1.Recognition of protein transduction domain by support vector machine
International Journal of Biomedical Engineering 2010;33(4):205-208
Objective To predict protein transduction domain (PTD) which is a short peptide with the ability to pull diverse molecules across cell membranes.Methods Every peptide segment including PTDs and peptide sequence from SwissProt database was represented by 68 numerical values,which reflected their physicochemical and conformational properties related to the PTD' s membrane penetrating function.Transductive support vector machine (TSVM) and support vector machine(SVM),combined with cluster method,was introduced to predict new PTDs from the peptide segments,which were extracted from SwissProt database.Results TSVM prediction model achieved 94%±4% accuracy and SVM model achieved 94%±5% accuracy.1210 possible PTDs were predicted using the classifiers based on these two models.Conclusion The research provides a guide to find more PTDs in molecular biology experiments and will be helpful in the understanding of the mechanism of PTDs and their function in vivo.
2.An Experimental study on the origin of circulating endothelial cells from the conversion of vascular endothelial cells
Journal of Third Military Medical University 1983;0(03):-
Obsevation on the circulating endothelial cells (CEC) can be used to study the changes of the vascular endothelial cells (VEC) in vivo,which is quite valuable in its clinical application.Neverthiless.the origin of CEC remains not clarified.In this study,the origin of CEC was investigated with cultures of the endothelial cells of human umbilical vein.It was found that CEC is a kind of cells existing in circulating blood and originating from desquamated VEC under physiological conditions.The direct proof of this conversion was provided.And the conversion of VEC to CEC is a process of steady progression.
3.Morphological changes of the lungs after blast,shell-fragment, and blast-fragment combined injuries in dogs
Jianzhao HUANG ; Zhihuan YANG ; Zhengguo WANG
Journal of Third Military Medical University 1984;0(02):-
After the dogs were inflicted with blast injury,high velocity shell fragment injury on one extremity,and blast-fragment combined injury,the morphological changes of the lungs were examined under gross inspection and with optical and transmission electron microscopes,and the lung/body weight index and lung water ratio were determined.It was found that high velocity shell fragment injury on one extremity could intensify the blast injury on the lungs by one grade.
4.Changes of prostacyclin and thromboxane and their significance in blast,shell-fragment and combined blast-fragment injuries in dogs
Jianzhao HUANG ; Zhihuan YANG ; Zhengguo WANG
Journal of Third Military Medical University 1983;0(03):-
The changes of mean pulmonary arterial pressure (PAP) and 6-keto-PGF1a and TXB2 in the plasma and lung tissues of 3 groups of dogs (7 in each group) with blast injury (group Ⅰ),high velocity shell fragment injury on one of the extremities (group Ⅱ),and combined injury of the both (group Ⅲ) were investigated.It was found that PAP and TXB2 in the plasma and lung tissues increased after all the 3 kinds of injuries.The changes of PAP was most marked in group Ⅰ while those of TXB2 was more marked in groups Ⅲ and Ⅲ than in group Ⅰ.6-kcto-PGF1a increased in the plasma and lung tissues after blast and shell fragment injury but decreased in the plasma and showed no changes in the lung tissues after combined injury.The significance of these changes of 6-keto-PGF1a and TXB2 in the 3 kinds of injuryies were discussed.
5.Experimental study on the effects of tumor necosis factor on the cultured human umbilical vein endothelial cells
Yingbo YANG ; Zhengguo WANG ; Peifang ZHU
Journal of Third Military Medical University 1988;0(06):-
The effects of tumor necrosis factor (TNF) on the activity of plasmogen acitva-tor inhibitor (PAD and the morphology of the cells were observed in the original passage of cultured human umbilical vein endothelial cells (HUVEC). It was found that the TNF effect on the PAI was determined by the dosage of TNF, that is, 500U/ml of TNF could significantly activate PAT activity while 2500U/ml markedly inhibit it. Morphdogical observation canfirmed that 2500U/ml of TNF could injure HUVEC to form a kind of cells whose morphology was quite similar to that of circulating endothelial cells originating from peripheral blood.These findings suggest that TNF can stimulate HUVEC to increase the PAI acivity but too large a dosage of TNF could injure the metabolic function and structure of HUVEC.
6.Effects of nociceptin/orphanin FQ on cross-organ sensitization in rat model with visceral hypersensitivity
Min YANG ; Dongfeng CHEN ; Bo ZHANG ; Zhengguo WANG
Chinese Journal of Digestion 2010;30(9):610-613
Objective To investigate the effect of nociceptin/orphanin FQ (N/OFQ) on crossorgan sensitization and bladder function disorders in rat model with visceral hypersensitivity. Methods The model of cross-organ sensitization was induced by basic OVA-sensitization combined with colon mustard oil instillation in 44 female SD rats. All rats were then divided into control group (n= 14,infusion with 0.9% NaCl solution), model group (n= 14) and N/OFQ group (n= 16, infusion with N/OFQ). The anti-nociceptive effect of N/OFQ were evaluated by using the visceromotor response (VMR) to grade bladder distention (0. 5~2.0 ml, 20 s). Bladder function was evaluated with the cystometry by measuring voiding pressure and micturition frequency. Results The rats in model group had a significant increase of VMR response at 1.0 ml, 1.5 ml and 2.0 ml of pressure when compared to those in control group (P=0. 006,0. 015 and 0. 0331, respectively). However, N/OFQ treatment was able to reverse the increased VMR response in N/OFQ group at 1.5 ml and 2.0 ml of pressure (P=0. 016 and 0. 031, respectively). It was showed that mustard oil significantly reduced bladdervoiding pressure (P<0. 01) and significantly increased micturition frequency (P<0.01)in model group when compared to control group. The N/OFQ reversed the decrease of voiding pressure (P=0. 0038) and the increase of micturition frequency in N/OFQ group( P = 0. 001). Conclusions These findings indicate that the rat model of cross-organ sensitization was able to be established successfully by the basic OVA-sensitization combined with colon mustard oil instillation. N/OFQ has an antinociceptive effect on cross-organ hypersensitivity and attenuates changes in bladder urodynamic function.
7.Research progress in diagnosis and treatment of cystitis glandularis
Xiujia WANG ; Zhi QU ; Fengbo ZHANG ; Zhengguo JI ; Peiqian YANG
International Journal of Surgery 2021;48(3):207-211
Cystitis glandularis is a hyperplastic disease originated from the bladder mucosa, cystitis glandularis is a rare clinical disease, there is no standard diagnosis and treatment. The etiology and pathogenesis of cystitis glandularis are still unknown, it can be diagnosed according to clinical manifestations, laboratory and auxiliary examinations, and the diagnosis of cystitis glandularis mainly depends on pathological results. Cystitis glandularis has a cancerous tendency. The clinical treatment methods of cystitis glandularis include conservative treatment, surgical treatment and surgical combined with drug therapy, but different types of treatment methods are different, and most patients are treated by surgery combined with drug perfusion of the bladder.This article will review the research progress in diagnosis and treatment of cystitis glandularis.
8.Two-dimensional numerical analysis of impact response of the human tibia in Car-pedestrian accidents
Haibin CHEN ; Xuemei CHENG ; Deyuan LI ; Kai XIAO ; Guangyu YANG ; Zhengguo WANG
International Journal of Biomedical Engineering 2011;34(3):167-170
Objective The purpose of this paper was to use a new biphasic poroelastic tibia model to develop a two-dimensional numerical method for simulating impact responses of human tibia in car-pedestrian accidents. Methods The geometry of tibia model was reconstructed from CT scans of the left tibia of a living human volunteer. A poroelastic approach was utilized to establish the governing equations of the model and the finite element method was applied to solve these governing equations. Both cortical and cancellous components of tibia were represented using a poroelastic material model consisting of solid phase (matrix) and fluid phase (marrow). A lateral-medial impact direction was selected in the simulation analysis and the impact responses of the pedestrian tibia during 0-200 ms were analyzed. Results The bending deformation of the tibia predicted by the computer simulation was primarily concentrated on the impact zones. The displacement response of Node 107 in the impact zone indicated a peak displacement of -6 mm at around 75 ms, and the significant time delay between the impact force and the displacement response of the skeleton. The axial stress response at the center of element E77 in the impact zone indicated a peak stress of 140 MPa at around 30 ms,and the significant time delay was observed between the impact force and the axial stress response of the skeleton, too. Conclusion This research developed a two-dimensional numerical method for simulating impact responses of human tibia in car-pedestrian accidents. It was able to approximately simulate the bending deformation, lateral displacement response and axial stress response of pedestrian tibia in the impact zones,and the effects of the fluid phase on the solid phase. More in-depth investigation is helpful to further the biofidelity of tibia dynamics model.
9.Quantitative study of cochlear hair cells injuries in guinea pigs exposed to blast underpressure
Chaojun LI ; Zhaohua LIU ; Peifang ZHU ; Zhengguo WANG ; Cheng YANG ; Haibin CHEN ; Jihong ZHOU ; Xin NING
Chinese Archives of Otolaryngology-Head and Neck Surgery 2006;0(03):-
OBJECTIVE To explore the characteristics of cochlear hair cell injuries in guinea pigs exposed to blast underpressure(BUP). METHODS The guinea pigs were killed 14 days after exposure to experimental BUP. Their basilar membranes were stained by silver nitrate and the hair cell injuries were quantitatively assessed using the light microscope. RESULTS The outer hair cells(OHCs) of the guinea pigs clearly appeared to be injured following exposure to the experimental BUP at a peak underpressure varying between -22.4kPa and -63.3kPa. The most obvious injury was in the second turn and OHC loss was smallest in the first row and more severe in the second and third rows. OHC loss was seen in all of three experimental groups of animals following exposure to BUP. Furthermore, the higher the peak of underpressure, the more obvious the injury of OHCs. Quantitative morphological analysis of cochlear hair cells showed that the total OHC loss rates in all the experimental groups of guinea pigs exposed to BUP were significantly higher than that of control group animals(P
10.Percutaneous nephrostolithotomy with ballon dilation treated staghorn calculi in 89 cases
Zhengguo JI ; Yongqian CHEN ; Zhi LIU ; Jinming WANG ; Peiqian YANG ; Ye TIAN
International Journal of Surgery 2015;42(9):628-630
Objective To investigate the efficacy of percutaneous renal access with balloon dilation for staghorn calculi.Methods Eighty-nine cases with PCNL were enrolled from February 2012 to March 2015.Clinical data including the time for setting the renal access, operation time, residual stone rate, complications were analyzed.Results Eighty-nine cases established nephrostomy tracts successfully.The average time for setting the renal access was (5.7 ± 1.0) min (4-8 min).The average of operation time was (62.6 ± 14.1) min (37-87min).The average of Hemoglobin decline rate was (6.3 ± 2.5)% (2.8%-16.9%).The residual stone rate was 12.5%.Conclusions PCNL with ballon dilation is a fast, safe and effective means for staghorn calculi.It is worth using for staghorn calculi.