BACKGROUND: The Chinese medicine, Sheng-Ji Ointment, is utilized to cure bone defect due to infectious open fractures in the clinical field. So it was imagined that it was a new inductive factor of osteogenesis and its ingredients could acceleate the proliferation of bone marrow-derived mesenchymal stem cells (BMSCs) and induce them into osteoblasts. In our primary experiment, the Sheng-Ji liquor, which was extracted from the same Chinese herbs with Sheng-Ji Ointment, had been identified that it affected the proliferation of BMSCs with its different concentration.OBJECTTVE: To investigate the effect of Sheng-Ji liquor on the osteogenesis of in vitro cultured BMSCs from rabbit tibia.DESTGN: An observation in single kind of sample.SETTING: Orthopaedic Research Institute of Tianjin Hospital.MATERIALS: The experiment was carried out in the Tissue Engineering Laboratory of Tianjin Orthopadic Institute of Integrated Chinese and Western Medicine from October 2004 to December 2005. BMSCs were obtainedfrom male healthy Japanese long-eared rabbits of 3 months old, (3.5±0.5) kg. Sheng-Ji liquor (5×102 g/L) was extracted from the Chinese herbs with Sheng-Ji Ointment (Tianjin Factory of Chinese Crude-drug and Cut Crude-drug), including the crude drugs of angelica, rehmannia dride rhizome, carapax testudinis, corium elephatis and crinis carbonisatus.METHODS: The Sheng-Ji liquor was put to BMSCs in different concentrations of 25, 8.3, 5 g/L. Basic medium without Sheng-Ji liquor was taken as blank control, while the standard-controlled medium contained the ingredients of the following blank-controlled medium, dexamethasone 10-8 mol/L, vitamin C 0.05 g/L and β-sodium glycerophosphate 10 mmol/L. The BMSCs passaged in different conditions were seeded in plate and cultured for 2-3 weeks, then the mineralized nodes were observed under inverted fluorescence microscope, and the cell expressions were observed with acheomycin labeling and collegan Ⅰ immunohistochemical staining. The culture medium was collected to determine the contents of extracellular and intracellular alkaline phosphatase (ALP), osteocalcin (OCN) and lactate dehydrogenase (LDH), then the ratios of ALP/LDH and OCN/LDH were calculated.MAIN OUTCOME MEASURES: After 2 and 3-week culture, the mineralized nodes, results of acheomycin labeling and collegan Ⅰ immunohistochemical staining, and the ratios of ALP/LDH and OCN/LDH were observed.were prolonged, proliferated assembly and overlapped. The inter-cell limits disappeared gradually and sporadic cellular Results of acheomycin labeling and collegan Ⅰ immunohistochemical staining: There were positive cells and mineralized nodes in all of groups with Sheng-Ji liquor and standard-controlled group. BMSCs of the blank-controlled group were negative. The positive cells and mineralized nodes were green with yellowish color under irrigation of fluorescence; The results of immunocytochemistry for collagen Ⅰ were positive in all of groups with Sheng-Ji liquor and standard-controlled group. The result of blank-controlled group was negative. The positive induced BMSCs had the cytoplasm with granula of highest as compared with the other groups (P ＜ 0.05). The ratios of ALP/LDH and OCN/LDH in the other groups were close (P ＞ 0.05).

Insulin sensitivity,β-cell function and serum high-sensitivity C-reactive protein (hs-CRP)levels were observed in obese and non-obese normoglycemic first-degree relatives of type 2 diabetic patients (FDR). The results showed that there existed insulin resistance,β-cell dysfunction and increased serum hs-CRP level in obese FDR of type 2 diabetic patients. Moreover, insulin resistance and increased CRP level were positively related to waist circumference.

Objective:To study the application of three-dimensional(3D) printed individualized tray in impression taken of presurgical nasoalveolar molding(PNAM) for newborn with cleft and palate.Methods:The newborns with unilateral/bilateral cleft lip and palate treated with PNAM during 2015 to 2020 in School and Hospital of Stomatology, China Medical University, were chosen. Their clinical materials were retrospectively analyzed. Maxillary impressions taken by 3D printed individualized trays were compared with those taken by modified traditional tray. The indexes were evaluated including the numbers of defects (bubbles with diameter>1 mm and exposure of tray) in each impression; the impression quality grade (perfect 1, good 2, qualified 3 and unqualified 4), and the integrity and legibility of impressions [evaluated by 3 orthodontists using visual analog scale(VAS)]. The date was analyzed by SPSS 23.0. Rank-sum test was used for comparison between groups.Results:A total of 22 patients were included. There were 7 patients (3 males, 4 females, 3-28 days old) in modified traditional tray group and 15 patients (10 males, 5 females, 4-26 days old) in 3D printed individualized trays group. All patients finished the PNAM treatment before repairment of cleft lip. The average treatment duration were 136 days with 131 days in modified traditional tray group and 137 days in 3D printed individualized trays group. (1) The impressions made by conventional method had more defects [6(5, 7)] than those made by 3D printed individualized trays[2(1, 2)]( P<0.05). (2)The impression taken by modified traditional tray was mainly qualified[3(2, 3)scores] and that taken by 3D printed individualized tray exhibited higher grade [good, 2(2, 2) scores]( P<0.01). (3) Compared with modified traditional tray group, the scores of VAS on integrity of impression taken by 3D printed individualized tray were significantly high( P<0.05). Itemized evaluation showed that the integrity scores on all positions except vestibular sulcus had significant difference between groups( P<0.05 or P<0.01). The scores of VAS on legibility of impression taken by 3D printed individualized tray were significantly higher than those taken by modified traditional tray( P<0.05). As for itemized evaluation, legibility scores on all positions except upper frenum had significant difference between groups( P<0.05 or P<0.01). Conclusions:The impression taken by 3D printing individualized tray has higher quality, integrity and legibility.

Objective:To study the application of three-dimensional(3D) printed individualized tray in impression taken of presurgical nasoalveolar molding(PNAM) for newborn with cleft and palate.Methods:The newborns with unilateral/bilateral cleft lip and palate treated with PNAM during 2015 to 2020 in School and Hospital of Stomatology, China Medical University, were chosen. Their clinical materials were retrospectively analyzed. Maxillary impressions taken by 3D printed individualized trays were compared with those taken by modified traditional tray. The indexes were evaluated including the numbers of defects (bubbles with diameter>1 mm and exposure of tray) in each impression; the impression quality grade (perfect 1, good 2, qualified 3 and unqualified 4), and the integrity and legibility of impressions [evaluated by 3 orthodontists using visual analog scale(VAS)]. The date was analyzed by SPSS 23.0. Rank-sum test was used for comparison between groups.Results:A total of 22 patients were included. There were 7 patients (3 males, 4 females, 3-28 days old) in modified traditional tray group and 15 patients (10 males, 5 females, 4-26 days old) in 3D printed individualized trays group. All patients finished the PNAM treatment before repairment of cleft lip. The average treatment duration were 136 days with 131 days in modified traditional tray group and 137 days in 3D printed individualized trays group. (1) The impressions made by conventional method had more defects [6(5, 7)] than those made by 3D printed individualized trays[2(1, 2)]( P<0.05). (2)The impression taken by modified traditional tray was mainly qualified[3(2, 3)scores] and that taken by 3D printed individualized tray exhibited higher grade [good, 2(2, 2) scores]( P<0.01). (3) Compared with modified traditional tray group, the scores of VAS on integrity of impression taken by 3D printed individualized tray were significantly high( P<0.05). Itemized evaluation showed that the integrity scores on all positions except vestibular sulcus had significant difference between groups( P<0.05 or P<0.01). The scores of VAS on legibility of impression taken by 3D printed individualized tray were significantly higher than those taken by modified traditional tray( P<0.05). As for itemized evaluation, legibility scores on all positions except upper frenum had significant difference between groups( P<0.05 or P<0.01). Conclusions:The impression taken by 3D printing individualized tray has higher quality, integrity and legibility.

The biological samples of oral genetic diseases and rare diseases are extremely precious. Collecting and preserving these biological samples are helpful to elucidate the mechanisms and improve the level of diagnose and treatment of oral genetic diseases and rare diseases. The standardized construction of biobanks for oral genetic diseases and rare diseases is important for achieving these goals. At present, there is very little information on the construction of these biobanks, and the standards or suggestions for the classification and coding of biological samples from oral and maxillofacial sources, and this is not conducive to the standardization and information construction of biobanks for special oral diseases. This consensus summarizes the background, necessity, principles, and key points of constructing the biobank for oral genetic diseases and rare diseases. On the base of the group standard "Classification and Coding for Human Biomaterial" (GB/T 39768-2021) issued by the National Technical Committee for Standardization of Biological Samples, we suggest 76 new coding numbers for different of biological samples from oral and maxillofacial sources. We hope the consensus may promote the standardization, and smartization on the biobank construction as well as the overall research level of oral genetic diseases and rare diseases in China.