Objective To study the clinical significance of sernm CA153,CA125 and CEA test in breast cancer.Methods The levels of CA153,CA125,carcinoembryonic antigen(CEA)and ferritin were measured in 60 patients with breast cancer(breast cancer group),36 patients with benign breast diseases(benign breast diseases group)and 40 healthy people(control group)by chemiluminometry.The four indices were compared and analyzed for their complementary diagnostic value to breast cancer.Results The levels of CA153,CA125,CEA,SF[(52.8±21.3)u/ml,(44.2±20.1)ng/ml,(8.9±5.2)ng/ml,(350.5±113.8)ng/ml]in breast cancer group were significantly higher than that of benign breast disease group[(17.3±8.8)u/ml,(15.6±8.5)u/ml,(2.0±0.8)u/ml,(1220.7±46.91)ng/ml](t=2.671,t=2.684,t=2.898,t=2.844,P＜0.01);The levels of CA153,CA125,CEA and SF pretreatment in breast cancer group were significantly higher than that after treatment[(25.5±3.7)u/ml,(15.0±8.4)u/ml,(4.6±3.3)ng/ml,(98.5±58.6)ng/ml](t=2.210,t=2.165,t=2.224,t=2.234,P＜0.05);The positive rate of 53.3%in breast cancer group for CA153+CA125+CEA+SF were lower than that CA153(56.7%),CA125(58.3%),CEA(63.3%),SF(68.3%)(χ~2=2.52,χ~2=2.652,P＞0.05;χ~2=3.85,χ~2=3.90,χ~2=3.98,P＜0.05);joint determination of CA153+CEA+SF experimental efficient 89.0%higher than the other four groups of the joint determination,but had no signiflcanle(χ~2=2.78,χ~2=3.10,χ~2=2.99,χ~2=3.01,P＞0.05).Conclusion The positive rate may be increased by combining test of serum CA153,CA125,CEA and ferritin in breast cancer.Thus the combined test might be of high value for the early diagnosis,improving the therapeutic effect and prognosis of breast cancer.

Gliomas constitute the most common type of primary brain tumor. Alternative pre-mRNA splicing may play roles in the carcinogenesis, cell growth and invasion in various types of gliomas. Factors regulating the alternative spicing in gliomas include cis-regulatory element (for example, ESE, ISS and ESS) and trans-regulatory factors (such as SRp55, SC35, SF2/ASF and PTB). Recent progresses demonstrate that many genes associated with gliomas, including those encoding tumor suppressors or promoters, enzymes, receptors and ion channels are subject to regulation by alternative pre-mRNA splicing. Therefore, studying the alternative pre-mRNA splicing in gliomas will be of benefit to understanding the molecular basis of gliomas and identifying new targets potentially useful for early diagnose as well as therapy of gliomas.

SR proteins play important roles in regulating alternative pre-mRNA splicing. As a newly discovered neural and reproductive tissue specific SR protein, SRp38 regulates the alternative splicing of several genes important for neural function, such as GluR-B, Trk-C and NCAML1. It also acts as a splicing inhibitor during mitosis or stress response in order to prevent wrong splicing. The expression of SRp38 in mouse retina was investigated by Western blot and immunohistochemistry (IHC) analyses. The result shows that the expression of SRp38 proteins in mouse retina is region-specific, with extensive distribution in the outer and inner plexiform layers, inner nuclear layer and ganglion cell layer, but no expression in outer nuclear layer. Double staining of isolated retina cells with anti-SRp38 and anti-Trk-C antibodies showed that SRp38 is localized in the dendrites, somata and axon terminals of rod-bipolar cells. By transient co-transmission of over-expressed SRp38 plasmid and RT-PCR analyses, the further results showed that overexpressed SRp38 could promote the splicing of the Flip isoform of GluR-B minegene in R28 cells. The result suggests that SRp38 may play important roles in the retinal function, possibly via regulating the neural-specific alternative splicing of genes as GluR-B.

Objective To evaluate the clinical effect of the upper lip defect with lower cross-lip flap (Abbe-Estlander flap).Methods A total of 68 cases of upper lip defect underwent the reconstruction with cross-lip flaps.We applied Abbe flap in upper lip defect in the center,and Estlander flap in lateral upper lip defect.This method was a two-stage procedure:the first stage was to rotate flap 180 degrees into the upper lip defect and to suture with the created defect of the upper lip,with careful maintenance of blood supply from the pedicle; the second was to undertake the division of bridged pedicle and paid more attention to creation of the vermilion border.Results The flaps survived in all cases.Evaluation from 3 to 12 months after the operation showed that the shape of lips were obviously repaired with excellent aesthetic and functional results.Conclusions Abbe-Estlander flap could reconstruct anatomical features and function of the lip precisely.It seems that within certain limits (probably between one-third and one-half of total upper lip length),this flap appears to be the preferred method for upper lip reconstruction.

Objective To evaluate efficacy in detecting lung nodules at low-dose CT(LDCT) by nodule enhanced viewing(NEV).Methods One hundred and twenty seven patients who were referred to undergo low-dose CT (LDCT) for the evaluation of pulmonary metastasis or screening lung cancer were selected randomly.Two radiologists with at least 10 years experience read the images with normal clinical reading speed to find actionable nodules ≤ 2.0 cm in maximum diameter,and their consensus result was referred as Standard.NEV was adopted to detect the pulmonary nodules.Two residents with experience of less than three years read first detected suspicious nodules and recorded reading time,first consensus and mean time were recorded.Then,they made second decisions on the images with the help of NEV and the results and the reading time were recorded and analyzed by using wilcoxon test.The sensitivity and accuracy of NEV,residents and residents with NEV were analyzed.Results Standard,resident,NEV and resident with NEV detected 570,404,768 and 593 lung nodules ≤2.0 cm in maximum diameter,respectively.More than 60％ nodules were less than 0.5 cm in maximum diameter.The performance of NEV in detecting nodules ≤2.0 cm as well as nodules ＜ 0.5 cm in maximum diameter was significantly higher than that of the resident(Z =-6.887,P ＜0.01 and Z =-7.235,P ＜0.01),and the performance of resident with NEV indetecting nodules ≤2.0 cm as well as nodules ＜ 0.5 cm in maximum diameter was significantly higher than that of resident without NEV (Z =-6.606,P ＜ 0.01 and Z =-6.657,P ＜ 0.01).The resident,NEV and the resident with NEV detected nodules ＜ 20 mm in maximum diameter with sensitivities of 61.4％,86.3％ and 95.3％,and with accuracy of 56.1％,58.1％ and 87.6％,respectively.The resident achieved sensitivities of 51.4％,88.1％ and 94.8％,and accuracy of 47.0％,56.9％ and 87.5％ for nodules ＜5 mm in maximum diameter,respectively.The resident,NEV and resident with NEV spent 120-444 s,85-262 s and 131-1512 s per case to read the CT scans,respectively.The reading time of resident with NEV in was significantly higher than that of resident without NEV(Z =-9.781,P ＜ 0.01).The resident spent 23 s per NEV mark.Conclusion NEV considerable improves the resident's performance in lung nodule detection,especially in maximum diameter ＜ 0.5 cm nodule detection.

Objective To discuss the clinical efficiency of transcatheter arterial chemoperfusion or chemoembolization (TACP / TACE) for treatment of liver metastasis from malignant insulinoma. Methods 9 cases of liver metastasis from malignant insulinoma were performed with TACP protocol of 5-fluorouracil,epirubicin,mitomycin C and interleukin-2,including 2 patients also received TACE with total 2-8 courses of treatment. Results All patients finished the interventional therapy uneventfully with no serious complication. After treatment,clinical symptoms disappeared or improved significantly,with obvious response in 2 cases and partial response in 7 cases. Efficacy in imaging revealed obvious response in 1 case,partial response in 7 and no response in 1 case. Conclusion TACP / TACE for treatment of liver metastasis from malignant insulinoma is safe and effective.

Objectives:The screening examination of bladder cancer by detecting urinary survivin and the survivin expression in bladder cancer were evaluated. Methods:Urinary survivin was detected by ELISA and survivn expression of tumor tissue was observed by immunohistochemistry.(Results):There was significant difference in urinary survivin between the groups of treatment and control((P

Objective To explore the value of MRI and iron oxide particle labeling in stem cell therapy of stroke model. Methods Nine rats with middle cerebral artery occlusion were randomly selected and underwent Neurological Severity Scoring(NSS), MRI and pathological examination. The results of the 3 evaluation criteria were correlated. Bone marrow stromal cells were labeled with superparamagnetic iron oxide particles. Eighteen models were screened and divided into 3 groups based on different transplantation sites. MRI was performed at different time points. The MR appearance of labeled stem cell transplantation sites was observed. The relative infarct volume of the models in three groups were recorded and compared. Results Significant correlations among the NSS, MRI and pathological examination were found. Different MR sequences could depict local transplanted labeled stem cells and gradient echo sequence was the most sensitive method, while the T2WI showed its advantage of better temporal resolution. MR images showed the morphological changes of transplanted stem cells. The change of the relative infarct volume showed no significant differences among the three groups. Conclusion MRI is an ideal tool to evaluate the rat stroke model. MRI together with iron oxide particle labeling technique helps to in vivo track and monitor the transplanted stem cells.

Objective To evaluate the feasibility and safety of transrudial cerebral angiography and to discuss the technique points, advantages, disadvantages, indication and contraindication. Methods Transradial cerebral angiography was performed in 52 patients who were not suitable for transfemoral access or could not suffer from being kept in bed for 24 hours after transfemoral angingraphy, related comphcations and success rate of puncture and selective angiography were recorded and compared with transfemoral group (n = 83 ). Chi-square test was used for statistics. Results The success rate of puncture and selective angiography and the comphcation rate of transradial and transfemoral groups were 96.2% (50/52),94.2% (49/52),9.6% (5/52) and 100% (83/83),95.2% (79/83),8.4% (7/83),respectively. There were no significant differences between two groups (X2 = 0.000, P>0.05). After angiography, 2 patients of transradial group developed a mild bruise at the puncture site, no hematoma,pseudoaneurysm and artery- venous fistula occurred. Conclusion Transradial selective cerebral angiography is technically feasible and safe with comfortable and minimal comphcations. It should be used as the choice of mutes for selective cerebral angiography.

Objective To investigate the diagnostic value of serum ferritin in children with systemic onset juvenile idiopathic arthritis (SO-JIA). Methods Fifty-seven patients with fever of unknown origin (rectal temperature>38.5 ℃ ) over two weeks and hospitalized in our general medicine ward longer than one week were enrolled in this study. Patients were recorded the course of fever, elinieal symptoms and signs including rash and swollen joints/arthralgia, laboratory tests including complete blood cell count, C-reactive protein, erythroeyte sedimentation rate, lactate dehydrogenase and the level of serum ferritin. SPSS 10.0 was used for statistical analysis. Results Two of 57 patients could not be diagnosed before discharge. The other 55 patients whose diagnosis was confirmed were divided into four groups. Twenty-five patients were SO-JIA,12 patients had hematologic or oncological diseases, 12 patients had infectious diseases and 6 patients had other rheumatic diseases. The level of serum ferritin was significantly higher (P<0.01) in SO-JIA group than in other groups. Moreover, the levels of serum ferritin in SO-JIA group were all higher than normal and the levels of serum ferritin in 76% of SO-JIA group were more than five-fold elevation. Four cut-off levels of serum ferritin level for the diagnosis of SO-JIA were selected based on clinical practice and ROC curve. When the cut-off levels of serum ferritin were 328.25, 529.50, 731.05 ng/ml and 1121.10 ng/ml, the sensitivity for the diagnosis were 100%, 88%, 72%, and 64% respectively, the specificity were 77%, 87%, 90% and 100%,respectively. Conclusion Serum ferritin is valuable for the diagnosis of SO-JIA and 529.50 ng/ml may be a good cut-off level