Objective To explore the effects of bile from patients with choledocholithiasis on the growth of human cholangiocarcinoma cell line QBC939 and the potential relation between choledocholithiasis and cholangiocarcinoma. MethodsWT5”BZ Choledocholithiasis bile (CB) and normal bile (NB) specimen were used for this study. The proliferative effects of bile were measured by methabenzthiazuron (MTT) assay and cell cycle and apoptosis were analyzed by flow cytometry. KG2Results CB significantly promoted the proliferation of QBC939 cells, QBC939 proliferative index increased significantly after treated with 1% CB for 48 h ( P

Objective To explore the effects of bile in patients undergoing transduodenal sphincteroplasty (TSB) on the growth of human cholangiocarcinoma cell line QBC939 and the potential relation between transduodenal sphincteroplasty and cholangiocarcinoma. Methods TSB and normal bile (NB) sample were used for this study. The proliferative effect of bile was measured by using methabenzthiazuron (MTT) assay, and cell cycle and apoptosis were analyzed by using flow cytometry. Results Compared with NB,TSB significantly promote the proliferation of human cholangiocarcinoma QBC939 cells (P

ObjectiveTo investigate the relationship between the expression of ER,PR,HER-2,Ki-67 and the effective rate of neoadjuvant chemotherapy (NAC) and to study the influence of NAC on expression of ER,PR,HER-2 and Ki-67 in breast cancer.Methods41 patients of breast cancer were performed 2 to 6 cycles of CAF chemotherapy.The expression of ER,PR,HER-2 and Ki-67 was detected by SP immunohistochemical method before and after NAC.ResultsThe effective rate of NAC was 73.17％ (30/41).The positive and negative efficiency rate was 67.86％ (19/28) vs 84.62％ ( 11/13 ) for ER,64.29％ (9/14) vs 77.78％ (21/27 ) for HER-2,70.00％ (14/20) vs 76.19％ ( 16/21 ) for Ki-67.The difference had no statistical significance (P ＞0.05).The positive and negative efficiency rate was 66.67％ (16/24) vs 82.35％ (14/17) for PR.The difference had statistical significance (P ＜ 0.05 ).The expression of ER,PR,HER-2 and Ki-67 before and after NAC changed in 10,8,3 and 9 cases respectively.The difference had no statistical significance (P ＞ 0.05).ConclusionsNAC has no influence on the expression of ER,PR,HER-2 and Ki-67.Patients with PR negative are more sensitive to NAC.The expression state of PR can be used to predict the curative effect of NAC.The expression state of ER,HER-2 and Ki-67 has no significant correlation with NAC.

Objective To observe the effect of octreotide on human pancreatic cancer cells (PC-3) apoptosis after PC-3 transfected with somatostatin receptor type 2 (SST 2) gene. Methods SST 2 was transfected into PC-3 by liposome,the result of transfection was detected by immunohistochemistry. The apoptosis of PC-3 induced by using different dosage of octreotide were detected by MTT assay and flow cytometry. Results The effects of killing PC-3 by different dosage (0.2,0.4 and 0.8?g/ml ) of octreotide in transfected groups were significantly stronger than those in non-transfected groups(P

Antineoplastic Agents ; pharmacology ; Bile ; physiology ; Bile Duct Neoplasms ; metabolism ; pathology ; Bile Ducts, Intrahepatic ; Celecoxib ; Cell Division ; drug effects ; Cell Line, Tumor ; Cholangiocarcinoma ; metabolism ; pathology ; Cyclooxygenase 2 ; Dinoprostone ; metabolism ; Humans ; Isoenzymes ; biosynthesis ; genetics ; Membrane Proteins ; Prostaglandin-Endoperoxide Synthases ; biosynthesis ; genetics ; Pyrazoles ; RNA, Messenger ; genetics ; Sphincterotomy, Transduodenal ; adverse effects ; Sulfonamides ; pharmacology ; Up-Regulation