Objective To observe the pulmonary artery motion curve with anatomical M-mode echocardiography, and to investigate its influential factors. Methods The pulmonary artery motion curve was detected and pulsating amplitude of pulmonary artery wall (PAWPA) was measured with anatomical M-mode echocardiography in 160 patients with normal pulmonary artery pressure. Intraclass correlation coefficient (ICC) was used to assess inter-observer conisitency of PAWPA measurements between two investigators who were blinded to each other's findings, and intra-observer consistency of PAWPA with repeating the measurements on two occasions by the same investigator in 20 randomly selected cases. Stepwise multiple regression was used to analyze the following potential influential factors of PAWPA: age, gender, heart rate, blood pressure, atherosclerosis, right atrial diameter, and pulmonary insufficiency. Results There was good consistency between two independent observers' measurements and between two occasions in the same observer's measurements for PAWPA with ICC values of 0.91 (95% CI, 0.37-0.99) and 0.95 (95% CI, 0.60-0.99), respectively. PAWPA was negatively correlated with age (β=-0.47, P<0.001) and heart rate (β=-0.29, P<0.005). There was no correlation of PAWPA with gender, blood pressure, atherosclerosis, right atrial diameter, and pulmonary insufficiency. Conclusion Anatomical M-mode echocardiography can be used for measuring PAWPA with good consistency and reproducibility. In normal pulmonary artery pressure cases, PAWPA is affected by age and heart rate.

OBJECTIVE:To evaluate the stability of cefoxitin sodium for injection compatible with metronidazole injection fluid.METHODS:Cefoxitin sodium for injection was compatible with metronidazole injection fluid and the solution was stored ordinary temperature for4hours,the maximum absorption peak and absorbance of the solution were determined by UV,and the color of the solution was observed,and its pH value was determined.RESULTS:There were no significant changes in absorp-tion peak,absorbance,color and pH value of the compatible solution.CONCLUSION:Cefoxitin sodium for injection is com-patible with metronidazole injection fluid,and the compatible solution remains stable at ordinary temperature for4hours.

Objective To investigate the prevalence of hypertension and obesity and their main influencial factors among 6～12 years old children in Shenzhen.Method 1 140 children aged from 6 to 12 years old in 4 schools in Shenzhen were sampled by random cluster sampling,and their systolic blood pressure(SBP),diastolic blood pressure(DBP),body height,weight and other morphological parameters were measured.Results The prevalent rate of hypertension was 9.4%(8.6% for boys and 10.2% for girls);Rate of overweight and obesity for boys were 13.25% and 13.72,respectively,and the girl were 9.09% and 8.10%,respectively.There were a increasing trend toward SBP and DBP with age,especially SBP.After adjusted with age and gender,the partial correlation coefficients between BMI and SBP,DBP were 0.462 and 0.357,respectively(P

Objective To observe the treatment effect of low molecular weight heparin (LMWH) in the treatment of unstable angina pectoris.Methods LMWH (0.4～0.6 ml Bid) was used through subcutaneous injection in 36 cases of unstable angina pectoris for 14 days on end.Results The clinical effective rate was 66.67%.The drug was effective in the decreasing of the frequency and time of angina pectoris,and also in the decreasing of nitroglycerin consumption as well as in the improvement of the scope and severity of cardiac ischemia by ECG.Conclusion LMWH is an effective and safe drug for the treatment of unstable angina pectoris.

Objective To discuss the methods of primarily repairing scalp,skull and dura defects of separated type D craniopagus Methods The huge defects in craniopagus was primarily repaired by the application of scalp expander, transferring scalp flap, repairing dura with pedicle periosteums, repairing skull defects with Titanium plates Results The survival baby has a good sharp of skull and well healing of scalp postoperatively Conclusions Defects of separated type D craniopagus can be primarily repaired

Objective To investigate the effects of surgical operations on different femoral condylar fractures. Methods A retrospective study was carried out on surgical operations of 84 patients with different femoral condylar fractures. The healing of fracture, functional recovery of knee joint and complications were analyzed. Results All the fractures healed in 6 to 12 months. According to Karlstrom's knee function score system, 48 knees were rated as excellent, 20 good, 13 fair, and 3 poor. The total good and excellent rate was 80.9%. Conclusions Anatomical reduction, secure fixation, timely treatment of complicated damage, and early rehabilitation exercise are important in treatment of femoral condylar fracture. The sustain blade plate is recommendable, because its design suits the anatomical structure of femoral condylar, and it not only provides convenient and stable fixation but also allows early postoperative functional training.

Objective To evaluate whether the timing of maternal hypoxia during pregnancy could myocardial remodeling in adult offspring.Methods Twenty-four pregnant rats were assigned to the maternal hypoxia group starting from the early period of pregnancy (G1,day 3 to 21 of pregnancy),the group starting from the middle period of pregnancy (G2,day 9 to 21 of pregnancy),the group starting from the late period of pregnancy (G3,day 15 to 21 of pregnancy),or the control group (G0).Six rats were included in each group.Rats in the maternal hypoxia groups (G1,G2 and G3) were subjected to hypoxia for 3 hours in a low pressure cabin with an oxygen concentration of (10± 1) ％.Systolic blood pressure,wet weight of the left ventricle normalized for body weight (left ventricular weight/body weight,LVW/BW),diameter of cardiomyocytes,and the expression levels of collagen Ⅰ and collagen Ⅲ were measured in adult male offspring at the age of 3 and 5 months.One-way ANOVA and SNK test were used for statistical analysis.Results Maternal hypoxia from day 3 to 21 of pregnancy induced higher systolic blood pressure in male offspring at the age of 3 months and 5 months [3 months:G1 (122.0± 11.7) mmHg,G0 (108.3±9.5) mmHg,F=2.97; 5 months:G1 (128.5±7.9) mmHg,G0 (114.6±-10.0) mmHg,F=3.17,both P＜0.05].Maternal hypoxia also led to a higher LVW/BW ratio (G1 1.99±0.03,G0 1.80±0.02,P＜0.05),and increased collagen Ⅰ and collagen Ⅲ expression (collagen Ⅰ /β-actin:G1 1.76±0.07,G0 0.48±0.04,P＜0.01; collagen Ⅲ /β-actin:G1 0.68±0.05,G0 0.34±0.03,P＜0.01) in adult male offspring at the age of 5 months.Maternal hypoxia from day 9 to 21 of pregnancy induced a higher LVW/BW ratio (3 months:G2 2.15 ± 0.05,G0 1.98 ± 0.02,P＜0.05; 5 months:G2 1.96±±0.05,G0 1.80±0.02,P＜0.05),and increased collagen Ⅰ and collagen Ⅲ expression (3 months:collagen Ⅰ /β-actin G2 0.98±±0.02,G0 0.87±0.02,P＜0.05; collagen Ⅲ /β-actin:G2 0.87±±0.01,G0 0.82±0.01,P＜0.05; 5 months; collagen Ⅰ /β-actin G2 1.61±0.05,G0 0.48±0.04,P＜0.01; collagen Ⅲ / β-actin:G2 0.61 ±0.04,G0 0.34±0.03,P＜0.01) in male offspring at the age of 3 months and 5 months,but did not affect systolic blood pressure.Maternal hypoxia from day 15 to 2l did not have any effect.The diameter of cardiomyocytes in male offspring was not significantly different between the groups.Conclusions Maternal hypoxia during different periods of pregnancy has different effects on cardiac structure in adult rat offspring.Maternal hypoxia started in early or mid-pregnancy leads to cardiac collagen deposition without an increase in myocyte size in adult offspring,which may be independent of the change in blood pressure.

Chronic hepatitis B(CHB)is one of the most commom cause of liver fibrosis. Accurate assessment of liver fibrosis is essential for the strategy of treatment and judgement of prognosis . Liver biopsy is the gold standard for staging fibrosis,but it is invasive with high cost,low reproducibility and poor acceptance by patients. Therefore,it is urgent to explore a noninvasive modality for the assessment of liver fibrosis. Recent evidence highlights that elastographic techniques, biochemical markers and the diagnostic model consisted of several serum markers have the potential for the diagnosis of liver fibrosis. This article reviewed the progress in noninvasive assessment of liver fibrosis in patients with CHB.

Objective To explore clinical application of retrograde medial and lateral gastrocnemius muscle flap for soft tissue defects of the middle and lower third of the leg. Methods From August 2008 to December 2009, in our hospital we adopted retrograde medial and lateral gastrocnemius muscle flap to renovate 5 cases of refractory soft tissue defects of the middle and lower third of the leg. Results Five cases of retrograde medial gastrocnemius muscle flap were survived, morphology and function of soft tissue defects were renovated well. Conclusion This operation is an effective and reliable technique for soft tissue defects of the middle and lower third of the leg, which is performed without sacrificing the major blood vessels, probing vascular pedicle and matching vascular anastomosis.

Objective To sequence the antigen-encoding genes esxA,fbpB and Rv2660c from clinical isolates of Mycobacterium tuberculosis (MTB) in tuberculosis children and study the polymorphisms.Methods A total of 135 isolated strains were amplified for gene sequences of esxA,fbpB and Rv2660c by PCR,and the products were sequenced respectively.The results of sequencing were compared with the corresponding gene sequences of standard strain H37Rv and human T and B cell epitope sequences from immune epitope database (IEDB).The genetic diversities of the gene sequences of esxA,fbpB and Rv2660c were analyzed.Results Among the 135 strains,no gene polymorphisms were found in esxA and Rv2660c.But fbpB had genetic diversities in 66 clinical isolates (48.89％).The 5 single nucleotide polymorphism (SNP) mutations in fbpB affected 8 (14.81％) T cell epitopes and 9 (24.32％) B cell epitopes.The dN and dS values of whole fbpB gene were 0.000 120 and 0.002 126,respectively.The dN and dS values of T epitopes areas were 0.000 067 and 0.002 382 respectively,those of non-T epitopes area were 0.000 408 and 0.000 470,respectively,those of B epitopes areas were 0.000 063 and 0.00 2380,and those of non-B epitopes areas were 0.Conclusion The gene sequences of esxA and Rv2660c are conservative,and that of fbpB contains genetic diversities.Its polymorphisms may influence the function of antigen Ag85B,and thus affect the efficacy of vaccine H56.