Objective To investigate the clinical effect of proximal femoral nail anti-rotation (PFNA) on femoral intertrochanteric fracture in the elderly.Methods Eighty-seven elderly patients from June 2007 to March 2012,who had femoral intertrochanter fracture and received treatment of PFNA,were enrolled in this study.Patients were comprised by forty seven people with stable fracture and forty people with unstable fracture.Results They were all followed up from 4.5months to 14 months,with the average time of 7.2 months.No death were found in hospital.The operation time was 25 min in average.During operation,losing blood was 80 ml.Ambulation time was 5.5 d,and the fracture healing time was 22 weeks in average.However,there were significant differences in intraoperative resetpostoperative position of the blade between the stable and unstable fracture patients (P＜0.01).Conclusions PFNA is one of the effective methods in the treatment of intertrochanter fracture,and it has shorter operation time,less losing blood,earlier ambulation,and hardly has complications.

Objective To explore the clinical effects of diabetic nephropathy treated with enalapril and Yinxingdamo injection. Methods 80 patients were randomly recruited into a control group and a treatment group. The control group was treated with enalapril, and the treatment group was treated with Yinxingdamo injection on the basis of the control group. Results Comparing with the control group, the treatment group could be seen a obvious decrease of 24h UAE, BUN and Cr(P<0.01). Conclusion The therapeutic effects of combined using enalapril and Yinxingdamo injection is better than only using enalapril in treating diabetic nephropathy.

Objective: To investigate the induction of Tca8113 cells to apoptosis by fadd gene.Methods: RT PCR and recombinant PCR were used to amplify human fadd gene and cloned into expression vector pcDNA3 and pIRES2 EGFP, then transfered into Tca8113 cells. The growth and apoptosis of the cells were tested by cell counting,fluorescent microscopy, electron microscopy and flow cytometery. Results: fadd gene was obtained and transfered into Tca8113 cells. After transfection of the gene the growth of the cells was inhibited by 25%～52%, cell number in G 1 phase increased and that in S phase decreased. Apoptosis of the cells was observed. Conclusion: fadd gene can effectively inhibite cell growth and induce Tca8113 cells to apoptosis.

Objective:To estimate the effect of HSV1-TK/GCV suicide gene therapy on human salivary gland epidermoid carcinoma cell line MEC-1. Methods: Expression vector G 1NAtK containing HSV-TK cDNA was transfected into MEC-1 cells.After transfection, the cells were selected by G418 for two weeks. The integrated gene and mRNA were detected with PCR and RT-PCR. The cytotoxicity and bystander effect were estimated by MTT and typan blue exclusion assay. The morphological changes after GCV treatment were observed with HE and 33258 stain and in situ cell apoptosis detection kit. Results: The 404 bp DNA fragment was amplified through PCR and RT-PCR in the transfected cells respectively. TK positive clone was named MEC-1/TK. The sensitivity of MEC-1/TK to GCV was 1 000 times more than that of parent MEC-1 cells.More than 90% of MEC-1 cells were killed by 10 ?g/ml of GCV when only 10% of MEC-/TK cells were present. The morphological changes included shrinking,detaching and floating of the cells. Some of the cells showed nucleus condensation and breakage of nucleus. A lot of cells showed nucleus positive in in situ apoptosis detection. Conclusion: HSV1-TK/GCV can confer MEC-1/TK cell killing efficiently. MEC-1/TK also has strong bystander effects. HSV1-TK/GCV system confers its effect, in part, by inducing apoptosis in TK positive cells.

Objective To explore the effect of propofol on tumor necrosis factor-alpha(TNF-?),interleukin-6(IL-6),interleukin-8(IL-8)in rats following renal ischemia/reperfusion and the protection of propofol on renal ischemia-reperfusion injury.Methods Seventy-two adult SD rats were randomly divided into three groups:sham operation group,ischemia/reperfusion group(I/R group)and propofol group(n=24 in each group).All the indexes of the last two groups were detected on 1,4 and 24 h after reperfusion.Five minutes before ischemia in propofol group,propofol was infused by vein and isometric physiologic saline to the other two groups.The concentration of IL-6,IL-8,TNF-? in serum and renal tissue,BUN and SCr in serum were measured.Hematoxylin and eosin staining for the renal tissue was processed.Results The concentration of IL-6,IL-8,TNF-? in serum and TNF-? in renal tissue in propofol group within 1 h after reperfusion was significantly lower than I/R group(P

Objective To study X-ray and clinical diagnosis of multiple myeloma(M M).Methods X-ray findings in 16 cases with M M were retrospectively analyzed.Results The main manifestations on X-ray were:normal in 3;osteoporosis in 8;osteolytic destrucion in 12;osteosclerosis in 1 and mass of soft tissue in 6.Conclusion The diagnosis of M M is mainly depended on clinical and X-ray findings,puncture of bone marrow is of diagnostic value,the differential diagnosis is still necessary.

Objeact:To evaluate the effects of the exogenous PTEN gene on morphology of the highly metastatic mucoepidermoid carcinoma cell line M3SP2. Methods: Morphological observation of vehicle transfected M3SP2-pBp cells and PTEN transfected M3SP2-PTEN cells was performed with inverted microscope, HE staining and optical microscope, scanning electronic microscope and transmisson electronic microscope. Results: Compared with the control cells of M3SP2-pBp, the exogenous PTEN expressing cells M3SP2-PTEN showed morphological changes, such as vacuole denaturalization, shrinkage, less microvillus, chondriosome swelling, lysosome amalgamation, and chromatin agglutination. Conclusion: The exogenous PTEN gene may induce denaturalization, necrosis, and apoptosis of the highly metastatic mucoepidermoid carcinoma cells.

objective:To study the growth inhibition effect of 5azaC on human salivary gland cell line HSG. Methods:HSG cells were exposed to 5-azaC at 5?10~ -6 mol/L and 10?10~ -6 mol/L respectively for 3 days. The proliferation of in vitro cultured HSG cells was studied by cell counting. The in vivo growth of HSG cells was investigated by tumor weight measurement in nude mouse models of HSG tumor induced by transplantation of the cells subcutaneously.Results:5 azaC inhibited HSG cell proliferation by 85% and 95% respectively at above mentioned doses. In the 3-week tumor growth study, the growth of the tumor induced by 5?10~ -6 mol/L 5azaC treated cell was inhibited by 74.8%.Conclusion:5azaC can inhibit the growth of HSG cells in vitro and in vivo.

Objective To study GAP\|43mRNA expression during nerve injure and regeneration. Methods Rat sciatic nerve was crushed then, in situ hybridization technique was used to explore GAP\|43mRNA expressions in spinal cord and dorsal root ganglion (DRG). Results The neurons of spinal cord and DRG were detected to have GAP\|43 hybridization sinal by 2 days after sciatic nerve lesion. At later times(4,7 and 14 days postsurgery) the anterior horn motor neurons and DRG cells showed an increase in the number of GAP\|43mRNA positive neurons, followed by a significant rise in their content of GAP\|43mRNA. The number of GAP\|43mRNA positive neurons was decreased by 30 days postinjure, and was nearly nomal 60 days postinjure.Conclusion GAP\|43mRNA expression was increased during peripheral nerve injure and regeneration. The study showed that GAP\|43 may play a key role in nerve regeneration. [