Objective To learn the effect of breast removal of polyacrylamide hydrogel by areola incision;and to provide an effective method for removal of polyacrylamide hydrogel.Methods According to the accepted operation,45 cases with breast polyacrylamide hydrogel were divided into 25 cases of observation group and 20 cases of control group.The observation group was treated with areola medial semicircular incision breast removal of polyac-rylamide hydrogel,and the control group was treated with areola small incision suction to remove polyacrylamide hydrogel.The operative time,intraoperative blood loss,postoperative drainage tube extraction time,postoperative 7d breast shape and perioperative complications of the two groups were observed;postoperative follow -up was performed to evaluate the effect of surgery.Results The operation time of the observation group (190.2 ±36.1)min was longer than (119.5 ±44.0)min of the control group,the difference was statistically significant between the two groups (t =5.924,P <0.05);the intraoperative blood loss of the observation group (250.4 ±23.9)mL was higher than (89.7 ± 30.4)mL of the control group,the difference was statistically significant (t =19.865,P <0.05 ).The difference between postoperative 7d breast normal morphology rates of the two groups was not statistically significant (χ2 =2.571,P >0.05);the postoperative drainage tube extraction time of the observation group (3.7 ±0.5)d was more than (2.3 ±0.3)d of the control group,the difference was statistically significant (t =11.021,P <0.05 ).The complication rate of the observation group was 12.0%(3 /25),which of the control group was 10.0%(2 /20),the difference was not statistically significant (χ2 =0.045,P >0.05).All cases of the two groups were followed up;the mean follow -up time was (6.7 ±2.1)months.The good rate of surgery of the observation group was 92.0 % (23 /25),which of the control group was 70.0%(14 /20),the observation group was higher than the control group (χ2 =5.718,P <0.05).Conclusion For patients with complications after polyacrylamide hydrogel injection for augmentation mammoplasty;the breast polyacrylamide hydrogel removal surgery by areola medial semicircular incision is recommen-ded as the preferred.

Objective To carry out a taxonomic identification of a strain of claviform bacteria iso-lated from prostatic fluid of a patient who suffered from chronic prostatitis, and to approach its phylogenic and biologic position. Methods We undertaked an initial identification by phenotypic characters such as morphologecal, physiological and biochemical characteristics to ascertain its phylogeny by chemical composi-tion analysis of cell wall and 16S rRNA gene sequencing and alignment. Results A club-shaped gram posi-tive rod bacillus was isolated in pure culture state. Its biochemical reactions were not active. The diamino-acid of cell wall was meso-diaminopimelic acid (meso-DAP) and it had wall chemotype Ⅳ ( contained arabi-nose, galactose and maltose ). Sequence searches of the GenBank database revealed that this strain had a highest level of 16S rDNA sequence similarity (99.4%) to C. tuberculostearicurn strain ATCC35692 with only 8 nucleotides difference. Conclusion On the basis of phenotypic and phylngenetie analysis, it is rea-sonable to assign this strain to the species C. tuberculostearicum, and this is the first isolation of C. tubercu-lostearicum from prostatic fluid home and abroad.

Based on quantum mechanics, rotational spectroscopy is a branch of fundamental science to study the rotational spectra of molecules, free radicals and ions, and has great applications on radio astronomy and many analytical fields such as atmospheric remote sensing. In this paper we reviewed the basic theory of rotational spectroscopy, two different types of Fourier transform microwave spectrometers, some typical spectroscopic analysis, and future prospect of the microwave spectroscopic techniques as well.

OBJECTIVETo study the efficacy of Viagra combined with behavior therapy against premature ejaculation (PE).

METHODSSixty PE patients were divided into two groups randomly: control group (behavior therapy alone) and the group of Viagra combined with behavior therapy. Intra-vaginal ejaculation latency time (IELT) and the coitus satisfaction of the patient and the partner were recorded before and after treatment.

RESULTSThe IELTs of the two groups were 0.80 +/- 0.20 and 0.73 +/- 0.24 minutes respectively before treatment, and 1.82 +/- 0.54 and 3.63 +/- 0.55 minutes respectively after treatment. As for IELT and satisfaction degree, Viagra produced better result than behavior therapy.

CONCLUSIONDuring this clinical trial, Viagra combined with behavior therapy prolonged IELT, which suggests that Viagra may be helpful for the treatment of premature ejaculation.

Adult ; Behavior Therapy ; Ejaculation ; drug effects ; Humans ; Male ; Middle Aged ; Piperazines ; therapeutic use ; Purines ; Sexual Dysfunctions, Psychological ; therapy ; Sildenafil Citrate ; Sulfones

Objective: To investigate the effect of astragalus polysaccharides (APS) on proliferation, invasion, apoptosis and drugresistance of cisplatin-resistant colorectal cancer (CRC) HT-29/DDP cells through regulating miR-20a/TGFBR2 axis, and to explore the possible mechanism. Methods: Human CRC HT-29 cells and HT-29/DDP cells were used as non-drug resistant and resistant cell models, respectively; HT-29/DDP cells were randomly divided into four groups, including untreated (HT-29/DDP) group, APS treatment group, miR-20a mimics + APS group, and si-TGFBR2 + APS group. qPCR and Western blotting were applied to detect the expressions of miR-20a and TGFBR2 in HT-29/DDP cells treated with different concentrations ofAPS (0, 0.5, 1.0, 1.5 and 2.0 mg/ml). Subsequently, dual luciferase reporter gene assay was used to verify whether TGFBR2 was a target gene of miR-20a. In addition, CCK-8, Transwell andAnnexin V-FITC/PI double staining were applied to examine the effect ofAPS on proliferation, invasion and apoptosis of HT29/DDP cells. Furthermore, subcutaneous HT-29/DDP cell xenograft model was established on nude mice, and the effect ofAPS on the growth of transplanted tumor was observed. Results: APS significantly inhibited the proliferation of HT-29/DDP cells in a dose-dependent manner (P<0.01). Meanwhile, the expression of miR-20a was down-regulated in HT-29/DDP cells treated with APS, while the expression of TGFBR2 was significantly up-regulated (all P<0.01). Additionally, dual luciferase reporter gene assay result showed that TGFBR2 was a direct target of miR-20a in HT-29/DDP cells and its expression was suppressed. Furthermore, APS could enhance the drug sensitivity of HT-29/DDP cells through downregulating the inhibitory effect of miR-20a on TGFBR2 expression, thereby suppressed proliferation and invasion, and induced apoptosis of HT-29/DDP cells in vitro and in vivo. It was also found that this effect was related with the suppression of PCNA and Bcl-2 proteins and promotion of Bax and Caspase-3 proteins. Conclusion: APS reverses the resistance of HT-29/DDPcells to cisplatin by down-regulating the inhibitory effect of miR-20a on TGFBR2 expression.