Objective To explore the correlations of lung resistance protein (LRP) and glutathione S transferase π (GST-π) to chemotherapy resistance and prognosis of epithelial ovarian cancer.Methods The expressions of LRP and GST-π in epithelial ovarian cancer were examined with immunohistochemistry.Correlations of LRP and GST-π to chemotherapy efficacy and survival time after operation were analyzed.Results The short-term efficacy rates of ovarian cancer were lower in patients with positive expressions of LRP and GST-π than those with negative expressions [61.2％,61.7％ vs 94.1％,89.5％,x2 =6.47,4.94,P =0.011,P =0.026].The positive rates of LRP and GST-π were significant higher in patients with chemotherapy resistance than in those sensitive to chemotherapy [91.3％,87.0％ vs 65.1％,62.8％,P ＜ 0.05].Log-rank test showed that patients with positive LRP and GST-π had shorter survival time than those negative,and patients with both positive LRP and GST-π had shorter survival time than those both negative (P ＜ 0.05).Conclusions The expressions of LRP and GST-π in epithelial ovarian cancer could be used to predict chemotherapy resistance and prognosis of patients.

Objective: The Thinprep liquid-based cytology test(TCT) is a new technique applied to cytopathologic diagnosis in recent years.The purpose of this study was to analyze the diagnostic coincidence between lung puncture liquid-based cytology and histopathology.Methods: A total of 408 tissue specimens obtained by CT-guided percutaneous translung biopsy were observed under the optical microscope after fixation,embedding,sectioning and staining,and the cells in the washing fluid for puncture needles were detected by TCT.Then the diagnostic coincidence was analyzed between cytological and pathological biopsies.Results: Of the 408 cases detected by liquid-based cytology,148(36.3%) were diagnosed as cancer,32(7.8%) as suspected cancer,38(9.3%) as heterocyst and 190(44.9%) were negative,as compared with 183(44.9%),21(5.1%),22(5.2%) and 182(44.6%) by histopathology.The results showed a correlation between the two methods(P

Objective: To investigate the advantage of ThinPrep cytological test in tumor diagnosis by fine needle aspiration cytology. Methods: Using fine needle aspiration for smears,some of smears were made in conventional smears,the remains aspirating needles were washed with a transport washing solution.ThinPrep slides were made using the ThinPrep Processor. Results: In 84 cases FNA samples,49 cases were breast,29 cases were lymph node and 6 cases were others.Comparing with conventionally prepared specimens,ThinPrep processed samples showed the advantage in sensitivity or specificity.The lack of cellular debris,blood and exudates improve the ability to detect cellular abnormalities and speed up the time required for screening in ThinPrep processed samples.Rapid cell transfer from the sampling device to the transporing fluid containing the fixative ensure preservation of cell details with minimal artifactual changes. Conclusion: The use of Thinprep and by combination of FNAC and immunocytochemistry cytopathological diagnosis can be improved significantly.