0.05).Conclusion Our findings suggest that patters of gene expression in the lung adenocarcinoma stem cell and normal lung stem cell are obviously different.

Objective To explore the MSCT and MRI characteristics of hydrosalpinx.Methods The CT and MRI findings of 28 patients with hydrosalpinx confirmed by surgery and pathology were retrospectively studied.Before operation,the plain and contrast enhanced CT in 25 patients and the plain and contrast enhanced MRI in 3 had been carried out.Results 1 9 patients with unilateral hydrosalpinx (12 on the left and 7 on the right side)and 9 patients with bilateral hydrosalpinx were found.Both CT and MRI images showed the cystic mass with different forms in annex area,including botuliform changes in 1 6 patients,beaded form in 9,polycystic changes in 8 and retort-like changes in 4.The low density as water on plain CT,and the low signal on T1 WI and high signal on T2 WI were showed.The cystic walls were significantly enhanced without nodules.If there is septum,mild enhancement was also found.14 patients with chronic salpingitis,2 with fascial thickening,3 with uterine fibroid and 1 7 with pelvic fluid.Conclusion CT and MRI findings of hydrosalpinx are characteristic.

Objective To improve the accuracy of diagnosis of the greater omentum blunt trauma(GOBT)by analyzing the MSCT findings and pathological features.Methods The MSCT appearances of 5 1 cases of GOBT with operation and (or)pathological con-firmed were analyzed retrospectively.All patients were underwent MSCT plain scan in whole abdomen.Two experienced radiolo-gists blinded to the clinical diagnosis,reached a diagnosis from the MSCT findings by two ways of plain and plain joint post-process-ing MSCT images.The detection rates of GOBT were compared by two-related-samples Fisher exact probability chi-square test.Re-sults Fifty-one cases of GOBT were distributed over the intestine,in the superior abdominal cavity,the left and the right abdominal cavity,those cases were 27,18,3 and 3 respectively.The MSCT findings of GOBT mainly consist of greater omentum(GO)streak-ing,GO hematoma and fluid around GO.Those detectable rates were 80.3%,68.6%and 90.2 % respectively.The accuracy in di-agnosis of GOBT with MSCT was 94.1%(48/51)in our study.The detection rate of GOBT by two ways was significant (P=0.04).The detection rate of GOBT was increased obviously by the way of plain joint post-processing MSCT images.Conclusion MSCT manifestations of GOBT has some characteristics.A more accurate diagnosis of GOBT could be made according to the MSCT findings by plain joint post-processing MSCT images.

ObjectiveTo prospectively investigate the feasibility of contrast material-enhanced magnetic resonance angiography(CE-MRA)for visualization of the spinal vasculature.MethodsTen volunteers and fifteen patients with thoracic or lumbar disk herniation underwent contrast-enhanced MR angiography of the thoracoabdominal aorta and posterior branches.Examination Was performed with three-dimensional CE-MRA technique and 0.3 mmol/kg of gadolinium contrast agent Was administrated.ResultsIn all examinations,the Adamkiewicz artery,the anterior spinal artery(ASA),and their connections witll the aorta were identified.The Adamkiewicz artery originated between T8 and L3 in all examinations and derived from the left side of the aorta in 80% of all examinations.ASA was able to be observed from T6 to L3.The diameter of Adamkiewicz artery Was 0.6-1.2 mm and ASA was 0.6-1.0 mm.Conclusion CE-MRA Can be used to lOCalize Adamkiewicz artery and provide accurate information for clinical approach.

OBJECTIVE: To explore the association between low flow rate and reperfusion injury during the process of preserving liver by cold machine perfusing perfluorochemical oxygen carder solution. METHODS: Forty-four male adult Wister rats were randomly divided into normal, control, experimental Ⅰ and experimental Ⅱ groups. In the normal group, the removed liver was performed isolated reperfusion guided by Clavien method. In the other 3 groups, the removed liver was infused through the portal vein by the 4-5 ℃ conserved perfluorochemical oxygen carder solution. The infused rate was controlled at 0.4, 0.2, 0.1 mL/(min·g) with 18 hours perfusion, After that, isolated reperfusion was performed. The activity of aspartate aminotransferase, alanine transaminase and endotheiin mass concentration of the effluent was detected at minutes 10, 30, and 60 after reperfusion. The histopathological changes of liver under light and election microscopy were also observed. RESULTS: The activity of aspartate aminotransferase, alanine transaminase and endothelin mass concentration had no remarkable differences between the experimental Ⅰ group and control group (P > 0.05). The differences between the experimental Ⅱ group and control group were remarkable (P < 0.05). The light and electron microscopy showed that the histopathological changes of liver in the control and experimental Ⅰ groups was lightener than experimental Ⅱ group. CONCLUSION: During the process of preserving liver by cold machine perfusion, the rate of 0.1 ml/(min·g) perfluorochemical oxygen carder solution increase the injury of hepatocyte and sinusoidal endothelial cells, which eventually result in severity of reperfusion injury.

Objective To explore the effect of ω-3 fish oil on nutrition improvement and inflammatory reaction of patients with gastrointestinal tumor after operation.Methods 60 cases with gastrointestinal tumor were divided into control group(30 cases) and study group (30 cases),both groups were provided with parenteral nutrition treatment[ 104.6 kJ · kg-1 · d-1 ].Fish-oil fatty emulsion was given to the study group.The postoperative for the first day received half of the total energy and the total energy The remaining four days.Blood samples were gained on the morning of day 1,on the morning of day 3 and day 6 after operation respectively to measure albumin ( ALB),prealbumin (PA),total protein(TP),transferrin(TRF),the neutrophilic granulocyte count,lymphocyte count (TLC),serum C-reactive protein(CRP).Results Both groups of patients was comparable(all P ＜ 0.05 ).Both groups of patients was treated after five days of postoperative and ALB,TP,TRF were not significantly different.PA in both groups on day 6 was significantly increased,and the study group was higher than control group.there were statistical differences between them ( all P ＜ 0.05 ).The neutrophilic granulocyte count and CRP of both groups were significant reduced,and the study group was lower than control group.There were statistical differences between them( all P ＜0.05 ).Conclusion ω-3 fish oil on nutrition could improve nutritional quality and modulate inflammatory reaction of patients with gastrointestinal tumor after operation.

Objective To investigate the effect of Livin expression on VSV-induced apoptsis of A549 cells.Methods The expression of Livin of A549 cells was inhibited by RNA interference.VSV-induced apoptosis of A549 cells was observed by Tunel assay.Protein Level of livin was detected by Western blot.Caspase-3 activity was detected by the fluorescence-based quantitative method.Results Livin downregulation VSV-induced apoptosis of A549 cells.Inhibited the expression of Livin of A549 cells had increased Caspase-3 activity.Conclusion The effect of Livin on VSV-induced apoptotic of A549 cells could be increased by RNA interference.

Objective To establish the animal model of experimental allergic encephalomyelitis(EAE)in Lewis rats.Methods The EAE was induced by giving hypodermal injections in feet with spinal cords homogenate of guinea pigs(GPSCH)and complete Freund's adjuvant(CFA)containing 8mg.ml-1 bacille calmette guerin(BCG).The EAE model was evaluated by clinical manifestations and pathologic findings which was studied with the aid of light and electron microscope.Results The EAE in Lewis rats had an incidence of 9/10,a latency of 12.6?1.01d.Bordetella pertussis vaccine(BPV)could increase the morbidity,shorten the latency(P

Objective To evaluate the feasibility and clinical value of laparoscopic partial splenectomy to treat benign splenic diseases.Methods The clinical data of 13 patients who underwent laparoscopic partial splenectomy carried out by a single operating surgeon from June 2010 to January 2016 in our hospital were analyzed retrospectively.The enrolled patients included 6 with a splenic epidermoid cyst,4 with a splenic pseudocyst and 3 with splenic hemangioma.The lesion diameters ranged from 5.1 to 12.4 cm,with an average of (7.2 ± 2.3) cm.Results The operations were all successful.There was no conversion to laparotomy and there was no perioperative death.The operations included 4 upper pole splenic resection,5 lower pole resection,2 middle and upper pole resection and 2 middle and lower pole resection.The operative time ranged from 95 to 155 min,with an average of (119 ± 17) min.The volume of intra-operative blood loss ranged from 100 to 350 ml,with an average of (187 ± 78) ml.There was no postoperative bleeding,pancreatic fistula,digestive fistula,intra-abdominal infection,splenic vein thrombosis and other complications after the operation.The average postoperative hospitalization stay was (5 ± 1) d.Conclusions In carefully selected patients,laparoscopic partial splenectomy carried out by experienced surgeons to treat benign splenic diseases is safe and feasible.

Objective To determine whether the incidence of pseudolesions of the liver caused by falciform ligament was correlated with liver function.Methods MRI of 150 consecutive cases were evaluated for the presence of pseudolesions caused by ligament compression.All cases were divided into two groups according to the Child-Pugh classification of liver function: Group I-normal liver function and Grade A(98 cases);Group II-Grades B and C(52 cases).Results The incidence of pseudolesions was 31.63% in groups I and no artifacts in Group II.The incidence of pseudolesions was significantly higher in Group I compared with those in Group II.Conclusion The artifacts in the liver caused by falciform compression on MR contrast-enhanced scan images may be seen more frequently in the patients with well liver function than in those with impaired function.