Transient receptor potential (TRP) is a kind of novel Ca2+ permeant channel.It is later found that TRP channels are expressed broadly in many organisms,tissues and cell types.It is involved in the regulation of sensory conduction and cell signal transduction.Further studies are required to assess which TRPC6(a member of TRPC subfamily) is associated with the cytosolic Ca2+ levels,development and progression of cancer and tumor cell cycle.TRPC6 may be regarded as new targets for the treatment of cancer.

Objective To investigate the role of interleukin-22 (IL-22) in the pathogenesis of pityriasis rosea (PR).Methods Peripheral venous blood samples were obtained from 48 patients with PR in acute stage and recovery stage and from 20 healthy human controls.Real-time fluorescence-based quantitative PCR was performed to measure the mRNA expression of IL-22 in peripheral blood mononuclear cells (PBMCs),and enzyme-linked immunosorbent assay (ELISA) to determine the level of IL-22 in sera.Results The relative expression level of IL-22 mRNA in the patients with PR in recovery stage was significantly higher than that in the healthy controls (2.89 ±0.91 vs.1.91 ± 0.60,t =3.84,P ＜ 0.05),but lower than that in the patients in acute stage (2.89 ± 0.91 vs.4.15 ±1.36,t =3.61,P ＜ 0.05).Similarly,the level of serum IL-22 was significantly increased in the patients with PR in recovery stage compared with the healthy controls ((21.44 ± 7.33) vs.(13.22 ± 4.26) ng/L,t =4.49,P ＜ 0.05),and increased in the patients with PR in acute stage compared with those in recovery stage ((36.87 ± 10.63) vs.(21.44 ± 7.33) ng/L,t =5.01,P ＜ 0.05).The level of serum IL-22 was positively correlated with the stage of PR (r =0.883,P ＜ 0.05).Conclusions IL-22 is overexpressed in PBMCs and sera of patients with PR,implying that IL-22 is involved in the pathogenesis of PR.

Objective To investigate the clinic application of venturi oxygen therapy heated humidify system on artificial airway management. Methods 30 cases of experimental group used venturi oxygen therapy for humidification and heating, while 30 cases of control group were treated with continuous wet micro-injection pump method for artificial airway care. Humidifying effects, effects of oxygen therapy, incidence of pulmonary complications were compared between the two groups. Results The humidifying effects on experimental group (26 cases) were better than control group (17 cases) (χ2=6.648,P<0.05). The irritating cough (5 cases), airway mucosa injury (1 case) and the formation of sputum crust (2 cases) in experimental group were less than 13 cases, 6 cases, 6 cases in control group (χ2=5.079, 4.043, 7.954,P<0.05). The times of sputum drainage on experimental group was (17.28 ± 5.51) times which was less than (31.22±5.24) times of control group (t=4.312, P<0.01).The effect on oxygen therapy about oxygen saturation in experimental group was 0.932 2 ±0.020 5 when sputum drainage, and 0.982 2± 0.009 4 two minutes later, which was higher than 0.916 7 ±0.011 2 and 0.957 2 ±0.013 6 of control group (t=3.431,6.276, P<0.01). Conclusions Venturi oxygen therapy heated humidify system can reduce the formation of sputum crust, reduce the times of sputum drainage, reduce hypoxia degree and shorten the duration caused by sputum crust, improve the effect of heat and humidification of patient.

BACKGROUND:Internal fixation methods for traditional proximal clavicle fractures and sternoclavicular joint dislocation include Kirschner wire, Kirschner wire with tension band, clavicular hook plate and ordinary T-shaped plate fixation. However, al of these are easy to fal off, damage nerves and blood vessels and affect the fine motion of the sternoclavicular joint. OBJECTIVE:To investigate the therapeutic effects of internal fixation with T-shaped stainless steel locking plate on proximal clavicular fractures and sternoclavicular joint dislocation as wel as to observe the biocompatibility of materials with the host. METHODS: Twelve patients with proximal clavicular fractures and sternoclavicular joint dislocation were enroled at Shanxian Central Hospital from March 2011 to January 2014, including seven cases of proximal clavicular fractures and five cases of sternoclavicular joint dislocation. Al of patients were subject to open reduction and internal fixation with T-shaped locking plate. RESULTS AND CONCLUSION:Al the 12 patients were folowed up for 3-14 months, with an average of 9 months. Al incisions healed wel, with no local eminence. No major substernal blood vessels and organ damage occurred. X-ray films showed that fractures healed without further dislocation and steel plate fracture. At the last folow-up, Rockwood scoring system showed that the excelent rate of shoulder function was 100%. These findings indicate that the internal fixation with stainless steel T-shaped locking plate is reliable and effective in the treatment of proximal clavicle fractures and sternoclavicular joint dislocation, with low risks and satisfactory outcomes, by which, patients can maximize the recovery of shoulder function.

Objective To investigate the protective effect of recombinant mouse OP 1 (rmOP 1) on renal ischemia reperfusion injury. Methods The full length open reading frame of mouse OP 1 was cloned by RT PCR. The rmOP 1 was prepared and its osteogenesis was assayed in vitro. Thirty Wistar rat models of renal ischemia reperfusion were randomly divided into 3 groups: preventive group, therapeutic group and control group. The former two groups were administered with rmOP 1 (250 ?g/kg) via the superior mesenteric vein at different time points. The renal function and structure were assessed by serum BUN, Cr, histological findings and the modified Miller's scoring. Results rmOP 1 showed osteoinductive activities in vitro. The level of serum BUN, Cr and Miller's scoring were higher in control group than the others ( P 0.05 ). Histological finding showed that rmOP 1 minimized the cell necrosis. Conclusion rmOP 1 has an protective effect on renal function and structure.

Objective To investigate the expressions of Bcl-2 and Bax protein in the renal tissues of patients with lupus nephritis(LN)and their role in the pathology alteration of LN.Methods During 2003-01～2003-04 immunohistochemical staining was used to detect the expressions of Bcl-2 and Bax in renal tissues of 44 cases of LN in the First Affiliated Hospital of Guangxi Medical University.Their relationship with pathology was also observed.Results (1)The expression of Bcl-2 and Bax was significantly increased and both were positively correlated with the grade of intraglomerular cell proliferation and proliferating cell nuclear antigen(PCNA)positive glomerular cells.(2)The expression of Bcl-2 in interstitial cells was significantly increased and positively correlated with the degree of interstitial infiltration of inflammatory cells.(3)The expression of Bax in the tubule of LN was increased and was positively associated with tubulointerstitial lesions.Conclusion There is abnormal expressions of Bcl-2 and Bax in renal tissues of LN.Overexpression of Bcl-2 in renal tissues of LN might play an important role in the process of glomerular hypercellularity and interstitial infiltration of inflammatory cells.Overexpression of Bax in tubular ducts is correlated with tubulointerstitial lesions.

With the combined interruption of the superior mesenteric vein, the splenic artery and vein and the portal vein, we successfully separated the adhesion between the collum pancreatis and the portal vein which is difficult to separate in 8 cases During the separation, the portal vein was damaged in 2～5 parts, and the size of wounds of vein were of 2～6mm in length, but the amount of bleeding was small and the bleeding speed was slow All the wounds were easily repaired under direct vision We consider that the mothod of the combined interruption is safe and effective for the separation of the adhesion between the collum pancreatis and the portal vein, and is helpful to increasing the success rate of removing carcinoma of the head of pancreases The interruption is safe for 20～25 minutes under normothermia

Objective Embryonic neuroepithelial cells (NEC) were transplanted into the brains of Parkinson disease rats, the survival and differentiation of NEC were investigated. Methods Embryonic rats (E11) were obtained from the pregnant Wistar rats. Neuroepithelial cells were dissociated from embryonic neural tube and treated with 0^25% trypsin, then transplanted them into the striatum of Parkinson disease (PD) rats stereotaxicly. Coronal sections of the brain were made in cryostat. The survival of transplant neuroepithelial cells and tyrosine hydroxylase (TH) expression of some NEP were examined by immunocytochemical technique, also the rotation behavior was tested following different time of transplantation. Results Some grafts were found survived well and some TH-positive cells could be detected in the core of graft region. NEP transplantation could also partly improve the symptoms of PD rats.Conclusion The results demonstrate that embryonic stem cells dissociated from neural tube can survive and differentiate into dopaminergic neurons. NECs contribute to the behavioral recovery.

Objective To assess the clinical value of gasless laparoscopy-assisted radical nephrectomy in the treatment of renal cell carcinoma.Methods The clinical data of 47 patients who underwent gasless laparoscopy-assisted radical nephrectomy from May 2003 to June 2005 were retrospectively analyzed.The surgical technique and its advantages and disadvantages were evaluated.Results The operative time was 75 to 135 min;and the mean blood loss was 120 to 220 ml.Postoperatively,analgesic at the dose of 5 to 35 mg(morphine sulfate equivalents) was given for pain control.The average time of indwelling drain was 2 to 3 d;the time to oral intake was 6 to 18 h;and the average hospitalization time was 5 to 7 d.Follow-up ranged from 1 month to 1 year,and all the 10 patients survived disease-free.Conclusions Gasless laparoscopy-assisted radical nephrectomy is of the advantages of both open surgery and laparoscopic surgery,and may become a good choice for the treatment of renal cell carcinoma.