Totally 20 children with cerebral palsy of spastic type, aged 1.5-5.0 years, who were treated in Department of Children Nerve Rehabilitation, Nanhai Maternity and Kid Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, were selected from March to December 2005. Of them, 11 cases were moderate degree of cerebral palsy and 9 cases were severe. The diagnostic criterion of cerebral palsy referred to the diagnostic criterion established at the Countrywide Special Topic Proseminar of Cerebral Palsy in 2004. The method to diagnose cerebral palsy of spastic type referred to the method of Cerebral Palsy Academy of United States in 1956. Cerebral palsy was clinically characterized by the very brisk reflex of extending. The degree was graded by mild, moderate and severe according to the paralyzed part, mental development, speech development, activity of daily living and complication. The children were promoted to stand and walk by themselves mainly with standing promoting training and with restraining method as complement. The course of treatment was 6 months. The curative effect was evaluated by the abducent angle of the hip joint, the angle of popliteal fossa, the dorsiflexion angle of ankle, and the forth item of gross motor function measure, which were measured before and after the treatment. Theresult showed that the range of motion of 20 children was improved. The score of gross motor function measure of standing item improved evidently. A total of 8 cases had significant effect and 12 cases had valid effect. The total effective rate was 100%.

Objective:To study the trace injection pump in the application of the common problems and treatment measures.Methods: 246 cases of general surgery patients were used trace injection pump problems for retrospective analysis, in order to propose the improvements about engineering and management.Results: The incidence rate of non-standard operation procedures or the incidents are 6.1%, injection pump failure and infusion speed is 2.03%, mismatched injection pump attachments is 1.22% and phlebophlogosis is 0.41%. The non-standard operation procedures and imperfect trace injection pump management are the major problems. Therefore, some solutions are proposed, which includes strengthening technical training for medical staff, standard operation procedures, regular quality monitoring and healthy education to the patients.Conclusion: The operation procedures of trace injection pump should be standard and programmatic. It is of great significance to clinical application of the trace injection pump and to work properly by strengthening operation personnel quality, dealing with problems timely and correctly.

On the basis of the preliminary study of the prevention and treatment of Bohai Sea divers' dermatitis from 1983 to 1987, the morphological characteristic of 5. rosea (see section I ), the pathogenic organism of the divers' dermatitis, was studied in the field of systematic zoology, some aspect concerning medical pathogenic characteristic of the nematocysts from the red sea anemone (section I ) was studied from the point of view of pathophysiology,a preliminary study on active proteins from 5. rosea (section I ) was studied according to toxicology. The article further stated the pathogenic mechanism of the sea anemones' dermatitis, in order to go further into the new ways and methods for preventing and treating the divers' dermatitis.

Objective:To study the comparison of advantages of 3D and 2D laparoscope applied in clinical surgery.Methods: 42 cases were received retrospective analysis using 3D and 2D laparoscopic technology to complete abdominal surgery and were divided into 3D group and 2D group according to the methods of surgery. We observed the indexes for operation time, intraoperative blood loss, postoperative exhaust, catheter indwelling time, placement time of abdominal cavity drainage tube and postoperative hospitalization days, etc.Results: The two groups of patients completed smoothly laparoscopic surgery. There was no significant difference between 3D and 2D laparoscopic monitoring on indexes in the postoperative exhaust, catheter indwelling time, placement time of abdominal cavity drainage tube and postoperative hospitalization days. But the difference was statistically more significant in the operation time and intraoperative blood loss in 3D than that in 2D group (t=0.358,t=2.455;P<0.05).Conclusion:3D high definition amplification of three-dimensional surgical field similar with the traditional laparotomy is the future trend in the development of minimally invasive surgery. Compared with 2D, 3D technology can improve the overall level of the operation, with the features of easier and faster operation, smaller wound, quicker recovery, less postoperative complications, etc.

Objective To explore the effect of vibroacoustic therapy with Five Elements Music on muscle tone of children with cerebral palsy. Methods 90 children with spastic cerebral palsy were divided into three groups with 30 cases in each group, named conventional therapy group, listening group and vibroacoustic group. Conventional group was treated with physical therapy, massage and hydrotherapy by Chinese medicine. Listening group were treated by listening to the Jiao Music in addition to the conventional therapy. Vibroacoustic group was treated by vibroacoustic therapy based on the listening group. The listening group and vibroacoustic group were treated for 30 min a day for 20 d. The muscle tone and the range of motion (ROM) of hips, knees and ankle before and after treatment were assessed. Results There was no significant difference in age and gender, as well as the muscle tone in lower limbs and ROM of hips, knees and ankle among 3 groups before treatment (P>0.05). After treatment, the muscle tone decreased in the listening group and the vibroacoustic group (P<0.05).The ROM of hips, knees and ankle improved only in the vibroacoustic group (P<0.05). Conclusion Listening to the Jiao music of Five Elements and vibroacoustic therapy with Jiao music can decrease the muscle tone in lower limbs of children with spastic cerebral palsy. And vibroacoustic therapy with Five Elements Music can also improve the ROM of lower limbs the patients.

Objective:To explore the approaches and ideas for the pharmaceutical care performed by clinical pharmacists for the patients with bone tuberculosis and tubercular meningitis. Methods:Pharmacists participated in the clinical consultation for one patient with bone tuberculosis and tubercular meningitis. The methods and effect of clinical pharmacists were analyzed comprehensively in re-spect of the adjustment of anti-tuberculosis treatment scheme according to the disease condition, attention pald to the adverse reactions of anti-tuberculosis drugs, medical education for the patient and so on. Results: During the treatment, clinical pharmacists did have some actions to assist doctors in rational drug use, and the suggestions of clinical pharmacists in the consultation obtalned clinical rec-ognition, which significantly improved the effect of the medication. Conclusion:Clinical pharmacists can perform individual pharma-ceutical care for patients and help clinicians optimize drug therapy, which can improve the safety and efficacy of medication.

Objective To evaluate the therapeutic methods and the key points in the management of delayed complications of bile duct stenosis after iatrogenic bile duct injuries.Methods Five patients with bile duct injuries developed delayed complications of bile duct stenosis after cholecystectomy were retrospectively studied.All clinical information were derived from the medical data of these 5 patients treated in our department from June 2002 to July 2006.Results Of the 5 patients with delayed complications of iatrogenic bile duct injuries,2 patients developed common bile duct occlusion,2 patients developed hilar bile duct stenosis,and 1 patient developed anastomotic stenosis after cholangiojejunostomy.Bile duct injuries occurred in 3 patients after laparoscopic cholecystectomy,and in another 2 patients after mini-laparotomy-cholecystectomy.The locations of the bile duct injuries,according to Strasberg classification,were type E1 (n=1),type E2 (n=1),type E3,(n=1),and type E5 (n=2).All 5 patients received PTCD initially to relieve obstructive jaundice and then they received surgical exploration and biliary reconstruction.These patients received cholangioplasty at the porta hepatis,and hepaticojejunostomy using a Roux-en-Y anastomosis.Two patients received Roux-en-Y anastomosis of the common hepatic duct to the jejunum.All these patients had good recovery and were discharged well postoperatively.All patients had been followed-up for 7 years or more,with no evidence of obstructive jaundice or biliary tract infection.Conclusions For patients who present with delayed complications of bile duct stenosis after iatrogenic bile duct injuries,primary PTCD is carried out to relieve jaundice and to control infection.Meticulous dissection of the porta hepatis,reliable anastomosis of the proximal healthy bile duct tissues to a jejunal loop,and adequate size of anastomosis guarantee success of surgery.

International Classification of Functioning, Disability and Health issued by the World Health Organization is the international general theoretical framework for describing and measuring healthy on individual and population level, which the evaluation for cerebral palsy should follow with. This paper discussed the tools of evaluation for cerebral palsy in the view of ICF, especially the 4 dimensions of body function, body structure, activities and participation, and environmental factors.

Objective:To review the chemical and pharmacological activities of Codonopsis lanceolata in order to provide reference for the further development of C. lanceolata. Methods:The related literatures at home and abroad in the past 40 years were reviewed and analyzed, and then the chemical components and pharmacological actions of C. lanceolata were summarized. Results: The major chemical constiturents in C. lanceolata were terpenoids, alkaloids, phenylethanoid glycoside and flavonoids. The pharmacological ac-tivities were antioxidation, anti-inflammatory, anti-tumor, antiplatelet aggregation, hypoglycemic and hypolipidemic effects, etc. Con-clusion:The review provides reference for the further development and comprehensive utilization of C. lanceolata. The development of relevant safe and effective agents is still needed, and at present, the definition of mechanism and the extension of clinical application remain as the primary tasks of the exploration of C. lanceolata.

Objective To observe the effect on Foxg1 gene expression in the subgranular zone (SGZ) of cerebral tissue from neonatal rats with hypoxic-ischemic brain damage (HIBD) after transplantation of neural stem cells (NSCs) derived from umbilical cord blood.Methods Mononuclear cells separated from umbilical cord blood by density gradient centrifugation were cultured with orientated induction to differentiate the NSCs.The neuronal phenotype was identified using immunocytochemical methods.A total of 150 Sprague-Dawley rats were randomly divided into a sham-operation group,an HIBD group and an HIBD-NSCs group.Rats in the HIBD group and the HIBD-NSCs group were subject to ligation of the left carotid artery and then kept in a box under 8％ oxygen and 92％ nitrogen for 2.5 hours to establish the HIBD animal model.The artery was separated but not ligated in the sham operation group,which was not subjected to hypoxia.Twenty-four hours after the operation,the cultivated NSCs were transplanted by caudal vein injection into the rats in the HIBD-NSCs group.Rats were then sacrificed on the 3rd,7th,14th,21st and 28th days after the operation.Foxg1 gene expression in the SGZ was examined using in-situ hybridization methods.Results The number of Nestin-positive cells peaked on the 6th day of cultivation and then decreased by the 9th day.The Foxg1 gene was expressed in the SGZs of each group.The expression increased by the 3rd day after surgery in the HIBD and HIBD-NSCs groups,and peaked on 7th day after the operation,then declined gradually.The average expression level of Foxg1 in the HIBD group was significantly lower than that in the HIBD-NSCs group on the 7th day and thereafter.Conclusions Human umbilical cord blood mesenchymal stem cells can be induced and differentiated into neural stem cells.Foxg1 genes can still be present in the SGZ after birth.HIBD can induce the expression of Foxg1 genes.Transplanting NSCs can promote the expression of Foxg1 genes and improve morphological and functional recovery after HIBD,at least in neonatal rats.