OBJECTIVE:To observe the efficacy and safety of rebamipide triple therapy in the treatment of gastric ulcer with bleeding. METHODS:130 patients with gastric ulcer with bleeding were randomly divided into control group(65 cases)and obser-vation group(65 cases). Control group received Omeprazole enteric-coated capsule 20 mg,orally,twice a day+Aluminum magne-sium carbonate tablet 500 mg,orally,once a day;observation group was additionally received Rebamipide tablet 0.1 g,orally,3 times a day. They were treated for 12 weeks. Clinical efficacy,remission time of belching,abdominal pain,bloating,hematemesis/hematochezia,clinical symptom score before and after treatment,rebleeding and the incidence of adverse reactions in 2 groups were observed. RESULTS:The total effective rate in observation group was significantly higher than control group,remission time of symptoms and signs were significantly shorter than control group,the incidence of rebleeding was significantly lower than con-trol group,with statistical significances (P<0.05). Before treatment,there was no significant difference in the clinical symptom score (P>0.05). After treatment,the clinical symptom scores in 2 groups were significantly lower than before,and observation group was lower than control group,with statistical significances (P<0.05). And there was no significant difference in the inci-dence of adverse reactions (P>0.05). CONCLUSIONS:Rebamipide triple therapy shows obvious short-term efficacy in the treat-ment of gastric ulcer with bleeding,which can effectively improve patients'gastrointestinal symptoms,reduce rebleeding risk,and does not increase the incidence of adverse reactions.

Objective To explore the operative cooperation and nursing points of holmium laser lithotripsy under percutaneous nephroscope. Methods A retrospective study was carried out to investi-gate the operative cooperation and nursing of 38 patients receiving holmium laser lithotripsy under percuta-neous nephroscope in our hospital from March 2006 to August 2007. Results Stones were completely cleared in 31 patients at first holmium laser lithotripsy,in 5 patients at second holmium laser lithotripsy,in 2 patients at third holmium laser lithotripsy. One patient complicated with water intoxication and septicemia. Operation time ranged from 50 to 190 minutes,blood loss volume reached 50 to 200 ml with a average of 100 ml.No blood transfusion was needed during the operations and no secondary hemorrhage was seen postoperation. Double J catheter was inserted and indwelled for 30 to 60 days with a average of 38 days. Postoperative follow-up was satisfying. Conclusions Holmium laser lithotripsy under pereutaneous nephroscope possesses the advantages of little trauma,hemorrhage and complication and quick recovery.It is the best choice for treatment of calculus in urinary system.

Objective To discuss diagnosis and surgical treatment for chronic lymphocytic thyroiditis (CLT). Methods Records of 70 patients who were diagnosed as CLT from 2005 to 2009 were retrospectively reviewed. They were divided into two groups according to pathological results. Results There were 6 patients of CLT accompanying with papillary carcinoma. The rest 64 patients were diagnosed as CLT accompanying with benign nodules. All cases were confirmed by pathology and treated with operation. Conclusions Coexistence of nodules which can not be excluded from carcinoma is the main reason for surgical treatment in case of CLT. CLT patients especially for those associated with single nodule and/or microcalcification inside nodules should be given surgical treatment. The operative method depends on pathological results during operation.

Objective To investigate the factors and the preventive methods of gastrointestinal leakage.Method 32 cases of gastrointestinal leakage from 1996 to 2001 were analyzed retrospectively.Results In 32 patients,19 cases were benign diseases and 13 cases were malignant diseases.25 cases were selectively operation and 7 were emergency operation, 11 cases treated by re-operation and 21 cases by other methods.24 cases were cured and 8 cases were died.Conclusions Main cause of leakage was operative method unsuitable.Anastomostic skill was deficiency,local condition was bad and ignored postoperative treatment and so on.Preventive methods should be selected to decreasingintestinal leakage.

Objective To investigate the expressions of Bcl-2 and Bax protein in the renal tissues of patients with lupus nephritis(LN)and their role in the pathology alteration of LN.Methods During 2003-01～2003-04 immunohistochemical staining was used to detect the expressions of Bcl-2 and Bax in renal tissues of 44 cases of LN in the First Affiliated Hospital of Guangxi Medical University.Their relationship with pathology was also observed.Results (1)The expression of Bcl-2 and Bax was significantly increased and both were positively correlated with the grade of intraglomerular cell proliferation and proliferating cell nuclear antigen(PCNA)positive glomerular cells.(2)The expression of Bcl-2 in interstitial cells was significantly increased and positively correlated with the degree of interstitial infiltration of inflammatory cells.(3)The expression of Bax in the tubule of LN was increased and was positively associated with tubulointerstitial lesions.Conclusion There is abnormal expressions of Bcl-2 and Bax in renal tissues of LN.Overexpression of Bcl-2 in renal tissues of LN might play an important role in the process of glomerular hypercellularity and interstitial infiltration of inflammatory cells.Overexpression of Bax in tubular ducts is correlated with tubulointerstitial lesions.

OBJECTIVE： To prepare Yousaiqing suppository for treating ulcerative colitis and observe its clinical therapeutic effect.METHODS： The ingredients of suppository， PASA and berberine HCl， were determined with RP-HPLC and the therapeutic effect was observed in comparison with sulfasalazine suppository.RESULTS： This method of quality control was feasible and the effective rate for ulcerative colitis amounted to 70.7％ .CONCLUSION： The quality control method is simple， reliable and suitable to routine analysis and the therapeutic effect is satisfactory.

Objective To probe the feasibility, indications and efficacy of damage control orthopedics (DCO) in treating severe multiple injuries combined with fractures. Methods A retrospective analysis was done on the clinical data of 31 patients with severe multiple injuries combined with fractures treated by DCO from 1998 to 2008. Results Of all patients, 18 patients were combined with pelvic fractures and hemorrhage. Bilateralis hypogastric artery ligation was performed early in 17 patients and nonage pelvis external fixation was performed in 12. Eleven multiple trauma patients combined with femoral fractures received primary debridement and temporal external fixation for further definitive internal fixation. Two patients with spinal fractures combined with spinal cord compression received primary simple decompression. All patients received resuscitation in ICU for average 4.3 days (1-11 days). There was one death due to hemorrhagic shock. Conclusions DCO well conforms to pathophysiological character of the wounded. Early DCO and later integrated treatment are key to enhancement of the survival rate of the multiple trauma patients.

Objective To investigate the effect of erythropoietin (EPO) on vascular endothelial cell apoptosis induced by homocysteine (Hcy) and the role of nuclear factor-κB (NF-κB) p65.Methods Human umbilical vein endothelial cells (HUVECs) were divided into normal control,Hcy treatment,EPO pretreatment,and simple EPO groups.The cell viability was detected by the methylthiazolyl tetrazolium (MTT) assay,the apoptosis rate was measured by flow cytometry,and the expression of NF-κB p65 was tested by Western blot.Results The apoptosis rates in the groups of the normal control,Hcy treatment,EPO pretreatment and simple EPO (all n =3) were 2.23 ± 0.4％,12.8 ± 1.2％,3.2 ± 0.5％ and 2.18 ± 0.6％,respectively (F =1 105.630,P =0.000).The apoptosis rate in the Hcy treatment goup was significantly higher than that in the normal control group (P =0.000) ; there was no significant differences between the simple EPO group and the normal control group (P =0.616); the apoptosis rates in the groups of EPO pretreatment (P =0.000) and simple EPO (P =0.000) were significantly lower than that in the Hcy treatment group.Western blot analysis showed that the normal control group almost did not express NF-κB p65.The expression of NF-κB p65 in the groups of Hcy treatment,simple EPO and EPO pretreatment (all n =3) were 66.1 ± 7.3,1 046.1 ± 71.3 and 1 362.4 ± 25.3,respectively.There were significant differences among the 3 groups (F =1 310.954,P =0.000).The expression of NF-κB p65 in the groups of EPO pretreatment (P =0.000) and simple EPO (P=0.000) were significantly higher than that in the HCY group.The expression of NF-κB p65 in the EPO pretreatment group was significantly higher than that in the simple EPO group (P =0.007).Conelusions EPO may attenuate Hcy-induced endothelial cell apoptosis via upregulating the expression of NF-κB p65.

The major immunogenic proteins (Ems,E2 and NS3) of classical swine fever virus (CSFV) (Shimen strain) were expressed in E.coli and purified by affinity chromatography.The recombinant antigens were applied to develop multiple enzyme-linked immunosorbent assays (ELISAs) for the detection of specific antibodies in pig sera.Optimum cut-off values were determined by receiver operating characteristic (ROC) analysis after testing 201 sera of vaccinated pigs and 64 negative sera of unvaccinated piglets.The multiple ELISAs were validated with 265 pig sera yielding high sensitivity and specificity in comparison with the virus neutralization results.The results demonstrated that multiple ELISAs can be a valuable tool for the detection of CSFV infection and serological surveys in CSFV-free countries or for the evaluation of the antibody responses in pigs induced by a live attenuated C-strain vaccination.

Objective To investigate the efficacy of paracentesis using jugular vein indwelling catheter (Angiocath) in the treatment of simple renal cysts. Methods We retrospectively analyzed clinical data of 61 patients with simple renal cyst treated by Angiocath paracentesis combined with sclerosing agent injection under the guidance of ultrasonography. Results Fifty-two patients were cured on one session, while a recurrence was seen in 9 patients, among which 6 patients were cured by a re-paracentesis and 3 frequently recurrent patients were cured by the unroofing operation. No complications occurred except for 3 cases of mild transient hematuria. Conclusions Paracentesis using Angiocath in the treatment of simple renal cysts is minimally invasive, safe, effective and economical.