It hopes to offer help for acupuncture teaching and sci-research by summing up 10 aspects of studying space pattern of acupoints structure and risk acupoints depth,acupoints form solid structure,acupoints inclusion—immunohistochemical and molecular biology of multi-enzyme,acupoints CT scanning picture anatomy,weak irradiance path of channels,acupoints structure,acupoints acupuncture principle and clinical application,acupuncture acupoints medical engineering,and setting up Chinese characteristic series of new anatomy disciplines.

Objective:To explore the application of data mining in the literature study on acupuncture treatment Of headache,and to summarize the laws between TCM pattern identification of headache and acupoint selection,acupoint combination.Methods:In the study of the clinical literature about acupuncture treatment for headache,published domestically from 1950 to 2008,the number and frequency of the acupoints in the literature,and the selecting laws and frequency of the main acupoints in pattern identification,and the meridian-tropism relationship between the main acupoints and combined acupoints,and the distributing rules of commonly used acupoints on the body surface were investigated by frequency-analyzing method.Resuits:The main acupoints in acupuncture treatment of headache ale primarily the acupoints on the head and specific acupoints from yang meridians,with the acupoints combined in accordance with pattern identification by meridian theory for different paaerns.Conclusion:By analyzing the laws of acupoint selection in the acupuncture treatment of headache with data mining,its new laws can be obtained.

OBJECTIVE:To investigate the situation of adverse drug reactions(ADR) in our hospital.METHODS:In this retrospective study,a total of 270 ADR cases reported between 2005 and 2007 in our hospital were analyzed statistically in respect of the age and sex of patients,drug categories and clinical manifestations etc.RESULTS:Of the 270 ADR cases,patients aged ≤18 years or ≥50 years took the highest proportion(51.1% for the two age groups).The ADR were chiefly manifested as lesion of skin and its accessories,followed by the reaction of digestive system.90.74% of the ADR cases were induced by injections,and 191 cases(70.74%) were induced by antibiotics.CONCLUSION:ADR worth the highest alert in the clinic.

Objective To explore the MSCT and MRI characteristics of hydrosalpinx.Methods The CT and MRI findings of 28 patients with hydrosalpinx confirmed by surgery and pathology were retrospectively studied.Before operation,the plain and contrast enhanced CT in 25 patients and the plain and contrast enhanced MRI in 3 had been carried out.Results 1 9 patients with unilateral hydrosalpinx (12 on the left and 7 on the right side)and 9 patients with bilateral hydrosalpinx were found.Both CT and MRI images showed the cystic mass with different forms in annex area,including botuliform changes in 1 6 patients,beaded form in 9,polycystic changes in 8 and retort-like changes in 4.The low density as water on plain CT,and the low signal on T1 WI and high signal on T2 WI were showed.The cystic walls were significantly enhanced without nodules.If there is septum,mild enhancement was also found.14 patients with chronic salpingitis,2 with fascial thickening,3 with uterine fibroid and 1 7 with pelvic fluid.Conclusion CT and MRI findings of hydrosalpinx are characteristic.

To study the effect of the electroacupuncture and Buyang Huanwu Decoction (BYHWD) on the regeneration of the rat's injured sciatic nerve.

Objective To evaluate MR imaging of peripheral nerve tumors of the lower extremity. Methods MR imaging of peripheral nerve tumors of the lower extremity in five cases proved by surgery and pathology were retrospectively reviewed. In all patients, routine scanning was performed in axial, coronal, and sagittal planes, including T 1-weighted and T 2-weighted images. In two patients (schwannomas), T 1WI were obtained following intravenous injection of Gd-DTPA. Results There were four schwannomas (three benign and one malignant) and one malignant neurofibroma. Tumors arose at the following sites: leg ( n =2), popliteal region ( n =1), thigh ( n =1), and femoral region ( n =1). On T 1WI, tumors generally showed isointensity (two benign schwannomas) or lower-medium signal intensity to adjacent muscle with minimal inhomogeneity (one benign schwannoma, one malignant schwannoma, and one malignant neurofibroma). On T 2WI, tumors demonstrated inhomogeneous high signal intensity in all five patients. The target sign with peripheral hyperintense rim and central low intensity was see in two benign schwannomas on T 2WI. Conclusion MRI is useful in defining the location and extent of a lesion and in assisting the surgical planning. The target pattern appears to be a useful sign in the diagnosis of peripheral nerve tumors.

Objective To evaluate MR imaging of transient osteoporosis.Methods MR imaging of transient osteoporosis in eight patients was retrospectively reviewed.In all eight patients,routine scanning was performed in axial and coronal planes,including T_1-weighted and T_2-wighted images.Of the eight patients,five were male and three were female,with the age ranging from 12 to 70 years.Neither of the women was pregnant when they visited our hospital.Results The bilateral hips were affected in seven patients,the left shoulder in one.The MR images demonstrated low signal intensity in all eight patients on T_1WI,and normal signal intensity (2 cases),medium-high signal intensity (3 cases),or high signal intensity (3 cases) on T_2WI.The bone marrow edema (BME) pattern involved the acetabulum (one hip),both the femoral head in 5 hips,the femoral neck and the intertrochanteric region through the upper femur in 7 hips,and the upper humerus in one.A small joint effusion was observed in six hips on T_2WI.Conclusion MRI is useful in defining the location and extent of transient osteoporosis.

BACKGROUND: The complicated localization of intramedullary nails and osteotomy more dependent on surgeons' experience limit the application of conventional total knee arthroplasty (TKA). The occurrence of three-dimensional (3D) printing technology can achieve precise localization and osteotomy in TKA.OBJECTIVE: To explore the effectiveness of 3D printing technology-aided TKA versus conventional TKA for genu varum.METHODS: Thirty-four patients with genu varum undergoing primary unilateral TKA were recruited and were then divided into two groups (n=17 per group) in accordance with the random number table. One group was treated with TKA with 3D printing guild plate (3D printing group), while the other group received the conventional TKA (conventional group).The intraoperative and postoperative blood loss, operation time, as well as the Hospital for Special Surgery score, range of motion, and lower limb mechanical alignment at 2 weeks postoperatively were compared between two groups.RESULTS AND CONCLUSION: (1) The range of motion of knee in the 3D printing group was larger than that in the conventional group, but had no significant difference at 2 weeks postoperatively (P=0.744). (2) There was no significant difference in the Hospital for Special Surgery scores between two groups at 2 weeks postoperatively (P= 0.532). (3) The postoperative lower limb mechanical alignment showed no significant difference between two groups (t=0.218, P=0.632).(4) The operation time in the 3D printing group was significantly shorter than that in the conventional group (P=0.000). (5) The blood loss in the 3D printing group was significantly less than that in the conventional group (P=0.000). (6) Our findings indicate that 3D printing technology-aided TKA exhibits similar results to the conventional TKA in the Hospital for Special Surgery scores, range of motion, and lower limb mechanical alignment, but it shortens the operation time,reduces the blood loss, and achieves precise osteotomy, which is available for the elderly with poor basic condition, and weak tolerance of surgery.

Honeybee pupae were collected from Jilin apiaries and RNA was extracted for use as a tefnplate for amplification. Based on the complete genome sequences of black queen cell virus (BQCV) published on GenBank, we designed 10 pairs of primers to amplify genes by reverse transcription-polymerase chain reaction (RT-PCR). Using this approach, we have obtained the first complete genome sequence of a BQCV isolate in China. The genome of the isolated strain, named BQCV-JL1, is composed of 8358 nucleotides and shares between 86% and 93% homology with the complete genome sequences of the other six BQCV strains published on GenBank. ORF 1 of BQCV-JL1 is positioned between nucleot ides (nt) 546 and 4676 (4131 nt), while ORF 2 is located between nt 5750 and 8203. Between the two ORFs of BQCV-JL1 there is a short ORF, called ORF 3, between nt 4891 and 5433 (543 nt). The first functional gene ex- pression domain of the BQCV-JL1 strain is positioned between nt 546 and 5 429, encompassing both ORF 1 and ORF 3. There is an internal ribosome entry site (IRES) located before ORF 2, the last three bases of which are CCU (nt 5642-5644). These bases act as an initiation.codon facilitating the translation of ORF 2. The second functional gene expression domain of the BQCV-JL1 strain is located between nt positions 5642 and 8203. The BQCV-JL1 strain was found to share high sequence identity (93%) with the Hungary 10 genotype at the whole-genome level and analysis of the nucleotide and amino acid sequences revealed that the BQCV-JL1 strain also shows close genetic relationships with the South Korea strain, suggesting that both the BQCV-JL1 and South Korea strains may have migrated from European countries. BQCV-JL1 strain was different from the other 6 strains in dividing the nucleotides positions of QRF, which vqs because of the gene mutation.
Amino Acid Sequence ; Animals ; Bees ; China ; Genome, Viral ; Molecular Sequence Data ; Open Reading Frames ; Phylogeny ; RNA Viruses ; classification ; genetics ; isolation & purification ; Viral Proteins ; genetics

Objective Background Pulmonary endarterectomy (PEA) is a safe and effective surgical treatment for chronic thromboembolic pulmonary hypertension. University of California at San Diego Medical Center is widely recognized as the world's leading referral center for PEA surgery with extensive surgical experience, which has surgically treated about 2400 patients till 2009, which account for more than 50% of the total cases in the world. Methods During visiting in UCSD, 32 pulmonary endarterectomy operations were performed by Prof. Stuart W Jamieson and Mechel M Madani. In these patients, 17 were males (53%), the average age was (47.56 ± 16.04 ) years, 47% with prior history of pulmonary embolism and/or deep vein thrombosis. Obvious pulmonary hypertension and hypoxemia were observed in all patients. Bilateral pulmonary endarterectomy was performed under cardiopulmonary bypass with profound hypothermic circulatory arrest. Results According to the classification of surgical specimens, 21.8% are type Ⅰ , 28.1% are type Ⅱ and 37.5% are type Ⅲ. The average time of cardiopulmonary bypass (CPB) was (236.32 ± 37.27) mins. The aortic cross clamp time was ( 111.69 ± 28.14) mins. The circulatory arrest time was (38.00±13.58 ) mins [ right side (21.39 ± 9.57 ) mins and left side ( 16.61 ± 6.83) mins]. Postoperatively the average mechanical ventilation time was ( 66.23 ± 99.24) hours, and the ICU stay was (4.62 ± 4.50 ) days.There was no postoperative moorality. All cases had significant decrease in systolic pulmonary artery pressure [(81.03 ± 16.92)mm Hg vs. (51.20±12.16) mm Hg] and pulmonary vascular resistance [(88.91 ±42.32) kPa · s · L-1 vs. (34.38 ±15.68 ) kPa · s · L-1 ], great improvement in cardiac output [(3.65 ±1.08 ) L/min vs. ( 5.85 ± 1.21 ) L/min ] and central venous pressure [(13.07 ± 2.11) cmH2O vs. ( 9.86 ± 3.02 ) cmH2O] postoperatively compared to preoperative data. Shortterm follow-up showed that the cardiac function of all cases returned to NYHA class Ⅰ or Ⅱ, with great improvement in CTPA.Conclusion PEA is definitive treatment of chronic thromboembolic pulmonary hypertension. According the successful experience of UCSD PEA team, first and foremost pulmonary endarterectomy is a bilateral procedure, because chronic thromboembolic pulmonary hypertension is mostly a bilateral disease. Second, cardiopulmonary bypass with the aid of deep hypothermia and circulatory arrest are essential to operator in recognizing the true endarterectomy plane of the media and in following the specimen to its feathered tail end in each branch, using these approaches can effectively reduce pulmonary hypertension and provide good hemodynamic and symptomatic results. Third, PEA remains an uncommon procedure in China. Only a few centers can perform high qualified PEA surgery. In most of inexperienced centers, type Ⅰ and type Ⅱ disease should be choose to operation.Patients with sPAP ≥100 mm Hg, PVR ≥ 100 kPa · s · L-1 or type Ⅲ disease will face more dangers peri-operation.