Objective To observe the clinical effect of patellar anaplasty and traditional knee arthroplasty in the treatment of osteoarthri-tis. Methods According to the operation method,50 patients with osteoarthritis were divided into patellar anaplasty group and knee arthro-plasty group,25 cases each. After surgery,all patients were followed up. Results The differences of the operation time,intraoperative blood loss,HSS score before and after operation,the WOMAC scores and radiographic evaluation between two groups were not statistically significant (P>0. 05). The complication rate of patellar anaplasty group was lower than those of knee arthroplasty group significantly,and the difference between two groups was statistically significant(P<0. 05). Conclusion Patellar anaplasty and traditional knee arthroplasty can improve the patient’s knee function effectively,and all the curative effect are reliable,but the complication rate of patellar anaplasty is lower,which is worthy of further promotion and research.

0.05). Radiographic analysis showed quite good results in 77 cases. Complications included delayed union in six cases, severe stiffness in one and neuropraxia in two. Conclusions Open reduction and internal fixation of intracondylar humeral fractures is a safe and effective technique. Meanwhile, triceps-split approach and olecranon osteotomy can obtain same clinical outcome for intracondylar humeral fractures.

Objective To observe the findings of 1H magnetic resonance spectroscopy ( 1H MRS) and explore its value for diagnosis of radiation induced-temporal lobe necrosis in nasopharyngeal carcinoma (NPC). Methods Thirteen patients with definite clinical diagnosis of radiation induced temporal lobe necrosis in NPC underwent 1H MRS. All 1H MRS examinations were performed on a Singa Horizont 1.5 T whole body MR system with point resolved spectroscopy (PRESS) and chemical-shift selective saturation (CHESS) (TR=2000?ms,TE=144 ?ms). The voxel (2?cm ? 2?cm ? 2?cm) was positioned in both of the lesion and the contralateral side of the temporal lobe as the control (except for bilateral lesions). 2THZ]Results The interval between radiation and the examination ranged from 32 to 172 months ( median, 42 months). Eighteen lesions were observed in 13 patients (5 patients with bilateral lesions). 1H MRS was performed on 17 lesions and 8 control temporal lobes. The mean values of NAA/ Cr in the lesions and the control temporal lobes were 1.33?0.28、1.78?0.34 respectively(t=3.22, P=0.005). Three lesions showed NAA、Cho and Cr peak absent (2 lesions with Lip peak ). Among 8 patients with the control temporal lobes 1H MRS examined, Cho/Cr decreased in 6 patients (75%). Four lesions showed Lip , Lac and MI, respectively. Conclusions The radiation-induced temporal lobe necrosis shows abnormal features on 1H MRS. Significant decrease of NAA/ Cr as well as Cho/Cr are observed in most patients. 1H MRS may play a role in the diagnosis of radiation induced temporal lobe necrosis.

Objective To investigate clinical results of shoulder hemiarthroplasty in treating comminuted proximal humeral fractures. Methods Shoulder hemiarthroplasty was performed from May 1997 to June 2004 in 28 cases with comminuted proximal humeral fractures who were followed up for mean 2.1 years. American Shoulder & Elbow Surgeon (ASES) score was adopted for evaluating pain after operation, range of active movement and function of daily work. Results Mean ASES score was 87.9(67-96 scores), with mean forward flexion of 132?, external rotation of 40?, mean VAS score of 2.6 and internal rotation at T_ 10 level. Strength and function of 23 cases reached 80% of the normal side six months postoperatively and 90% of the normal side one year postoperatively. Of all cases, 82% were satisfied with the treatment. Conclusion Only reconstruction of normal humeral length, tuberosity fixation and persevering rehabilitation can contribute to a successful result of hemiarthroplasty for the comminuted proximal humeral fractures.

Interest teaching method aims to inspire students' initiative and inquiry in learning.In the teaching process,we used some teaching methods including class lead-in,relevant extracurricular books and websites,scene representation,lectures on special content and arrangements of the review writing.It is important to maintain students' motivate and interest in learning human parasitology,therefore,we should no longer make students passively accept but the initiatively make inquiry learning.

Objective To investigate the protective effect of cryptotanshinone (CTS) and donepezil(DON) on amyloid-βprotein (Aβ)-induced apoptosis in SH-SY5Y cells .Methods SH-SY5Y cells were cultured in vitro for establishing the Alzheimer disease (AD) model .The cell viability was detected by the MTT assay .The apoptosis rate was measured by Hoechst 33342 and the expres-sion of Bcl-2 and Bax was detected by Western blot .Results CTS ,DON and their combination could obviously alleviate Aβ-caused injury of SH-SY5Y cells ,increase the cell survival rate ,remarkably up-regulate the expression of Bcl-2 protein ,decrease the expres-sion of Bax protein and inhibit the apoptosis .The effect of the CTS and DON combination for inhibiting apoptosis was significantly stronger than that of the single use of CTS and DON ,the difference had statistical significance(P<0 .05) .Conclusion The combi-nation of CTS and DON has the synergistic protective effect on Aβ-caused injury in SH-SY5Y cells ,its mechanisms may be related with the cooperation regulation of the expression of apoptosis related gene Bcl-2 protein family .

In order to stimulate students to interest in study,broaden their horizons,and strengthen their scientific literacy,the writers opeed the elective course of medical microbiology---《plague and the movie》.During this teaching process the writers explored the setting of the elective course,and summarized the experience and the existing problems of the process and prospect the teaching work of the future.

Thirty two upper limbs of 16 adult fresh cadavers were studied.Suspension ofbarium sulfate in red latex were injected prior to medial arm flap dissection.Thearm flaps were observed and measured under the magnifying spectacles(3X),and X-ray photographs were taken.The main results obtained are summarized as follows:1.In 30 specimens,there are 386 branches arising from the brachial artery——pure cutaneous branches(67,17.36?1.93%),pure muscular and myocutaneous branches(186,48.182?2.54%)and mixed branches(133,34.46?2.42%).2.The brachial profundal artery arises from the brachial artery about 2 to 3cm.below the lower margin of pectoralis major.The caliber of the artery is 2.22?0.14mm.It supplies the triceps brachii and the skin of medial and dorsal surfaceof the upper part of arm.3.The superior ulnar collateral artery arises from the brachial artery about 6cm,below the lower margin of the pectoralis major.The caliber of the artery is1.71?0.08mm.It supplies the long and medial head of Triceps Brachii and the skinof middle and lower part of arm.It is a main artery of medial arm flap.4.The inferior ulnar collateral artery arises from the brachial artery about 18cm,below the lower margin of pectoralis major.The caliber of the artery is 1.50?0.07mm.5.The myocutaneous artery of biceps brachii arises from the brachial arteryabout 8cm,below the lower margin of pectoralis major.The caliber of the artery is1.63?0.13mm.It gives off a larger cutaneous artery.6.An abundance of anastomoses is formed by these cutaneous arteries and arich network of arteries may be seen.The caliber of some of the cutaneous arteries isabove 1mm.7.The venous drainage consisted of venae comitants.The medial arm flap is generally thin,soft and non-hair-bearing,the caliberand length of the pedicles of the arteries are large enough for microsurgical vasoanas-tomosis.It is a suitable donor site for free transfer of skin flap and the optimaldimension is 8?20cm.

Objective To investigate the function of SD rats Schwann cells on the growth and differentiation of adipose-derived stem cells(ADSCs) when they are co-cultured in Transwell culture system. Methods The ADSCs harvested from SD rats were divided into 3 groups. Group A: ADSCs and Schwann cells were cultured in Transwell indirect co-culture system. Group B: ADSCs were induced by β-BME and BHA. Group C: a contrast group. The morphology of ADSCs was observed and immunohistology was pedormed. Results ADSCs of group A and group B were partly differentiated to the cells which had long neurite,and had a positive result in the NF staining, but negative in the GFAP staining. Group A shows no great difference in neurite length compared with group B(P < 0.05), and no great difference in cell quantity compared with group C (P < 0.05). Conclusion Schwann cells of SD rat have a growth supportive and neuronal differentiation inducing effect on ADSCs.

ObjectiveTo evaluate arterial stiffness and its influencing factors in the elderly patients with hypertension and (or) type 2 diabetes mellitus.MethodsTotally 320 elderly patients were divided into four groups: control,diabetes mellitus,hypertension,coexisting hypertension and diabetes mellitus.Arterial stiffness in ankle and brachial artery was determined by brachial-ankle pulse wave velocity (baPWV).Other factors affecting baPWV were also collected and measured,including age,body mass index (BMI),sex,smoking,blood pressure,pulse pressure,mean arterial pressure,serum fasting blood glucose,lipid profile,glycosylated hemoglobin and hypersensitive C-reactive protein (hsCRP).ResultsbaPWV in the four groups were ( 1667.2 ± 279.3 )cm/s,(2158.6 ±386.9)cm/s,(1881.2±383.8) cm/s and (2165.9± 479.9)cm/s,respectively.The baPWV value was higher in groups of hypertension,diabetes mellitus,and the coexisting diseases than in controls (F=8.473,P＜0.05).Diabctic patients had a higher baPWV levels than those without diabetes.There was no difference in baPWV levels between diabetic patients with or without hypertension.Pulse pressure and hsCRP were higher in coexisting diseases group than in control group (P＜0.05).Stepwise backward regression analysis showed that fasting blood glucose,pulse pressure,mean blood pressure,hsCRP and low density lipoprotein cholesterin (LDL-C) were positively related to baPWV P ＜0.05). ConclusionsHypertension and (or) type 2 diabetes mellitus are important influencing factors for higher arterial stiffness. Hyperglycemia might play more important role in the elderly arterial stiffness.Serum fasting blood glucose,pulse pressure,mean blood pressure,hsCRP,LDL-C may be the independent risk factors of arterial stiffness.