Marine medicine is an important part of Chinese medicine and a growth point of the development of marine economy. Current researches of marine medicine had ignored accumulated experience in clinical medicine for thousands of years of development of marine medicine and the guidance of traditional Chinese medicine (TCM) theory. In this article, we proposed a research mode of Mining experience-Clinical validation-Targets screening-Component screening-Component compatibility-Mechanism study under the guidance of the hypothesis of Nature-Effect-Material Trialism to study marine medicine. Kun Hai Jiang Xin (KHJX) Decoction is a marine formula drawn from the experience of ancient physicians by data mining techniques. Clinical study found that KHJX Decoction can significantly improve the overall clinical efficacy of chronic bronchitis with good clinical safety. Under the guidance of TCM herbal theory, object presented here is based on the work of KHJX Decoctionmining experience - clinical validation. The molecular docking method and receptor-ligand binding assay were applied to screen effective components from herbs of KHJX Decoction and the compatibility plan of effective components under the guidance of the principle of cold and heat effects compatibility. This study is of great significance to clarify the material basis and molecular mechanism of KHJX Decoction in the treatment of chronic bronchitis. It provides a new research mode for marine drug research.

Although the origin of Chinese medicine was well recorded inHuang-Di Nei-Jing, different opinions, however, now existed in the authenticity of discussions on five cardinal directions in theYi-Fa Fang-YiLun. From the perspective of regional systems, this paper began with the local culture of Chinese northern nomadic people, analyzed their fire using custom, moxibustion therapy, ice and snow culture and Shamanistic witchcraft. The purpose was to reveal that moxibustion therapy originated from Shamanistic witchcraft and medical practice of the northern nomadic people.

0.05) in results. The positive rate was 26.99% for these 3 kits, and any two of them could achieve a positive rate of 53.95%. The total positive rate was 80.95%, which was significantly different as compared with non-tuberculosis group(P

Calcium content and calcium-calmodulin complex were determined in endothelial cells before and after they were treated with endotoxin.It was found that endotoxin can promote the disintegration of calcium-calmodulin complex and greatly increase the cacium content in the endothelial cells.

Twenty-nine medical records of acupuncture and moxibustion at the end of volume in Zhenjiu Dacheng are precious clinical examples. The characteristics of selecting acupoints are less and ingenious with precious matching on the basis of syndrome differentiation and examining pathogenesis detailedly, according to meridians with diseases locations recognized clearly, keeping adopting acupoints on regular meridians combined with extra points and making good use of specific points, and attaching importance to acquired essence with protecting spleen and stomach. Valuable experience is provided for physicians of later generations to treat diseases.
Acupuncture Points ; Acupuncture Therapy ; history ; Books ; history ; China ; History, Medieval ; Humans

Objective To investigate the dynamic changes of Trk(tropomyosin receptor kinase) and Akt(protein kinase B), and to explore the neuroprotective role and its mechanism of β-NGF (β-nerve growth factor) after focal cerebral ischemia. Methods A focal cerebral ischemia rat model was made and β-NGF was injected intra abdominally. The phosphorylation of Trk and Akt were tested by immunocytochemistry and Western blot assay. Results Trk receptor was increased obviously in the penumbra area after 8 h of infarction. The level of phosphorylated Trk(p-Trk) was increased after 2 h of infarction, while the level of phosphorylated Akt(p-Akt) had a decrease and then recovered gradually. The level of p Akt in cerebral ischemia group was decreased by 76.5% compared with that in control group after 8 h of infarction(P<0. 01). After the injection of β-NGF, the level of p-Trk was increased by 74.4% after 12 h of infarction(P<0.01), while the level of p-Akt was recovered significantly after 8 h of infarction, and had no statistical difference compared with the control group after 24 h of infarction (P>0.05). Conclusions Ischemia induces the activation and increased expression of Trk receptor, andβ-NGF may play a protective role in cerebral ischemia by increasing the phosphorylation of Trk and regulating the phosphorylation of Akt.

Objective To investigate the effect of hand twelve Jing-Well points bloodletting on blood-brain barrier (BBB) permeability in the early stage of focal cerebral ischemic injury in rats with permanent middle cerebral artery occlusion (pMCAO). Method Male Wister rats were randomized into sham operation, model and bloodletting groups. A model of middle cerebral artery occlusion was made by Longa's method. 2%Evans blue (EB) was injected into the caudal vein at three time points:5, 24 and 72 hrs after model making. EB exudation amount was assessed by measurement of EB absorbance in brain tissue to observe BBB permeability. Result Cerebral EB exudation amount was significantly higher in the model group of rats than in the sham operation group (P<0.01) and significantly lower in the bloodletting group than in the model group (P<0.01). Conclusion Hand twelve Jing-Well points bloodletting can reduce blood-brain barrier permeability in pMCAO rats. Its effect is highly significant at 24 and 72 hrs after ischemia.

Objective To evaluate transurethral endosco pic management of 15 cases of ureterocele encountered between 1990 and 2000. Methods All the medical documents were reviewed and analyse d. Results The diagnosis of all the cases was establishe d on IVU,ultrasonography and endoscopy.Transurethral incision of ureterocele was undertaken for 6 cases,and transurethral unroofing for 9.All the patients have been followed up for 6～36 months with the hydronephrosis abated.No vesicoureter al reflux has been noted. Conclusions Transurethral inte rvention of ureterocele is simple and effective rendering minimal trauma to the patients.Transurethral unroofing is an effective means to treat ureterocele.

Objective To make the surgical therapy of urethral stricture and occlusion more effective. Methods 154 patients treated in our hospital were included in this retrospective study,of these cases,40 cases were in anterior urethra and 114 in posterior part.Based on etiology,106 cases were from trauma,15 from infection and 33 from idiopathic causes.Open surgery was performed on 34 cases and endoscopic therapy on 120 cases. Results All of the anterior urethral cases were treated successfully,among them,18 cases were with open surgery and 22 with endoscopic surgery.Normal urination was achieved on the follow-up from 6 months to 12 months.Within posterior urethral stricture and occlusion situation,open surgery was in 16 cases with 10 cases of normal urination and endoscopic surgery in 98 cases with 92 cases of normal urination.6 cases with open surgery and 5 cases with endoscopy resulted in postoperative dysuria or anuria.Urethral stricture and occlusion were shown in all these unsuccessful operation according to our urethrogram. Conclusions Good result can be achieved by both open and endoscopic surgery in anterior urethral stricture.In case of posterior urethral condition,endoscopic procedure is the best choice.

Objective To evaluate whether P53 gene mutation was related with the pathogenesis of ovarian malignant epithelial tumors or not.Methods P53 gene mutation in exons 5-8 was detected by polymerase chain reaction product-single strand conformation polymorphism analysis (PCR-SSCP).Results The mutation in benign tumors: 3(10%) cases, but in malignant epithelial tumor:19(38%) cases(P0 05).Conclusions P53 gene mutation in exons 5-8 was probably related with the pathogenesis of ovarian malignant epithelial tumors, but was not related with histological grade.