Objective To explore the epidemic characteristics,clinical features and treatment outcome of brucellosis in the Dali area of Yunnan province.Methods The clinical data of 43 cases with brucellosis from Janurany 2012 to September 2015 were retrospectively analyzed.Results Among 43 cases,there were 35 males,8 females, 37 farmers,5 veterinary,and 1 teacher.42 patients had a clear history of contact with cattle and sheep,1 case of no clear history of exposure to cattle and sheep,mainly fever,accompanied by chills,headache,joint pain,low back pain, weight loss,hepatosplenomegaly etc.Laboratory routine examination showed no specificity,SAT was detected in 30 cases,positive rate was 100.0%,blood culture in 28 cases,23 cases were positive,the positive rate was 82.1%. 41 cases of adult patients with rifampicin and tetracycline or doxycycline + levofloxacin and cefotaxime drugs combined therapy,treatment for 6 weeks,2 cases of children took rifampicin and SMZ -TMP treatment for 6 weeks, improvement rate was 100%,there was no recurrence and death cases.Conclusion Dali area is popular in brucellosis, the clinical manifestations and the infection way diversification,need the attention of the clinicians.

ObjectiveTo investigate the antioxidant effect of matrine and its clinical effect in the treatment of rats with nonalcoholic steatohepatitis (NASH). MethodsA total of 30 rats were randomly divided into three groups: control group, NASH group, and matrine group, with 10 in each. A high-fat diet was used to establish the rat model of NASH, and matrine was given by gavage for treatment at a dose of 36 mg·kg-１·d-1. The changes in body weight and liver weight were observed in all rats. HE staining was used to observe the histopathological changes of the liver. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the content of reduced glutathione (GSH), superoxide dismutase (SOD), and malondialdehyde (MDA) in liver tissue were measured. A one-way analysis of variance was used for the comparison between multiple groups, and the SNK-q test was used for further comparison between any two groups. ResultsCompared with the NASH group, the matrine group had significant reductions in the serum levels of ALT and AST (ALT: 52.0±3.0 U/L vs 41.8±3.7 U/L, P＜0.001; AST: 233.6±9.4 U/L vs 170.1±1.8 U/L, P＜0.001). The matrine group showed marked improvement in the histopathological changes of the liver compared with the NASH group. Compared with the NASH group, the matrine group had significantly increased content of SOD and GSH in liver tissue (SOD: 17.7±2.0 μg/mg vs 27.0±3.6 μg/mg, P＜0.001; GSH: 16.5±1.6 U/mg vs 28.5±2.1 U/mg, P＜0.001) and significantly reduced content of MDA (22.9±1.9 nmol/mg vs 17.8±1.8 nmol/mg, P＜0.001). ConclusionMatrine has an antioxidant effect and a marked clinical effect in the treatment of rats with NASH.

Objective:To predict the transmembrane structure of transmembrane protein 26 (TMEM26), observe its expression in human retina and mouse retina, and investigate the relationship between it and primary open-angle glaucoma (POAG).Methods:The transmembrane structure of TMEM26 in human and mouse was obtained by inputting its amino acid sequences into the transmembrane protein structure prediction software, MemBrain.The expression and location of TMEM26 in human and mouse retinas were observed through frozen retinal sections stained with anti-TMEM26 antibody, which came from a human donor and five SPF-grade C57BL/6 mice.The possible function of TMEM26 gene and its influence on eyes were inferred on the basis of the specific expression of TMEM26 in retina.The single nucleotide polymorphism mutation of TMEM26 gene was searched in literature related to ocular diseases.The use and care of animals complied with the Regulations on the Management of Experimental Animals.This research protocol was approved by an Ethics Committee of Sichuan Provincial People's Hospital (No.2019-36). Results:Both human and mouse TMEM26 were eight transmembrane proteins with similar eight hydrophobic transmembrane domains, four hydrophilic cytoplasmic domains and five hydrophilic extracellular membrane domains.Small differences in the number of amino acid residues in the domains of TMEM26 were found.In both human and mouse retina, TMEM26 gene was only specifically expressed in the outer plexiform layer (OPL)and inner plexiform layer (IPL). TMEM26 was weakly associated with POAG in a published data. Conclusions:TMEM26 is a multi-pass transmembrane protein, mainly expressed in IPL and OPL of the retina. TMEM26 gene is weakly related to POAG.