Objective To observe the expression and distribution of the basic fibroblast growth factor(bFGF)in lung through chronic obstructive pulmonary disease(COPD)rat models and to investigate the potential role of bFGF in the mechanism for airway remodeling in COPD.Methods Forty male,8 weeks old Wistar rats were divided into groups randomly:COPD group;bFGF interfere in COPD group;anti-bFGF interfere in COPD group,and control group.COPD models were established by intratracheal instillation of lipopolysacchairde(LPS)twice in a month and exposure to cigarette smoking daily and in order to contrast respectively instillate particular quantity bFGF and anti-bFGF into different COPD model group from caudal vein in every week.After these models became true,observe the pathologic alteration of small airways,arterioles and pulmonary alveolus by hematoxylin and eosin stain Van-Gieson +elastic fibers stain.At the same time,the thickness of the smooth muscle and collagen in bronchi and pulmonary arterioles were measured by computer image analyzer;also the protein and gene relative content of bFGF as well as the effects of antibody on them were observed by Histostaining and ISH(in situ hybridization)detection.Results There was a significant increase in the thickness of the smooth muscle and collagen in bronchi of bFGF interfere in COPD group compared with that of control group and COPD group(P

Objective To investigate the effects of islet B cells of rat on the differentiation of T-helper lymphocytes and its potential mechanism.Methods Forty male SD rats were equally divided into five groups.Group-A:render diabetes by injection of streptozotocin (STZ);Group-B:OVA sensitized and then OVA challenged to render asthma;Group-C:render diabetes and then OVA sensitized,and challenged;Group-D:render diabetes,and then insulin subcutaneous injection,OVA sensitized,and challenged;Group-E:nondiabetes,nonsensitized,only OVA challenged.Bronchoalveolar lavage (BAL) was performed in rats from each group 24h after challenged and BAL fluid was used to detect IFN-? and IL-4.Blood glucose was estimated,total and differential leukocyte counts were carried out in blood samples and C-peptide concentration was tested in serum.Results The C-peptide concentration in serum was decreased significantly,while INF-? expression was higher,and IL-4 expression was markedly lower in BAL fluid in both group-A and group-C when compared with that of controls,respectively.Compared with group-C,IL-4 expression was increased and INF-? were significantly lower,respectively in BAL fluid of group-D after insulin subcutaneous injection.Conclusion Islet B cells of rat might modulate the differentiation of T-helper lymphocyte.

Objective To investigate the ability of osteogenesis, repaired effects and possible mechanism of tissue engineered bone made in an approach of bionics as a transplantation biomaterial to repair a segmental defect of long bone. Methods HA/?-TCP was composed with PDLLA and then composed with rhBMP-2 and collagen of typeⅠ. The combined biomaterial was put in common culture with osteoblasts harvested from periosteum of rabbit and vascular endothelial cells from kidney of rabbit then transplanted this tissue engineered bone to total segmental periosteum-bone defect of 1.5 cm in the rabbits radius which were investigated 4, 8 and 12 weeks after operation respectively. Investigation of the bone defect was made by means of gross observation, X-ray examination, histology of HE and Masson staining, image pattern analysis, scanning electron microscopy, EDAX. Results In gross observation, the implantations were adhered to the host bone well in four weeks, the implantations was bony healed with host bone in eight weeks, and some of the implantations were replaced by new formation bone in 12 weeks. In histological examination of four weeks after operation, lamellar bone was found, and eight weeks after operation, implant was incorporated to host bone end by end through cortical bone, and new bone marrow was found to invade into the implant. Furthermore, the outer part of implant was completely substituted by new cortical bone 12 weeks after operation. In addition, the histological study pointed out that the new bone arranged in type of various bands which were in subsequent transition. There is significant difference between 4 weeks and 8 weeks, and 4 weeks and 12 weeks, but no significant difference between 8 weeks and 12 weeks of the quantity of new bone. The ratio of calcium to phosphorus in the transplants tended to approach that in the host cortical bone along with the period of time after operation. Conclusion Satisfied effects of remodeling appeared after this tissue engineered bone composed by bionics was transplanted to the segmental defect of long bone. The mechanism of bone regeneration was endochondral ossification.