The liver plays an vital role in glucose and lipid metabolism,synthesis of plasma proteins,and detoxification of xenobiotics.Liver chronic disease is one of the leading causes of death in China.Liver mass can be restored by two mechanisms:division of hepatocytes and hepatic oval cells (HOCs) proliferation and differentiation.However,the origin,specific markers and signaling pathways of HOCs have not been fully elucidated.Recent researches in HOCs isolation methods and genetic lineage tracing have enabled investigators to study multiple aspects of HOCs origin and biology.We reviewed the previous researches in detail.

BACKGROUND: Cervical vertebra corpectomy decompression is one of the commonly used methods of anterior cervical decompression. In recent years, under the guidance of the principle of anterior cervical surgery, scholars propose the anterior cervical vertebra corpectomy decompression with preserving the posterior wall of the corpectomied vertebral body. It retains the posterior wall of the vertebral body, not only increases the stability of the cervical spine, but also increases the area of bone graft, which is conducive to the long-term fusion. At the same time, retaining posterior wall of the vertebral body can effectively prevent bone- and implant-induced spinal cord injury.OBJECTIVE: To evaluate the biomechanical stability of anterior cervical vertebra corpectomy decompression with preserving the posterior wall of the corpectomied vertebral body procedure on sheep by the establishment of finite element model with CT data.METHODS: Cervical vertebra specimens of adult sheep were selected, and scanned to capture its CT data. The geometrical model captured by CT was then transformed into finite element model through finite element software (without surgery). In the posterior wall preserving group, C4 corpectomy decompression was conducted while preserving its posterior wall followed by titanium-meshes and AO plate fixation. On the basis of posterior wall preserving group,corpectomy decompression was operated without preserving the posterior wall in another group; titanium mesh and plate were fixed. Finite element software was used to test and analyze the changes in stress and displacement of cervical vertebra under different conditions.RESULTS AND CONCLUSION: (1) The displacement was slightly small, and immediate postoperative stability was good in the posterior wall preserving group. However, the displacement and stress were not significantly different between posterior wall preserving group and posterior wall non-preserving group. (2) To sum up, a procedure preserving posterior wall of the vertebra body gains better immediate postoperative stability compared with conventional method.

Objective To compare new type streamlined liner pharynx airway with standard type Ⅰ laryngeal mask airway in the clinical effectiveness in gynecologic laparoscopic surgery.Methods Seventy three cases undergone gynecologic laparoscopic surgery were divided into two groups randomly.The observation group(40 cases) and the control group(33 cases) were maintained by streamlined liner pharynx airway(SLIPA) and standard type Ⅰ laryngeal mask airway respectively.The success rate of placement,throat hemorrhage and pain before/after anesthesia and other indicators.were strictly observed and recorded during anesthesia.Results The success rate of placement was significantly higher in the observation group than in the control group(P＜0.05).The incidence of throat hemorrhage and throat pain after surgery were significantly lower in the observation group(P＜0.05).Conclusion The streamlined liner pharynx airway could be easyly and safely placed and causes less tissue injury and complications in gynecologic laparoscopic surgery.SLIPA was worthy of being recommended in clinical surgery.

Objective:To explore the safety and efficacy of Castor branched stent-graft exclusion in the treatment of aortic dissection and aneurysm involving left subclavian artery.Methods:The clinical and imaging data of 88 patients with aortic dissection or aneurysm involving left subclavian artery diagnosed by CTA or DSA in our hospital from December 2017 to December 2019 were collected retrospectively, including 67 aorta dissections, 7 thoracic aortic aneurysms and 14 aorta penetrating ulcer. All patients were treated with branched stent-graft under the guidance of DSA. The postoperative curative effect and complications were observed. The paired t test was used to compare the maximum aortic diameter of the lesion before and 6 months after the operation. Results:Eighty-eight patients were successfully treated with integrated stent, the success rate of operation was 100%, and the disease-related symptoms of all patients were basically or completely relieved. The mortality rate within 1 month after the operation was 2.7% (2/88). The two deaths were complicated with other serious diseases before the operation, and the cause of death was not related the operation. All patients were followed up except 4 patients who lost contact after discharge. During the follow-up, there were 1 case of retrograde type A dissection, 1 case of new aneurysm of aortic arch, 2 cases of in-stent stenosis of left subclavian artery branch, 3 cases of mild stroke, no persistent endoleak and no death or other serious complications. The mean maximum aortic diameter at 6 months after operation [(34±4)mm] was significantly lower than that before operation [(38±6 mm)] ( t=6.63, P<0.05). Conclusion:Castor branched stent-graft is simple, mini-invasive and effective in the treatment of aortic dissection and aneurysms involving the left subclavian artery.

Objective To design a temperature control strategy for wave bioreactors.Methods According to the requirements of temperature control precision and response speed of wave bioreactors,the traditional PID control method was combined with fuzzy control method which was used to adjust the parameters of the PID control in real time online in order to strengthen the ability of the temperature control strategy to regulate temperature.Results A fuzzy PID controller was completed and simulation results were compared with the traditional PID controller.Conclusion The fuzzy PID control method has a smaller overshoot and shorter stability than the traditional one, so it has a higher temperature control performance.

Objective To assess the histological characteristics of cervical cancer using intravoxel incoherent motion magnetic resonance imaging ( IVIMMRI) and to investigate the performance of IVIMMRI in evaluation of the efficacy of concurrent chemoradiotherapy in the treatment of intermediate/advanced cervical cancer. Methods Pelvic MRI scans, containing T2WI, IVIM (14 b values, b=0?1 000 s/mm2), and contrast?enhanced T1 scans were performed in 23 patients pathologically diagnosed with intermediate/advanced cervical cancer ( stage ≥Ⅱb ) before chemoradiotherapy, after two and four weeks of treatment, and at the end of treatment. The IVIMMRI data were processed with the bi?exponential model to generate three parameters, containing pure diffusion coefficient ( D ) , pseudodiffusion coefficient ( D?) , and perfusion fraction ( f ) . Apparent diffusion coefficient ( ADC ) was obtained using the mono?exponential model. The IVIMMRI parameters were measured at each time point and their dynamics and correlation were analyzed. Results The ADC, D, and f values were significantly higher after complete treatment ( 0?96 × 10-3 vs. 1?77 × 10-3 mm2/s, P=0?000;0?76 × 10-3 vs. 1.34± 0?12 × 10-3 mm2/s, P=0?000;0?14% vs. 0?24%, P=0?012). The above three values significantly increased after two weeks of treatment (all P=0?000) and kept increasing until the end of the treatment. In contrast, the D? value was reduced from the second week to the end of the treatment. Conclusions IVIMMRI can monitor the dynamic functional changes and early tumor responses during chemoradiotherapy for cervical cancer, which holds promise for clinical application.

Objective To discuss the clinical presentations and imaging features of a novel avianorigin influenza (H7N9).Methods Anteroposterior chest radiographs and serial thin-section CT images in 5 patients with H7N9 were reviewed retrospectively by two radiologists,the predominant inaging patterns of lung abnormalities and clinical data were recorded and analyzed.Results The large consolidation of the right lung with air bronchograms was the most common radiographic appearance in all patients with H7N9 at admission,and the abnormalities were more severe in the bilateral lower lobes.Both lungs developed large,scattered consolidations and ground-glass opacities with the progression of disease.The lesions in the left lung were more rapidly absorbed than those in the right lung.Conclusion Influenza H7N9 has certain chest imaging characteristics in the onset,progression and recovery of disease.

Objective To design a temperature control strategy for riptide bioreactor to eliminate integral saturation by conventional proportion integration differentiation (PID) control.Methods According to the requirement of the riptide bioreactor for the temperature control,the temperature control system model determined by experiment was got,then the effectiveness of the integral limiter PID control method was verified,and finally the integral limiter PID control method wasimproved further using the integral separation combined with the actual experimental results and its effectiveness was tested.Results The simulation results showed that the control effects of the integral limiter PID was good.However,the actual tests proved that there was still deficiencies in large overshoot and long stable time,and good experimental results were obtainedafter improving the integral limiter PID by the integral separation method.Conclusion The improved integral limiter PID control method effectively avoids the overshoot of the system caused by the integral saturation,achieves high control precision,has a very good control performance for the temperature control of riptide bioreactor,and well meets the requirements of mammalian cell culture.

Objective To compare and analyze the supply of blood collection and clinical blood demand in Hangzhou during 2011-2016,and to put forward some countermeasures and suggestions.Methods The related data of blood collection in blood center and the indexs of clinical blood demand in all hospitals in Hangzhou were collected during 2011-2016,and the growth rates of both of them were compared and analyzed.Results 1) The data of blood collection and supply was the lowest in 2012,and then increased year by year.The average annual growth of platelet collection and supply was 8.09％ and 8.47％,respectively,and the other indicators grew relatively gently.In 2016,the rate of blood donation reached 18.28 per thousand people.At the same period,the number of staff in institutions was basically stable.2) During 2011-2016,the blood demand of all hospitals in Hangzhoa maintained rapid growth.In Hangzhou,the number of hospitals increased by 10.65％ annually,and until 2016,there was an increase of 65.87％ over 2011.The average annual growth of the number of beds,the number of emergency patients and the number of inpatients increased by 10.21％,6.09％ and 11.40％ respectively.The growth rate of number of inpatients was higher than that of outpatient and emergency departments.Hospital employees remained at an average annual growth rate of nearly 10％.3) The clinical demand for blood increased significantly more higher than the growth of blood collection and supply.Conclusion Speed up the pace of the construction of blood supply,and keep pace with the construction of hospitals.Strengthening the publicity,health education and promotion models,in order to encourage more people,who are eligible for blood donation,to join the blood donation.And also strengthening personnel team building,improving overall work efficiency and level.

Objective To explore the enrichment technique of Schistosoma japonicum cercariae on the water surface,so as to establish a new method combined with the existing technology to detect the cercarial infested water body quickly and sensitive-ly. Methods Soybean oil,gasoline,kerosene and isophorone were screened as expanding agents. The cercariae were enriched by the thrust of the expanding agents when diffusing on the water surface,and PE adsorption film and C-6 film were applied to seize them so as to determine the infectivity of the water quickly. The relationship between the dose of expanding agents and dif-fusion radius were explored. Results Gasoline,kerosene and isophorone were suitable expanding agents,and the diffusion ef-fect of isophorone was the best. After the enrichment by the expanding agents,the detection rate of cercariae of the method seiz-ing cercariae with the film significantly improved in the water. Conclusion This new method could effectively improve the de-tection rate of the cercarial infested water and is suitable for the low-degree infested water.