AIM: To investigate the effect of puerarin on the alteration of ET and ANF in plasma, NO and NOS in serum of diabetic rats induced by streptozotocin(STZ). METHODS: Rat modeling was set up by means of intraperitoneal injection of ST2 with a dose of 60mg?kg -1. Four days later, blood glucose was measured by glucose oxygen kit. On the basis of present documents, the rats that blood glucose was over 16.7mM were accepted as diabetic ones, and then were divided randomly into five groups: diabetic mellitus (DM), aminoguanidine (AG 0.1g?kg -1,ig.), puel (0.5g?kg -1,ig.),pue2(0.25g?kg -1,ig.), pue3(0.125g?kg -1,ig.) and a normal group additionally. In order to set up a chronic model, we observed 12 weeks according to our past experiments. The FBS was measured at the twelfth week using the test kit. NO and NOS in serum were determined by checking their OD values, ET and ANF in plasma were determined by radio-immunization, respectively. RESULTS: In diabetic rats, Plasma ET and ANF levels increased obviously, NO and NOS levels decreased greatly (P

AIM To investigate the effect of puerarin on the formation of advanced glycation end products (AGEs) and RAGE(receptor for advanced glycation end products)mRNA expression in the arota of diabetic rats induced by streptozotocin (STZ). METHODS Rats were injected streptozotocin into intraperitonel with a dose of 60 mg?kg -1 . Four days later, blood glucose was measured using glucose oxygen kit. The rats with over 16 7 mmol?L -1 blood glucose were considered as diabetic ones, and then divided randomly into five groups: diabetic mellitus(DM), aminoguanidine(AG 0 1 g?kg -1 , ig), pue1(0 5 g?kg -1 , ig), pue2(0 25 g?kg -1 , ig) and pue3(0 125 g?kg -1 , ig). In order to set up a chronic model, we observed 12 weeks according to our past experiments. The fasting blood glucose(FBG) was and the contents of serum fructosamine (FMN) were measured using the test kit. The quantity of AGEs accumulation, the expression of RAGE in the arota was detected using fluorescence method and reverse transcription polymerase chain reaction(RT PCR). RESULTS The contents of FBG and FMN, the accumulation of AGEs in the aorta of rats treated with puerarin were lower than that of diabetic model rats( P

Objective: Qualitative and quantitative determination of the contents of inorganic elements in Corium Bufo bufo gargarizans Cantor. Methods: Qualitative determination by atomic emission spectrometry and quantitative determination by ICP AES.Conclusion: The content of calcium is the highest in Corium Bufo bufo gargarizans Cantor.

s:Cardiomyocyte apoptosis and regeneration are present in overload cardiac hypertrophy. Cardiac myocyte apoptosis might be a critical factor during the transition from compensatory hypertrophy to heart failure. The mechanisms of apoptosis include extrinsic factors and some cellular signal pathways. On the other hand, cardiomyocyte hyperplasia, as well as hypertrophy, contributes to cardiac hypertrophy. Caridomyocyte regeneration is related to cyclin, cyclin dependent kinase (Cdk), Cdk inhibitors (CdkI), bcl-2, p53 and telomere. Cardiaomyocyte regeneration and apoptosis are linked through several factors.

Invasion and metastasis are the distinct biological characterstics of cancer. They are the key factors influencing tumour patients' theraputic effect and prognosis. Recent study shows that metalloproteinases can facilitate tumour cell invasion and metastasis. In this review we evaluate the novel aspects of the function of matrix metalloproteinases in tumor invasion and metastasis. The regulation of the expression and activity of MMPs are summarized. The potential clinical application of metalloproteinase and their tissue inhibitors were also presented.

Objective To study the antioxidation and immuno-regulation effects of the active fraction in Jiawei Zhisou Powder.Methods Hypersensitive asthma mice were established by the miscible liquids of ovalbumin(OA)and Al(OH)3.The influences of the powder on the contents of SOD and MDA in the serum and lung tissue of mice were observed and the contents of IL-4 and IFN-?in supernatant of mouse spleen cells cultured in vitro were measured by enzyme-linked immunosorbent assay.And Jiawei Zhisou Powder and dexamethasone were administered to normal mice respectively,then the immune organs weight were measured and the spleen and thymus indexes of mice were calculated.Results Compared with the normal group,the contents of SOD and IFN-?were significantly decreased,while MDA and IL-4 contents were obviously enhanced in asthma group(P