Objective:To probe the method of isolating outer hair cells (OHC) from each of four turns of the guinea-pig cochlea. Method:From eight guinea pigs the organ of Corti from each of four turns of the cochlea were dissected, and then treated using enzyme. Result:A fair amount of living OHCs from each of four turns were obtained. The length of OHCs from each of four turns were 23.81,34.50,60.48 and 71.37 μm. Conclusion:The key to success in isolating OHCs from each of four turns of the cochlea is to know very well the anatomical characteristics of each of four turns of the cochlea and be operated in accordance with normal rules.