Objective To explore the operative cooperation and nursing points of holmium laser lithotripsy under percutaneous nephroscope. Methods A retrospective study was carried out to investi-gate the operative cooperation and nursing of 38 patients receiving holmium laser lithotripsy under percuta-neous nephroscope in our hospital from March 2006 to August 2007. Results Stones were completely cleared in 31 patients at first holmium laser lithotripsy,in 5 patients at second holmium laser lithotripsy,in 2 patients at third holmium laser lithotripsy. One patient complicated with water intoxication and septicemia. Operation time ranged from 50 to 190 minutes,blood loss volume reached 50 to 200 ml with a average of 100 ml.No blood transfusion was needed during the operations and no secondary hemorrhage was seen postoperation. Double J catheter was inserted and indwelled for 30 to 60 days with a average of 38 days. Postoperative follow-up was satisfying. Conclusions Holmium laser lithotripsy under pereutaneous nephroscope possesses the advantages of little trauma,hemorrhage and complication and quick recovery.It is the best choice for treatment of calculus in urinary system.

Objective To discuss diagnosis and surgical treatment for chronic lymphocytic thyroiditis (CLT). Methods Records of 70 patients who were diagnosed as CLT from 2005 to 2009 were retrospectively reviewed. They were divided into two groups according to pathological results. Results There were 6 patients of CLT accompanying with papillary carcinoma. The rest 64 patients were diagnosed as CLT accompanying with benign nodules. All cases were confirmed by pathology and treated with operation. Conclusions Coexistence of nodules which can not be excluded from carcinoma is the main reason for surgical treatment in case of CLT. CLT patients especially for those associated with single nodule and/or microcalcification inside nodules should be given surgical treatment. The operative method depends on pathological results during operation.

OBJECTIVE:To observe the efficacy and safety of rebamipide triple therapy in the treatment of gastric ulcer with bleeding. METHODS:130 patients with gastric ulcer with bleeding were randomly divided into control group(65 cases)and obser-vation group(65 cases). Control group received Omeprazole enteric-coated capsule 20 mg,orally,twice a day+Aluminum magne-sium carbonate tablet 500 mg,orally,once a day;observation group was additionally received Rebamipide tablet 0.1 g,orally,3 times a day. They were treated for 12 weeks. Clinical efficacy,remission time of belching,abdominal pain,bloating,hematemesis/hematochezia,clinical symptom score before and after treatment,rebleeding and the incidence of adverse reactions in 2 groups were observed. RESULTS:The total effective rate in observation group was significantly higher than control group,remission time of symptoms and signs were significantly shorter than control group,the incidence of rebleeding was significantly lower than con-trol group,with statistical significances (P<0.05). Before treatment,there was no significant difference in the clinical symptom score (P>0.05). After treatment,the clinical symptom scores in 2 groups were significantly lower than before,and observation group was lower than control group,with statistical significances (P<0.05). And there was no significant difference in the inci-dence of adverse reactions (P>0.05). CONCLUSIONS:Rebamipide triple therapy shows obvious short-term efficacy in the treat-ment of gastric ulcer with bleeding,which can effectively improve patients'gastrointestinal symptoms,reduce rebleeding risk,and does not increase the incidence of adverse reactions.

Objective To investigate the effect of erythropoietin (EPO) on vascular endothelial cell apoptosis induced by homocysteine (Hcy) and the role of nuclear factor-κB (NF-κB) p65.Methods Human umbilical vein endothelial cells (HUVECs) were divided into normal control,Hcy treatment,EPO pretreatment,and simple EPO groups.The cell viability was detected by the methylthiazolyl tetrazolium (MTT) assay,the apoptosis rate was measured by flow cytometry,and the expression of NF-κB p65 was tested by Western blot.Results The apoptosis rates in the groups of the normal control,Hcy treatment,EPO pretreatment and simple EPO (all n =3) were 2.23 ± 0.4％,12.8 ± 1.2％,3.2 ± 0.5％ and 2.18 ± 0.6％,respectively (F =1 105.630,P =0.000).The apoptosis rate in the Hcy treatment goup was significantly higher than that in the normal control group (P =0.000) ; there was no significant differences between the simple EPO group and the normal control group (P =0.616); the apoptosis rates in the groups of EPO pretreatment (P =0.000) and simple EPO (P =0.000) were significantly lower than that in the Hcy treatment group.Western blot analysis showed that the normal control group almost did not express NF-κB p65.The expression of NF-κB p65 in the groups of Hcy treatment,simple EPO and EPO pretreatment (all n =3) were 66.1 ± 7.3,1 046.1 ± 71.3 and 1 362.4 ± 25.3,respectively.There were significant differences among the 3 groups (F =1 310.954,P =0.000).The expression of NF-κB p65 in the groups of EPO pretreatment (P =0.000) and simple EPO (P=0.000) were significantly higher than that in the HCY group.The expression of NF-κB p65 in the EPO pretreatment group was significantly higher than that in the simple EPO group (P =0.007).Conelusions EPO may attenuate Hcy-induced endothelial cell apoptosis via upregulating the expression of NF-κB p65.

The major immunogenic proteins (Ems,E2 and NS3) of classical swine fever virus (CSFV) (Shimen strain) were expressed in E.coli and purified by affinity chromatography.The recombinant antigens were applied to develop multiple enzyme-linked immunosorbent assays (ELISAs) for the detection of specific antibodies in pig sera.Optimum cut-off values were determined by receiver operating characteristic (ROC) analysis after testing 201 sera of vaccinated pigs and 64 negative sera of unvaccinated piglets.The multiple ELISAs were validated with 265 pig sera yielding high sensitivity and specificity in comparison with the virus neutralization results.The results demonstrated that multiple ELISAs can be a valuable tool for the detection of CSFV infection and serological surveys in CSFV-free countries or for the evaluation of the antibody responses in pigs induced by a live attenuated C-strain vaccination.

Good patient care requires accurate and comparable laboratory test results. To achieve standardization, a reference system consisting of a hierarchal framework of validated and reliable analytical methods linked by reference materials must be in place. Supported by national and municipal research programs, construction of reference systems has been pursued and progress been made. Candidate reference methods have been set up or developed for some important tests, and the methods have been used in the calibration or evaluation of routine methods, study of commutability of control materials and characterization of reference materials. Phenomenal roles of reference systems are expected in diagnostic reagent manufacturing, internal quality assurance and external quality assessments.

Roles of reference method in clinical biochemistry were introduced.Reference method is an important component of the reference system.Reference methods are primarily used for the establishment and assurance of the traceability of measurement results through calibrating and evaluating routine methods and assigning values to reference materials.Calibration biases and non-specificities of routine methods and the commutability of reference materials are the major issues in the standardization of clinical biochemistry.Standardization also requires availability of commutable reference materials.Reference methods are irreplaceable tools to identify the issues and to characterize reference materials.

Objective To study the diagnostic value of 3 dimentional CT reconstruction in upper urinary tract obstructive diseases. Methods 37 patients with upper urinary tract obstructive diseases underwent thin slice helical CT scan and 3 dimentional reconstruction of the urinary tract as well as 3 dimentional CT angiography when needed. Results All the cases have been definitely diagnosed including ureteral carcinoma in 8, bladder carcinoma in 2, ureteral calculi in 9, benign ureteral stricture in 7, congenital malformation in 7 and pelvic tumor in 4.All the patients except those with pelvic tumors underwent surgical intervention and the diagnosis were confirmed.The operative findings were in concord with the helical CT results. Conclusions 3 dimentional CT reconstruction of the urinary tract can provide distinct urinary tract images and helps determining the site and the cause of the obstruction. Compared with other methods, the procedure has the advantages of high resolution, rapid imaging and low invasiveness. It might become the most valuable means of detecting upper urinary tract diseases.

ObjectiveTo study the age-dependent alternations in ?-adrenergic responsiveness and the G proteins in human detrusor of urinary bladder.MethodsThe responsiveness to ?-adrenergic agonists isoprenaline and BRL37344,Forskolin and dibutyryl cyclic AMP(DBcAMP) of detrusor samples from 10 cases of senile people were examined by cellular functional test,and the influence of pertussis toxin and cholera toxin on ?-adrenergic agonists function was also studied,radioligand binding assay was performed using non-selective ?-adrenergic receptors ligand - dihydroalprenolol,the detrusor specimens of 7 young men served as controls.ResultsIn aging detrusor, there was no age-dependent change in the contractile response to KCl while the relaxing responses to isoprenaline,BRL37344 and Forskolin decreased by 15.0%,17.6% and 12.6% respectively(P

Purpose:Our experiments are dealing with the affect of docosahexaenic acid(DHA) in doxorubicin to cytoxicity effect of MDA-MB-435s, which is of the anti-human breast cancer and whether it is one of the effect mechanisms that it via the lipid peroxidation of DHA.Methods:Add different match such as anti-cancer drugs?PUFAs?oxidan system of sodium ascorbate and ?-methy-1.4-naphoqui-none?antioxidant of Dl-?-tocopherol and so on to the culture cell line of MDA-MB-435s each 24 hours for 6 days, estimate MDA by TBA with cell extracts and estimate the contents of NO via nitrite salt spectrophotography, then draw out a dosage-effect correlation straight line among contents of MDA,NO and cytoxicity. Mitox is used as contraposition to the peroxidation of ADM which is enhanced by DHA for peroxidation of mitox is low.Results:There have been obvious changes on the contents of MDA of the lipid peroxidation has paralled increased while NO has decreased. There is recitilinear correlation relationship among the contents of MDA and NO from cell extracts and the cytoxicity.Conclusions:It is obvious that DHA can add the cytoxicity of cell line of MDA-MB-435s with ADM, particularly after add non-cytoxicity oxidant. It is lipid peroxidation of oncocyte that one of the mechanisms of DHA can enhance anti-breast cancer effect of ADM.