Objective: To experimentally research the supercritical carbon dioxide extraction of ? carotene in spirulina powder. Methods: Inspected the effect of such different condition as pressure (10～50MPa), temperature (20～50 ?C ) and flow rate of carbon dioxide (10～35kg/h) on the extract process. Results: The extraction yield was increased with increasing the pressure and temperature. Conclusion: The optimum operating condition of supercritical CO 2 extraction were as follows: pressure 25～35MPa and temperature 20～40 ?C and flow rate of CO 2 20～25kg/h.