1.Effect of calpain inhibitor on hyperglycemia-induced apoptosis in cultured rat cardiomyocyte
Wenzhong WANG ; Yurong CHEN ; Zhenwang TANG
Journal of Chinese Physician 2012;(10):1354-1357
Objective To investigate the mechanism of inhibitor of calpain on hyperglycemia-induced apoptosis in cultured rat cardiomyocyte.Methods Cardiomyocytes were randomly divided into three groups (control,high glucose,and ALLN).MTT assay was used to detect the viability of cultured cardiomyocytes.Laser confocal microscopy was used to observe the mitochondrial permeable transition and membrane potential.The change of Caspase-3 activity in cardiomyocytes was detected by western blot.Results MTT assay showed that,after 72 h of hyperglycemia,the viability of cardiomyocytes was significantly declined (55% ± 11%),and the viability in the ALLN pretreatment group was (70% ± 15%) (P <0.05).After hyperglycemia,the mitochondrial permeable transition of cardiomyocyte was increased (30% ± 15% vs 60% ± 11%,P <0.05),and membrane potential was declined.Hyperglycemia could increase the expression of cleaved capsase-3,while with pretreatment of ALLN the expression of cleaved caspase-3 was downregulation(0.42 ± 0.11 vs 0.21 ±0.12,P <0.05).Conclusions The calpain inhibitor can protect cardiomyocytes from apoptosis under the high glucose condition.
2.NPC1L1 contributes to the inhibition of lipid accumulation induced by ezetimibe in RAW264.7 cells
Liang LI ; Haoyu YUAN ; Zhenwang TANG ; Weixia YU ; Zhizhong XIE ; Qinhui TUO ; Duanfang LIAO
Chinese Pharmacological Bulletin 2009;25(12):1563-1566
Aim To investigate the effect of intestinal cholesterol absorption inhibitor Ezetimibe on lipid accumulation in RAW264.7 cells and identify the underlying mechanism.Method RAW264.7 cells were pretreated with the indicated concentrations of Ezetimibe (0,0.003,0.01 and 0.03 mol·L~(-1))for 24 hours or pretreated with the optimal concentration(0.03 mol·L~(-1))of Ezetimibe for different periods (0,6,12 and 24 h),followed by incubation with 50 mg·L~(-1) oxLDL for 24 hours,then the number of intracellular lipid droplets and lipid content were measured by using oil red O staining and HPLC; the expression of NPC1L1 was measured by Western blot.Results Pretreatment with indicated concentrations of Ezetimibe caused a concentration-dependent inhibition of intracellular lipid accumulation;pretreatment with 0.03 mol·L~(-1) Ezetimibe caused a time-dependent inhibition of intracellular lipid accumulation.It was noted that pretreatment with 0.03 mol·L~(-1) Ezetimibe for 24 hours inhibited CE by about 47%+0.1% compared with control group(oxLDL alone).Immunoblotting results showed that NPC1L1 was expressed in RAW264.7 cells and it was down-regulated after Ezetimibe treatment.Conclusions Ezetimibe causes concentration-dependent and time-dependent inhibition of lipid accumulation in RAW264.7 cells;it also reduces NPC1L1 expression in RAW264.7 cells.
3. Prevalence and antimicrobial susceptibility of Salmonella isolated from broiler whole production process in four provinces of China
Weiwei LI ; Li BAI ; Xiuli ZHANG ; Xiaojie XU ; Zhen TANG ; Zhenwang BI ; Yunchang GUO
Chinese Journal of Preventive Medicine 2018;52(4):352-357
Objective:
To determine the prevalence and antimicrobial susceptibility of Salmonella isolated from broiler production process in 4 provinces of China.
Methods:
Using convenience sampling method, 238 sample sites from broiler whole production process were chosen in Henan, Jiangsu, Heilongjiang and Shandong provinces in 2012. A total of 11 592 samples were collected and detected to analyze prevalence baseline, including 2 090 samples from breeding chicken farms and hatcheries, 1 421 samples from broiler farms, 5 610 samples from slaughterhouses and 2 471 samples from distribution and retail stores. All