Objective:To explore the prevalence of posttraumatic stress symptoms among preschool children and its related factors after the Ludian earthquake.Methods:A total of 62 children's guardians were visited.They were interviewed with the Posttraumatic Stress Disorder Semi-Structured Interview for Infants and the Young Children and the Child Behavior Checklist-Posttraumatic Stress Disorder scale (CBCL-PTSD) consisted of 15 items from the CBCL.All of the participantsresided in Longtoushan settlement and Longtoushan town (the epicenter).They were randomly visited from west to east in that region.Results:The rate of scored 9 (or more) on the CBCL-PTSD among children was 51.9％ (27/52) in 1-2 months after the earthquake.However,based on the Semi-Structured Interview,the rate of the children's posttraumatic stress symptoms was 26.9 ％ (14/52).The numbers of posttraumatic stress symptoms,intrusion symptoms,avoidance symptoms,hyperarousal symptoms,the new symptoms and the CBCL-PTSD scores were positively correlated (0.30-0.73,P ＜0.05),except the relation between intrusion symptoms and hyperarousal symptoms.The rate of intrusion symptoms was significant higher among those who experienced earthquake than those who didn't (96.2％ vs.60.0％,P ＜0.01).And the rate of intrusion symptoms was also higher among those who was injured during the earthquake than those who wasn't (100％ vs.84.6％,P ＜ 0.05).And the other symptoms were not difference in children under different conditions (P ＞ 0.005).Conclusion:The preschool children who experienced earthquake or were injured suffer more posttraumatic stress symptoms.

Objective To conduct a preliminary examination of the factor structure and the reliability and validity of a revised Chinese version of the State-Trait Anxiety Inventory-Form Y (STAI-Y) by using the large sample of migrant children.Methods Perform Exploratory Factor Analysis with half of the data (n =5477) from a large-scale questionnaire survey of students in Grades 4-9 at 58 schools for migrants in Beijing,then conduct Confirmatory Factor Analysis with the other half (n =5476).Results The results of state anxiety and trait anxiety both showed two factors.However,the indicators of trait anxiety were not ideal as seen from the following:the total explained variance was 39.22％,the relationship between the factors and items was not in accordance with the original English version,and the correlation between the two factors was unstable in the different samples (r =-0.17,P ＜ 0.001 ; r =-0.06,P ＜ 0.001).In addition,the factor loading of items 24 ( I wish I could be as happy as others) was low.Conclusion The state anxiety portion of this revised Chinese version of the STAI-Y is structured by the factors state anxiety present and state anxiety absent,and with good reliability and validity as well.Nevertheless,the trait anxiety portion of this revised Chinese version of the STAI-Y is structured by the factors trait anxiety present and trait anxiety absent,it shows unstable reliability and validity.

Objective:To examine the relationships between acute stress disorder (ASD) symptoms,posttraumatic stress disorder (PTSD) symptoms and depressive symptoms in children survivors following the Lushan earthquake in China.Methods:One hundred ninety-seven children aged 1 to 14years (93 male and 104 female) were followed up at two and six weeks after Lushan earthquake.The ASDS,UCLA PTSD Reaction Index and DASS were used to measure the ASD,PTSD and depressive symptoms.Results:The average scores of ASDS and the depression subscale of DASS at two weeks after earthquake were(36.5 ± 10.9) and (3.3 ± 2.8),respectively.The average scores of UCLA PTSD Reaction Index and the depression subscale of DASS-21 at six weeks after earthquake were (18.5 ± 12.6) and (3.3 ± 3.5),respectively.The rates of ASD and PTSD were 28.6％ and 8.2％,respectively.The cross-lagged structural equation analysis with latent variables indicated that the ASD symptoms at two weeks after earthquake positively predicted PTSD symptoms (β =0.59,p ＜ 0.001) and depressive symptoms (β =0.29,p ＜0.001) at six weeks after earthquake.But there was no significant association between depressive symptoms at two weeks and PTSD symptoms at six weeks after earthquake.Conclusion:ASD symptoms in the early time postearthquake could predict subsequent PTSD and depressive symptoms,while depressive symptoms tend to remain stable within six weeks after earthquake.

Objective : To investigate the mediating effect of negative emotions on visual impairment and resilience among middle-aged and elderly populations, so as to provide insights into improving psychological health among middle-aged and elderly populations. Methods: Demographic information, visual impairment, negative emotions and resilience in the residents at ages of 45 years and older were collected through the database of the Yushu Physical and Mental Health Survey carried out by Institute of Psychology of Chinese Academy of Sciences. Structural equation model was used to analyze the effects of visual impairment and resilience and the mediating role of negative emotions. Results: Totally 2 997 middle-aged and elderly populations were enrolled, with the median age of 50.00 (interquartile range, 47.00) years. There were 1 320 males (44.04%) and 1 677 females (55.96%), 1 627 illiterate persons (54.29%), and 2 780 married persons (92.76%). The scores of visual impairment, negative emotions and resilience were (6.79±2.59), (6.35±3.05) and (33.38±10.41) points, respectively. The results of structural equation model analysis showed that after adjusting for age, gender, educational level and marital status, visual impairment had a direct negative positive effect on resilience, with a direct effect value of -0.157 (95%CI: -0.177 to -0.137), and visual impairment could also negatively affect residence through negative emotions, with a mediating effect value of -0.112 (95%CI: -0.126 to -0.104), and the mediating effect contributed 41.64% to the total effect. Conclusion visual impairment could reduce residence directly or indirectly through the mediating effect of negative emotions among middle-aged and elderly populations.

Objective To improve mental health of children in the rural area by intervention with moviewatching.Methods The participants were 438 students from Anhui province,including 273 students in grade 5 from two primary schools and 165 students in grade 2 from two middle schools.Subjects were randomly divided into two groups:an intervention group with movie-watching (n=188)and a control group(n=250).Results The inter-group difference between the pre-measurement and the post-measurement on depression and anxiety of the intervention groups and the control groups in grade 5 from two primary schools was not significant,while the intergroup difference between the pre-measurement and the post-measurement on value8(the intervention group (2669.52±2216.43),the control group(316.57±1410.83)),and the inter-group difference was significant(t_(values)=10.721,P=0.000).The inter-group difference between the pre-measurement and the post-depression, anxiety and values of the interntion groups and the control groups in grade 2from two middle schools was significant(t _(depression)=2.046,P=0.043;t _(state amxoetu)=3.507,P=0.001;t _(trait anxiety)=3.392,P=0.001;t _(values)=5.022,P=0.000)respectively).The intervention effect on mood of depression,anxiety and the values of intervention groups in grade 5 from two primary schools was not significant,while significant on values(F=91.62,P=0.000);while that of intervention groups in grade 2 from two =0.000 respectively).Conclusion Movie-watching intervention wag an effective way to improve middle school students' mental health and to develop correct values.Movie-watching should be an alternative way to improve met-al health of children in rural area for its operability.

Objective To test the reliability and validity of the short version of depression-anxiety-stress scale (DASS-21) for students in the disaster region.Methods DASS-21 and the Chinese version of UCLA PTSD Reaction Index for DSM-IV,Revision I were used to evaluate psychological health among 876 students from grades 5 to 9 after Ya' an earthquake.Results (1) Each of the items had a good distinction degree,with CR value ranging from 9.268 to 22.438 (P＜ 0.01),and the correlation coefficients ranged from 0.306 to 0.742.(2)The Cronbach' s alpha coefficients ranged from 0.781 to 0.911.(3) The correlation coefficients between total score and each dimension ranged from 0.714 to 0.914,and the correlation coefficient between PTSD and total DASS score,depression anxiety and stress score was 0.626-0.774.(4)Through the item analysis and explore factor analysis,the revised scale contained 21 items and 3 subscales which could explain 47.813％ of the total variance(KMO =0.937,x2 =3126.85,df=210,P＜0.01),and the results of confirmatory factor analysis supported the three-factor model (x2/df=4.180,P＜0.01,PCFI =0.728,CFI =0.904,IFI =0.905,TLI =0.881,and RMSEA =0.060),and the load of each item was between 0.339 to 0.715.Conclusion The short version of DASS-21 is reliable and valid,and can be used as a tool for post-disaster stress assessment on local students.

Objective To investigate the expression of Epstein-Barr virus(EBV)-latent membrane protein 1 (LMP1) in chronic atrophic gastritis (CAG) with intestinal metaplasia and discuss its effect on gastric cancer.Methods Immunohistochemistry was used to examine the expression of EBV-LMP1 in 45 cases of chronic superficial gastritis(CSG),63 cases of CAG with intestinal metaplasia and 36 cases of gastric cancer.Results There was no expression of EBV-LMP1 in CSG and gastric cancer,while the positive rate of EBV-LMP1 in CAG with intestinal metaplasia was 36.5％ (23/63) and EBV-LMP1 was mainly stained in the cell nucleus.The expression of EBV-LMP1 in CAG with intestinal metaplasia was significantly higher than that in CSG and gastric cancer,and there was significant difference (P =0.000).Conclusions EBV-LMP1 is expressed in CAG with intestinal metaplasia.The expression of EBV-LMP1 is significantly higher than that in CSG and gastric cancer indicating that EBV infection in gastric carcinogenesis may play an important role in the early stages.

Objective:To investigate the expression and clinical relevance of micro RNA (miR)-324-5p in pancreatic cancer tissues, and to explore the effects and potential mechanisms of miR-324-5p on the proliferation and migration of pancreatic cancer cells.Methods:Real-time quantitative PCR was used to detect the expression of miR-324-5p in 34 pairs of pancreatic cancer and adjacent normal tissues resected at Peking University First Hospital from October 2018 to September 2019. The correlations between miR-324-5p expression and clinicopathological characteristics and prognosis of pancreatic cancer were analyzed using data from the Cancer Genome Atlas (TCGA) database. Real-time PCR was used to detect the expression of miR-324-5p in pancreatic cancer cell lines, and PANC-1 cell was used for functional study by overexpressing miR-324-5p via mimic transfection. CCK8 assay was used to evaluate cell proliferation. Both transwell and scratch wound healing assay were used to assess the cancer cell migration ability. Related proteins were detected by Western blot. The potential downstream target genes of miR-324-5p were selected using data from miRNA target genes predicted webs, in combination with functional analysis and their expressional correlation with miR-324-5p.Results:Data from TCGA database showed that the expression of miR-324-5p in tumor tissues was significantly lower than that in normal pancreatic tissues. And low level of miR-324-5p in pancreatic cancer was correlated with poor prognosis. Analysis of 34 pairs pancreatic cancer and adjacent normal tissues showed that miR-324-5p expression in tumor tissues (11.7±2.0) was significantly lower than that in adjacent normal tissues (70.9±14.4), and the pancreatic cancer patients who had the nerve invasion cancer showed low level of miR-324-5p (82.1%, 23/28) was significantly higher than that patients with high level of miR-324-5p (33.3%, 2/6). The expression of miR-324-5p in human pancreatic cancer cell line was also significantly lower than that in normal pancreatic ductal cells. CCK-8 assay showed that the proliferation ability of PANC-1 cell was significantly decreased when miR-324-5p was overexpressed. Transwell and wound healing assays showed that the capabilities of vertical migration and the horizontal movement were significantly inhibited in PANC-1 cell with miR-324-5p overexpressed [(30.11±5.2) and (174.6±27.0) μm, respectively] than those in control groups [(63.6±4.2) and (458.3±22.3) μm, respectively]. Moreover, Western blots showed a significant overexpression of miR-324-5p inhibited epithelial-mesenchymal transition (EMT). According to the data from miRNA target genes prediction and the functional analysis we found KLF3, MGAT3, PBX1 and ZNRF2 were considered as the potential downstream target genes of miR-324-5p.Conclusions:Our results indicated that miR-324-5p is lowly expressed and acts as the tumor suppressor gene in pancreatic cancer, and low level of miR-324-5p is correlated to a higher rate of nerve invasion and poor prognosis. In human pancreatic cancer cell, miR-324-5p may regulate EMT by directly inhibiting target genes such as KLF3, MGAT3, PBX1, ZNRF2, which in turn suppresses cancer cell proliferation and migration.

Objective To compare the cuffed renal vein technique and the classical techniques in kidney transplantation.Method The classical techniques of mouse renal transplantation required clamping both vena cava and aorta simultaneously and carried out suture anastomoses of the renal artery and vein in a heterotopic position.In our laboratory,we have successfully developed mouse orthotopic kidney transplantation for the first time,using a rapid cuffed renal vein technique for vessel anastomosis,wherein the donor's renal vein was inserted through an intravenous catheter,folded back and tied.During grafting,the cuffed renal vein was directly inserted into the recipient's renal vein without the need for clamping vena cava and suturing renal vein.Result This technique allowed for the exact transplantation of the kidney into the original position,compared to the classical technique,and had significantly shortened the clamping time due to a quicker and more precise anastomosis of renal vein as described.The renal vein anastomoses time was dramatically shortened in cuffed renal vein technique (4 min) as compared with the classical technique (9 min,P＜0.001).This also allowed for a quicker recovery of the lower extremity activity,reduction in myoglobinuria with resultant kidney graft survival of 88.9％.Conclusion The cuffed renal vein technique simplifies microvascular anastomoses and affords important additional benefits.

Objective To establish a dual liver transplantation rat model,which could benefit the future clinical practice.Methods Y type vein derived from the crossover segment of vena cava and two iliac veins in donor and Y type bile duct prosthesis were employed to recanalize portal vein and bile duct from dual liver grafts to recipient liver.The dual right upper lobes with about 45％ ～ 50％ of the recipient liver volume were taken as donor.One was orthotopically implanted at its original position,while the other was rotated 180° sagittally and heterotopically positioned in the left upper quadrant.Survival rate was analyzed to evaluate the function of dual liver grafts.Results A total of 7 rats which underwent dual liver transplantation survived more than 7 days and the survival rate was 58.3％.5 rats died due to abdominal hemorrhage,bile leakage and liver abscess.Conclusion Using Y type vein and bile duct prosthesis,we successfully established a novel rat model of dual right upper liver lobe transplantation.