with recurrence.