Objective To explore the relationship between calcium-sensing receptor's expression and papillary thyroid carcinoma.Methods Seventy cases of postoperative papillary thyroid carcinoma were selected.Immunohistochemical technique was used to detect expression of calcium-sensing receptor in papillary thyroid carcinoma,thyroid benign lesions,and normal thyroid tissue,respectively.SPSS 17.0 statistical analysis was used with non parametric test,P＜0.05 indicated significant difference.Results The expression of calcium-sensing receptor in papillary thyroid carcinoma,benign thyroid,and normal thyroid were significantly different (90％,40％,0％,respectively; P ＜ 0.05).The expression of the calcium-sensing receptor in the group of papillary thyroid carcinoma with calcification was significantly higher than that in thyroid cancer without calcification (P ＜ 0.05).Conclusions This study suggests that the calcium-sensing receptor may be associated with papillary thyroid carcinoma and may play a key role in the calcification of the carcinoma.

[Objective]To explore the surgical plan of lumbar degenerative de novo scoliosis combined with vertebral stenosis(LDDS) and evaluate the surgical efficiency.[Method]From July 2003 to Jan.2007,32 LDDS patients were diagnosed and operated in the author’s hospital.The X-ray,computed tomography and MRI radiological data were measured carefully preoperately which included the Cobb’s angle,vertebral rotational and lateral subluxation degree.All the patients were treated by posterior decompression and pedicle screw internal fixation,and the surgery,fluoroscopic time and bleeding amount were recorded.Patients were administered pre- and postoperatively with clinical outcome surveys of Visual Analogue Pain Score(VAS),Low Back Outcome Score(LBOS).Pre- and postoperative measurements of the Cobb's angle and lumbar lordosis of the involved segments were analyzed.[Result]The surgery time was 4?0.5 hours,the average bleeding amount was 400?30 ml,the fluoroscopic time was 30?5 min.All the patients were followed from 8 months to 4 years,and radiography was proceeded on 8 and 16 months respectively post surgery.Twenty-four cases got good bone fusion,and mean Cobb's angle decreased from 37.2 to 18.4 degrees,mean focal lordosis increased from 13.1 to 16.4 degrees,mean vertebral rotation angle decreased from 14.2 to 9.8 degrees,mean preoperative measures of VAS and LBOS improved from 5.3?2.2 and 24.8?15.6,respectively to 2.2?2.1 and 44.8?18.0 at last follow-up,the Cobb’s angle,lumbar lordosis and the degree of vertebral rotation had significantly difference pre and post surgery(t test,P

[Objective]To study the clinical characteristics of osteochondrosis of lumbar posterior vertebral edge(OLPV) and evaluate the efficiency of surgical treatment. [Method]From August 2000 to August 2003,41 cases of OLPV patients were treated,all cases underwent decompression of posterior approach and selective resection of the compressive things.Furthermore,1 case was treated with trans-vertebral pedicle internal fixation and inter-transverse process bone graft because of excessive decompression and preoperative lumbar instability.[Result]Low back and leg pain disappeared or obviously being relieved in all cases,and the results of histological analysis suggested the diagnosis of OLPV in all cases.Forty-one cases were followed from 5 to 8 years,average 6.8 years.No lumbar instability or spondylolisthesis was found in any case.Six cases suffered slight low back pain occasionally without obvious impaired quality of life.The preoperative JOA score was 14.73?2.06,when followed by 3 months,the score changed into 27.14?4.13,there was statistical significance between them(P

[Objective]To analyze the dynamic changing of inflammatory markers in second stage revision for infected TKA and to explore the sensitive indicators. [Methods]From June 2004 to April 2008,20 cases of infected TKA were admitted and treated by the two stages knee revision.The inflammatory markers included full blood count,erythrocyte sedimentation rate(ESR).C-reactive protein(CRP) were examined weekly,and to evaluate the dynamic changes and the most sensitive indicators.[Results]CRP was the most sensitive index for infection control,and its maximum presented in the first week post antibiotic bone cement implantation,followed by rapid reduction in the second week and there was statistical difference compared to the CRP level before surgery(t=105.84,P

Objective To explore the relationship between intestinal fatty acid binding protein(FABP2) gene G54A polymorphism and obesity,the effect of mutant 54A FABP2 gene on serum lipids metabolism.Methods The total of 84 subjects with obesity and 60 subjects with normal weight were involved in this study.The G54A FABP2 gene allele and genotype frequencies were detected by polymerase chain reaction (PCR)-restriction fragment length polymorphism(RFLP) technology.The automatic biochemical Analyzer was used to detect triglyceride(TG),high-density lipoprotein cholesterol(HDL-C) and low-density lipoprotein cholesterol(LDL-C) levels.Results The results of study on FABP2 gene polymorphism revealed as followed:in obese groups,the frequencies of GG,GA,A/A genotypes was 19.0%(16/84),73.8%(62/84) and 7.2%(6/84),respectively;in control group,the frequencies of G/G,G/A,A/A genotypes was 38.3%(23/60),58.3%(35/60),3.3%(2/60),respectively;the differences between two groups was statistically significant(χ2=6.97,P<0.05).In obesity group,the frequencies of alleles were 56.0%(94/168) for 54G and 44.0%(74/168) for 54A;in the control group,the frequencies of alleles were 67.5%(81/120) for 54G and 32.5%(39/120) for 54A;the differences between two groups was statistically significant(χ2=3.92,P<0.05).The plasma biochemical variables results showed as followed:in obesity group,the carriers of A/A homozygous genotypes and G/A heterozyous genotypes both had significantly higher plasma TG((2.36±0.82) mmol/L,(2.06±0.59) mmol/L,respectively)and LDL-C((3.94±0.96) mmol/L,(3.29±0.55) mmol/L,respectively)level than those with GG wild genotype(t=2.206,2.575,2.632,2.278;P<0.05).The level of HDL-C in the carriers of A/A homozygous genotypes((1.23±0.34) mmol/L)and in the carriers of G/A heterozyous genotypes((1.21±0.26) mmol/L) were not significantly different than those with GG wild genotype((1.29±0.31) mmol/L,P>0.05).The carriers of A/A homozygous genotypes had significantly higher plasma LDL-C((3.94±0.96) mmol/L)level than thosewith G/A wild genotype((3.94±0.96) mmol/L vs.(3.29±0.55) mmol/L,t=2.476,P<0.05),but the plasma TG((2.36±0.82) mmol/L vs.(2.06±0.59) mmol/L;P>0.05) and HDL-C((1.23±0.34) mmol/L vs.(1.21±0.26) mmol/L;P>0.05) level had not difference.Conclusion The FABP2 gene G54A polymorphism is related to obesity and lipid metabolism abnormality.The allele encoding in FABP2 gene may be a potential factor contributing to promoting lipid metabolism abnormality.

Objective To study general law of typing according to syndrome differentiation of Chronic Obstructive Pulmonary Disease (COPD). Method Based on large sample prospective Clinical Epidemiology investigation, symptoms, conditions of tongue, pulse according to TCM and pulmonary function, quality of life in 616 patients of COPD were collected. By means of Frequencies procedure, factor analysis, cluster analysis and analysis of variance in SPSS11.5, analyze the investigated data and lay difficient types of syndrome of COPD according to TCM. Meanwhile, explored distinct distribution and the relation between types of syndrome and pulmonary function, quality of life in patient5 with COPD. Result There were six types according to syndrome differentiation of COPD, deficiency of lung-qi, pathogenic wind-cold attacking lung, deficiency of pulmonosplenic both vital energy and yin, yin-deficiency of the lung and kidney, stagnation of phlegm and pathogenic fluid-reteneion in lung following splenic asthenia and stagnation of liver-qi, blood stasis-phlegm following deficiency of heart-yang and kidney-yang. There were multiple types of syndrome in COPD patients, the relation was detected between each type of syndrome and lung function, quality of life. Conclusion To draw assistance from clinical epidemiology and mathematical statistics method, the initial standard of differentiation of symptoms and signs to COPD was layed. The regularity of types of syndrome to COPD was detected. The result could be used in diagnosis of COPD, and the method could be communicated in the standardization research of syndrome differentiation.

Objective:The immunodeficiency animals play more and more important role in oncology research.But researchers usually do not how to choose kinds of immunodeficiency rats and mice during experiments.In this paper, applications and characteristics of immunodeficiency animals were compared as well as some of the common problems were discussed in oncology study.Methods:Classification of the immunodeficiency animals, the characteristics and application of congenital immunodeficiency rats and mice, and common problem analysis in the study of oncology were explored.Results: The feature of various immunodeficiency rats and mice should be considered in the oncology experiments.Conclusion:The paper provide valuable reference for scientific researcher.

Objective To observe effect of aerobic exercise combined with inhalation of tiotropium on patients with stable chronic obstructive pulmonary disease (COPD) complicated with pulmonary hypertension.Methods A total of 98 patients with stable COPD and grade Ⅱ or Ⅲ pulmonary function complicated with pulmonary hypertension were recruited.They were divided into an observation group (n =31) and two control groups (n =30 and 37).Patients in control group 1 inhaled shahmette and fluticasone powder,and antibiotics as well as apophlegmatisant were also used in accordance with the demands of the patient's condition.Patients of in control group 2 inhaled tiotropium dry powder and accepted other therapeutic measures equivalent to those of control group1.The patients in the observation group were given aerobic exercise in addition to other therapeutic measures equivalent to those of control group 2.Six-minute walk test distances,pulmonary arterial systolic pressure and chronic obstructive pulmonary disease assessment test scores were compared after 6 months of treatment,among the groups and against the baseline values before treatment.Results After 6 months of treatment the average 6-minute walk distance,chronic obstructive pulmonary disease assessment score and pulmonary arterial systolic pressure of the observation group were all significantly better than for either of the control groups.Conclusions Inhaling tiotropium dry powder combined with aerobic exercise can reduce pulmonary artery systolic pressure and improve the symptoms as well as the quality of life for patients with stable COPD complicated by pulmonary hypertension.

Objective To study the different expression of microRNA(miRNA) including mir-29c,mir-200a,mir-145 in the mid-secretary endometrium and its relationship with infertility of endometriosis.Methods From August 2011 to February 2013,36 infertile cases with endometriosis confirmed by laparoscopy and pathology and excluded the other infertile factors in Department of Reproductive Medicine in Peking University Shenzhen Hospital were enrolled in this study,which were divided into 17 cases with stage Ⅰ-Ⅱ,19 cases with stage Ⅲ]-Ⅳ according to the revised classification American Fertility Society.Forty-four healthy women with male factor infertility were chosen as control group.The relative expression levels of mir-29c,mir-200a,mir-145 in the endometrium of women in two groups were detected by using real-time quantitative polymerase chain reaction.Those women were followed up for pregnancy outcome of endometriosis group after assisted reproductive techniques (pregnancy and nonpregnancy group respectively).Results (1) The expression of miRNA between endometriosis and control groups:the average expression level of mir-29c,mir-200a,mir-145 in the endometrium of endometriosis group was 2.46 ± 1.98,3.20 ± 2.45,6.378 ± 3.275,which were significantly higher than 1.36 ± 1.05,2.04 ±1.16,4.548 ± 1.885 in control group (P =0.026,0.027,0.041,respectively).(2) The expression of miRNA between Ⅰ-Ⅱ stage and Ⅲ-Ⅳ:the average expression level of mir-29c,mir-200a,mir-145 in the endometrium was 0.53 ± 0.51,0.33 ± 0.26,0.048 ± 0.021 in patients with of stage Ⅰ-Ⅱ,while 0.26 ±0.18,0.28 ±0.12,0.045 ±0.016 in stage Ⅲ-Ⅳ respectively,there were no statistically significant differences between the two groups(P =0.191,0.661,0.753,respectively).(3) The expression of miRNA between pregnancy and non-pregnancy groups:the average expression level of mir-29c,mir-200a,mir-145 in the endometrium were 0.60 ± 0.30,1.23 ± 0.48,0.886 ± 0.238 in pregnancy group,while 2.64 ± 1.73,4.39 ± 2.58,7.199 ± 3.945 in non pregnancy group,there were statistically significant differences between the two groups(P =0.030,0.039,0.028,respectively).Conclusion Up-regulation of mir-29c,mir-200a,mir-145 in the endometrial tissue might play a role in endometriosis associated infertility.

Objective To explore the value of cardiac troponin-I (cTnI), B-type natriuretic peptide (BNP) and blood lactic acid (Lac) on evaluation of severity and prognosis in patients with septic myocardial dysfunction (SMD). Methods According to retrospective analysis of clinical data,161 cases with sepsis were divided in to SMD group and non-SMD group. And the SMD group was further divided in to death group and survival group. Blood cTnI, BNP and Lac value in each group were detected respectively. The ROC curve was used to evaluate the forecast value of cTnI, BNP and Lac on prognosis for patients. Results The value of cTnI, BNP and Lac in SMD group were significantly higher than those in non-SMD group(P<0.05);The value of cTnI, BNP and Lac in death group among the SMD patients were significantly higher than those in survival group(P<0.05);cTnI, BNP and Lac contribute to predict the 28 day mortality rate of SMD. Conclusions Blood cTnI, BNP and Lac contributes to the assessment of the severity and the prognosis of septic patients with myocardial dysfunction.