Objective To evaluate the feasibility and safety of the treatment for ventricular septal rupture (VSR) by transcatheter interventional therapy coordinated with percutaneous coronary intervention (PCI) or percutaneous transluminal coronary angioplasty (PTCA).Methods Six patients who suffered from cardiogenic shock due to acute myocardial infarction (AMI) and VSR were given intra-aortic balloon pump (IABP) and mechanical ventilation support. After three weeks, they underwent transcatheter closure of ventricular septal rupture with Amplatzer ventricular septal occluder, and at the same time PCI and PTCA were performed. Results All the patients successfully underwent ventricular septal defect occlusion, including two cases of residual shunt. Segmental stenosis of the middle of anterior descending branch and circumflex artery were noted in three patients, and then stent implantation was used. The other three cases had multi-vessel lesion but were without stent implantation. All the patients with stent implantation survived and discharged. Conclusions It is safe and feasible to apply transcatheter closure of ventricular septal rupture with Amplatzer ventricular septal occluder, coordinated with PCI and PTCA for patients with acute myocardial infarction complicated with ventricular septal rupture.

Objective To investigate a new method of measuring serum total protein by improving biuret double reagent through two point terminal method in order to dissolve the sample's interference of hemolysis, icterus, lipid , polymeric glucose.Methods The precision, recovery rate, the examination limit, the linear scope and the interference experiments were done by improving biuret reagent in order to ensure the method's basic function. The method was compared to Doumas method in order to ensure its reliability and the effection of dissolving the interference.Results The proportion of sample and reagent was 5∶220 ?l and the linear scope was 140 g/L. Regress equation:Y=484.0+48.06X,r=0.999 3.The within run CV(n=20) was 0.24% and the day to day CV(n=20) was 1.07%.The average recovery rate was 99.5%(95.6%~103%).The examination limit was 2.36 g/L. The interference was not significant when Hb concen tration was lower than 2.1 g/L, Bil lower than 148 ?mol/L,TG lower than 28.2mmol/L and polymeric glucose lower than 30 g/L. While measuring normal sample, improving method and Doumas method were highly correlation. Regress equation:Y=-2.435 4+1.037 4X,r=0.988 2. There wasn't a significant reference between results.Conclusion It could dissolve effectively the sample's interference of hemolysis, icterus, lipid, polymeric glucose and increase the result's accuracy while adopting improving biuret double reagent through two point terminal method.