1.Enforcement of Programmed Management in the Introduction of New Drugs
China Pharmacy 2005;0(22):-
OBJECTIVE:To set up and improve the procedures and methods for the introduction of new drugs into hospital.METHODS:A supervising system on the introduction of new drugs was established and a programmed management on the introduction of new drugs was put into practice.RESULTS & CONCLUSIONS:A series of measures such as establishing expert database for the evaluation of the introduction of new drugs,assuming supervision throughout the course carried out by discipline inspection committee,increasing clinical pharmacy evaluation and monitoring after the introduction of new drug,etc.have achieved good outcome in the management on the introduction of new drugs,which help promote hospital's fair,transparent and scientific introduction of new drugs.
2.Study on the extracting method of salviae miltiorrhizae madix et rhizoma in Huoluo-Xiaoling granules
Chao WANG ; Yuanyan LIU ; Qing LI ; Lei XIA ; Zhiqian SONG ; Chun WANG ; Yuna CAO ; Zheng WEI ; Linyan ZENG ; Zhenli LIU
International Journal of Traditional Chinese Medicine 2011;33(7):605-608
Objective To optimize the extraction method of salviae miltiorrhizae madix et rhizoma in Huoluo-Xiaoling granules. Methods According to the contents of Salvianolic acid B and Tanshinone Ⅱ A, the extraction method was established by comparing different solvents (water and 70% ethanol)and extracting modes (compound extraction and single herb extraction). Then orthogonal design was used to determine the optimum extraction method. Results Considering the contents of salvianolic acid B and tanshinone Ⅱ A, 70% ethanol extract was better than water extract and compound extraction was better than single herb extraction.The optimum extraction condition was 70% ethanol in eight times of the herbs weight, extracted for 1h by 3times. Conclusion The extraction method was simple and stable.
3.Analysis of MVK gene variant in a child with high IgD syndrome caused by mevalonate kinase deficiency.
Junchao WANG ; Xingjia WEI ; Zhenli TAO
Chinese Journal of Medical Genetics 2022;39(4):413-416
OBJECTIVE:
To analyze the clinical and genetic features of a patient with mevalonate kinase deficiency (MKD).
METHODS:
Whole exome sequencing was carried out for the proband. Candidate variant was verified by Sanger sequencing.
RESULTS:
The proband was found to harbor compound heterozygous variants of the MVK gene, including a c.248C>T (p.Phe83Cys) variant derived from his father and a c.971C>T (p.Ala324Val) variant from his mother. Based on the guidelines of the American College of Medical Genetics and Genomics, both variations were predicted to be likely pathogenic (PM1 + PM2 + PM3 + PP3).
CONCLUSION
The compound heterozygous variants of the MVK gene probably underlay the MKD in the proband. Above findings have enriched the mutational spectrum of the MVK gene.
Child
;
Genomics
;
Humans
;
Immunoglobulin D/genetics*
;
Mevalonate Kinase Deficiency/genetics*
;
Mutation
;
Whole Exome Sequencing
4.Determination of alpha-pinene and octyl acetate contents in Boswellia serrata.
Zhiqian SONG ; Lei XIA ; Zheng WEI ; Yuna CAO ; Linlin ZHANG ; Zhenli LIU
China Journal of Chinese Materia Medica 2012;37(10):1431-1433
OBJECTIVETo establish method for determining the contents of alpha-pinene and octyl acetate in Boswellia serrata, in order to provide preference for making quality standards for B. serrata and processed B. serrata.
METHODApplication of orthogonal design was employed to optimize the solvent, solvent quantity and extraction time. The GC-MS analysis was performed on a Rxi-5ms silica capillary column, running in the electron impact (EI) mode, with ion trap and injector temperature of 200 degrees C and 250 degrees C, respectively. The column oven was initially 50 degrees C and was held for 1 min after injection, followed by temperature ramping at 5 degrees C x min(-1) up to 130 degrees C, holding for 1 min. 1 microL of samples solution were injected in the split mode (1:60). Helium was the carrier gas. The mass spectrometer was set to scan m/z 45450 with an ionizing voltage at 70 eV.
RESULTSample solutions were prepared for 50-fold dose by ultrasonic extraction with hexane for 30 min. The content of alpha-pinene and octyl acetate in 10 batches of B. serrata were 0.021 3-0.149 5, 2.519 6-9.098 0 mg x g(-1), respectively. And, those of alpha-pinene and octyl acetate in processed B. serrata were 0.015 9-0.065 9, 0.801 0-12.812 2 mg x g(-1).
CONCLUSIONThe method is a stable and reliable for determining the contents of alpha-pinene and octyl acetate in B. serrata.
Acetates ; analysis ; Boswellia ; chemistry ; Gas Chromatography-Mass Spectrometry ; methods ; Monoterpenes ; analysis