1.PROTECTIVE EFFECT OF ASTRAGALUS MEMBRANCE ON MICE OF ACUTE TOXOPLASMOSIS
Yunqian LI ; Peijun HUANG ; Zhuming WANG ; Wen'E ZHAO ; Caiyun MA ; Zhenqing FENG ;
Chinese Journal of Schistosomiasis Control 1989;0(02):-
Objective To study the protective effect of Astragalus membrance on mice infected with tachyzoites of Toxoplasma gondii. Methods ICR mice were infected intraperitoneally with 10 5, 10 3, 10 2 tachyzoites of virulent RH strain of Toxoplasma gondii, and the mice were orally treated with Astragalus membrance 0 075 g/d per mouse or Azithromycin [150 mg/(d?kg)] each day starting from day 1 post-infection for 10 days. The survival rate and period were investigated. The parasite loads of livers and lungs of the mice infected with 10 2 tachyzoites were determined by fluorescence PCR methods at 4 day-post-infection (dpi) and 8 dpi. Results When infected with 10 5, 10 3 tachyzoites, treated with Astragalus membrance, the average survived days of the mice were 5 57 days and 6 23 days, and treated with azithromycin were 6 96 days and 8 12 days respectively. The azithromycin group but not the astragli group survived significantly longer than the control(P
2.Practice of Optimizing Experiment Teaching in Physiology
Zhuo FANG ; Tiejian ZHAO ; Xuemei LIU ; Xuelin DUAN ; Zhenqing HUANG ; Yuzhi QIN
Chinese Journal of Medical Education Research 2006;0(07):-
In view of the actual situation of our university,we carried out an experimental physiology teaching reform.By ways of preparing experiment guidance,setting conventional and potential experiment material,increasing choice of content and the opportunity for students to design experiments and optimizing experiment teaching processes in purpose of strengthening students' skills and promoting the training of application ability and innovation ability,we achieved high efficiency and low consumption of experimental physiology and improve the teaching quality.
3.CLONING AND SEQUENCE ANALYSIS OF THE LIGHT CHAIN VARIABLE REGION GENE OF MONOCLONAL ANTI-IDIOTYPIC ANTIBODY NP30 OF SCHISTOSOMA JAPONICUM
Xiaotong SONG ; Zhenqing FENG ; Zhenning QIU ; Yunqian LI ; Xiaocong YU ; Ying XIONG ; Changcheng YIN ; Hualiang HUANG ; Xiaohong GUAN
Chinese Journal of Parasitology and Parasitic Diseases 1997;0(05):-
Objective] To amplify and sequence the light chain of anti idiotypic monoclonal antibody NP30 of Schistosoma japonicum. [Methods] By comparing the conserved regions at each end of the nucleotide sequences of murine germ line genes enco ding FR1 and FR4 regions of immunoglobulin light chain variable regions, we designed a set of primers for amplification of V L gene. The hybridoma cells secreting anti idiotypic monoclonal antibody NP30 of Schistosoma japonicum were cultured and their genome DNAs were extracted and used as templates for PCR. The PCR product was then cloned into pUC19 vector. The recombinants were sequenced by Sanger′s method. The V L gene was compared with GenBank and published mouse V L genes. [Results] The full length of V L gene was 318 bp. The V L gene was a member of mouse Ig ? light chain subgroup IV and generated from rearrangement of germ line V and J? 4 genes. The V L gene sequence has been registered by GenBank(accession No. AF206720). [Conclusion] The obtained V L gene was a potentially functional gene of anti idiotypic monoclonal antibody NP30 of Schistosoma japonicum .
4.Quantitative MRI analysis of normal development of anterior and posterior acetabulum in children
Zhenqing LIU ; Hongsheng LIU ; Li HUANG ; Weiqiang XIAO ; Qianqian WU
Chinese Journal of Medical Imaging Technology 2018;34(4):601-604
Objective To investigate the normal development of anterior and posterior acetabulum in children through measuring anterior and posterior acetabular indexes with the baseline from the thinnest point of acetabulum to the center of femoral head.Methods MRI data in 165 normal children aged 0-12 years were collected.The baselines were drawn from the center of the femoral head to the thinnest point of acetabulum (method 1) and from the one midpoint of Y cartilage to the contralateral (method 2),then the anterior or posterior bony acetabular index (A/PBAI) and anterior or posterior cartilaginous acetabular indexes (A/PCAI) were measured.The consistency of above parameters measured using two methods and between two observers was observed,and the correlation with parameters-gestational ages was analyzed.Results The consistency of ABAI (ICC=0.832) measured with two methods was good,and the consistency of ACAI (ICC=0.535),PBAI (ICC=0.565) and PCAI (ICC=0.472) was fair.The consistency between two observers was good (all ICC>0.75).ABAI,ACAI and PBAI were negatively correlated with age (r=-0.762,-0.475,-0.368,all P<0.001),and PCAI had no correlation with age (r=-0.190,P<0.005).Before 4 years old,ABAI gradually decreased with age and gradually stabilized after 4 years of age.ACAI and PBAI decreased slightly with aging.PCAI did not change obviously with aging.Conclusion The measuring method of anterior and posterior acetabular indexes with the baseline from the thinnest point of acetabulum to the center of femoral head can accurately evaluate the normal development of anterior and posterior acetabulum in children.
5. Construction of latent membrane protein 2A chimeric antigen receptor-T cells and their lethal effects on nasopharyngeal carcinoma cells
Yuan CHEN ; Renjie CHEN ; Xiaochen HUANG ; Genxiong TANG ; Xingwang KUAI ; Mingjiong ZHANG ; Dawei ZHANG ; Qi TANG ; Jin ZHU ; Zhenqing FENG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2018;53(12):925-930
Objective:
To produce latent membrane protein 2A (LMP2A) chimeric antigen receptor (CAR)-T cells and detect the lethal effect of LMP2A CAR-T cells on nasopharyngeal carcinoma (NPC) cells.
Methods:
The study was conducted from September 2016 to December 2017.Genetic engineering technology was used to construct anti-LMP2A CAR lentiviral expression vector and sequencing was identified. The expression of anti-LMP2A CAR in the 293T cells was confirmed by western blot. CCK8 assay was used to evaluate the cytotoxicity of LMP2A CAR-T cells to NPC cells. ELISA assay was performed to test IL-2 and IFN-γ releasing of activated LMP2A CAR-T cells. The inhibition effect of LMP2A CAR-T cells on NPC xenograft tumor was observed