The 5-methylcytosine (~mC) in DNAs from 5-azacytidine and MNNG treated FL, Wish and Veto-E6 ceUs were analysed by HPLC. In 2?10~(-6)mol/L 5-azaCR treated cells, the percentages of ~mC in total cytosine were all lowered significantly (P 0.05). These results were in good agreement with those obtained by radioactivity analysis of newly replicated DNA fragments from Hpa Ⅱ digest. These results further validate the idea that DNA hypomethylation as a general pathway in the initiation process of chemical carcinogenesis is based on the results obtained by a defectively designed experiment.

suitable tumor markers and new targets for gene therapy of cervical carcinoma.

Objective To investigate the reasons of upgrade pathologic diagnosis after cervical operation of LEEP and the principal of treatment.Methods 101 cases of clinical data with upgrade pathological diagnosis after LEEP in our hospital during september 2005 to May 2008 were analyzed retrospectively.Results 13 cases were diagnosed as cervical intraepithelial neoplasia(CIN)Ⅰ-Ⅲ while they were considered as chronic cervicitis pre-operation.60 cases of upgrade CIN and 10 cases of in-situ cervical cancer and 10 cases of early infiltration cervical cancer and 8 cases of infiltration cervical cancer were diagnosed while they were considers as CIN Ⅰ-Ⅲ before LEEP.Among the 101 cases.34 cases were re-treated.10 cases diagnosis were the same pathological diagnosis as before,14 cases diagnosis were downgrade and 10 cases were negative.There was no upgrade pathologic diagnosis.The cutting edges were negative.And lymphatic metastasis took place in 2 cases with infiltrating cervical cancer.Conclusion The malignant ratio of patients with upgrade pathology was high.We should pay attention to the patients with upgrade pathological diagnosis after LEEP.The possible treatment should be given to them according to their age,demands of breeding,chnieal stages and types of pathologic stages.

Objective To investigate the estimated values of sequential organ failure assessment (SOFA) and quick SOFA (qSOFA) for diagnosis and prognosis in patients with sepsis according to the new diagnostic criteria in Sepsis 3.0.Methods A retrospective study was conducted.All the clinical data were collected from patients with definite diagnosis of infection and they were admitted into the Intensive Care Unit (ICU) of Beijing Traditional Chinese Medicine Hospital Affiliated to Capital Medical University from July 2014 to June 2016.The patients' gender,age,infectious location,respiratory rate (RR),oxygenation index (PaO2/FiO2),Glasgow coma scale (GCS),total bilirubin (TBil),platelet count (PLT),serum creatinine (SCr),serum lactate level,etc.general data on admission were collected to carry out SOFA and qSOFA scorings.And then the septic patients in accord with the diagnostic criteria of Sepsis 3.0 were screened out.According to outcome after admission,the septic patients were divided into survival group and death group,and the differences in diagnosis and in estimation value of prognosis between SOFA scoring and qSOFA scoring were assessed as SOFA group and qSOFA group.Results From 545 septic patients enrolled,189 septic patients consistent with the diagnostic criteria of Sepsis 3.0 were selected.In SOFA scoring group,the morbidity of septic patients was 34.68％,while in qSOFA scoring group,it was 15.96％,the difference between the two groups being statistically significant (P ＜0.01).The mortality was significantly lower in SOFA scoring group than that in qSOFA scoring group [28.04％ (53/189)vs.42.53％ (38/87),P ＜ 0.05].The mortality of qSOFA scoring group was about 1.52 times that of SOFA scoring group.On the aspect of scoring,in patients with SOFA scoring the score of death group was significantly higher than that in survival group (8.74 ± 0.417 vs.7.10 ± 0.235,P ＜ 0.01);in the patients with qSOFA scoring,the score in death group compared with that in survival group showed uo statistical significant difference (2.32 ± 0.48 vs.2.16 ± 0.37,P ＞ 0.05).On the aspect of laboratory indexes,the levels of GCS score in death group was significantly lower than that in the survival group (8.15 ± 0.67 vs.12.48 ± 0.36),blood lactate level in death group was significantly higher than that in the survival group (mmol/L:8.55 ± 4.66 vs.2.31 ± 0.16,P ＜ 0.01);the PaO2/FiO2,TBil,PLT and SCr showed no significant differences between the two groups (all P ＞ 0.05).Conclusions The new diagnostic criteria (Sepsis 3.0) can be used for diagnosis of sepsis in ICU.Compared with qSOFA scoring,the SOFA scoring is more suitable to be used for diagnosis and predicting prognosis of septic patients in ICU;SOFA scoring,GCS scoring and serum lactate level can be applied to estimate outcome of septic patients.

OBJECTIVE To share surgical experience of ossicular chain malformations with CO2 laser.METHODS A retrospective analysis was performed.9 patients with clinical diagnosis of ossicular malformations underwent ossicular reconstruction with CO2 laser-assisted from May 2010 to Mar 2016,the results were evaluated by comparing preoperative and postoperative audiometric outcomes and the rate of postoperative complications.RESULTS 8 cases with complex lesion combining incus and stapes were found intraoperatively,the deformity located on stapes was showed in one case.The mean postoperative air conduction (AC) value was (26.53 ± 12.28) dB,the mean postoperative air-bone gap (ABG) was (9.44 ± 9.62) dB,the postoperative AC and ABG value improved considerably comparing with the preoperative value in all the patients,the difference was statistically significant.CONCLUTION CO2 laser is a suitable and effective adjunct in surgery for ossicular malformations such as otosclerosis.The use of the laser improves hearing results and operation efficiency and is not likely to increase side-effects to patients.