1.PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODY AGAINST SYNTHETIC PEPTIDES OF HUMAN TOLL-LIKE RECEPTOR 4
Qingwu YANG ; Peifang ZHU ; Zhenggu WANG
Medical Journal of Chinese People's Liberation Army 1982;0(01):-
An immunogenic peptide sequence of B cell dominant epitope of human Toll like receptor 4 (TLR4) was selected basing on predication of B cell epitope of TLR4.bALB/c mice were immunized with synthetic peptide of TLR 4 and one clone (B4) of hybridoma cells was picked out by ELISA and limited dilution. High titer McAb prepasations against TLR 4 were obtained from ascites fluid of BALB/c mice infravenously inoculated with the McAb inducing hybridoma cells.The titer of ascites fluid was 1∶ 100 000 .The subclass of the McAb was IgG2,k.The specificity of McAb was identified. by indirect ELISA,and a single strong blot was shown in molecular weights of 90 Kd.using Western blot analysis.
2.PROTECTIVE EFFECT OF PYRROLIDINE DITHIOCARBAMATE(PDTC) ON ACUTE LUNG INJURY(ALI) INDUCED BY BLUNT CHEST TRAUMA COMBINED WITH LPS CHALLENGE IN RATS
Kelong LIAO ; Peifang ZHU ; Zhenggu WANG
Medical Journal of Chinese People's Liberation Army 1982;0(01):-
To explore the protective effect of pyrrolidine dithiocarbamate (PDTC) on acute lung injury induced by blunt chest trauma combined LPS challenge in rats. The combined injury model was reproduced by blunt impact on the right lateral superior chest of rats with 350 kPa drive pressure inflicted with a small multi function bio impact apparatus, folloned by an injection of LPS (5mg/kg) into the fail vein. PDTC (120mg/kg) was injected intravenously fore injury in animals of the PDTC pretreatment group. The 48 hour mortality rate was recorded, myeloperoxidase (MPO), NF ?B activity, and TNF?, IL 8 mRNA expression and their proteins of lung tissue were examined. The results showed that PDTC obviously reduced the 48h mortality the activity of MPO and NF ?B, and the gene expression and protein crotein contents of TNF?and IL 8,suggesting that PDTC has a protective effect on the combined injury. This effect might be associated with its inhibitory effecton NF ?B activation and expression as well as the release of proinflammatory cytokines.
3.CHANGES IN NO IN BRAIN TISSUES AND ITS RELATIONSHIP WITH NEUROBEHAVIORAL FUNCTION AFTER WHOLE BODY BLAST INJURY
Jianxin JIANG ; Cernak IBOLJA ; Zhenggu WANG
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
This study was to investigate the changes in NO - 2/NO - 3 content in the hippocampus and brainstem and its relationship with neurobehavioral alteration after whole body blast injury (WBBI) in rats. It was found that the contents of NO - 2/NO - 3 and cGMP in the hippocampus and brainstem were significantly increased 3, 24 and 120h after WBBI, and they were markedly correlated with blast injury severity. Activeavoidance response (AAR) decreased obviously, and active avoidance response latency (AARL) and escape response latency (ERL) were significantly prolonged after WBBI. The correlation analysis indicated that the changes in NO - 2/NO - 3 in brain tissues were also significantly correlated with AAR, ERL and AARL respectively. The results suggested that NO production was significantly increased in the hippocampus and brainstem after whole body blast injury. The over release of NO might be related to the blast wave induced damage to the central nervous system.
4.CHANGES IN PULMONARY INTERCELLULAR ADHESION MOLECULE-1 AND ITS SIGNIFICANCE AT THE EARLY STAGE OF BURNS
Rongbing JIN ; Peifang ZHU ; Zhenggu WANG
Medical Journal of Chinese People's Liberation Army 1983;0(05):-
To investigate the role of ICAM 1 in the aggregation of polymorphoneucleocytes (PMN) in the lungs at the early stage of burns. Myeloperoxidase (MPO) content in lung tissues and bronchoalveolar lavage fluid (BALF) was assayed. Intercellular adhesion molecule 1 (ICAM 1) and its mRNA expression in lung tissues were determined by immunohistochemical method and in situ hybridization. CD11b/CD18 expression on the circulating PMN were measured by flow cytometry. The results showed that levels of MPO in lung tissues and BALF were markedly higher than those of control after burn injury. Expression of ICAM 1 and its mRNA in the lung tissues and CD11b/CD18 on circulating PMN surface were significantly increased at 2, 6, 12, 24hr after burns. It suggests that PMN aggregation in the lungs might be related to increased ICAM 1 expression on pulmonary microvascular endothelial cells and CD11b/CD18 expression on PMN at the early stage of burn injury.