Objective:To study the effects of cells ’ and bacteria ’s adhesion and proliferation on different fiber diameters of polypyrrole coating with electricity.Methods:Titanium coated with polypyr-role was divided into no electrical stimulation and stimulation groups,each group had 30-60 nm,70-1 00 nm,1 30-1 70 nm diameters of the fiber.MC3 T3 cells and Staphylococcus aureus (S.aureus)were inoculated on different fiber diameters of polypyrrole coating with and without electric stimulation .We gave the electrical stimulation group 1 00 mV for 1 h and every 24 hours gave it 1 h stimulation,and no e-lectrical stimulation group was not managed.We used scanning electron microscope (SEM)to observe the cells’and bacteria’s morphology.The cells were given 20 mL CCK-8 solutions after 1 ,3,7 days’ cultivation,then incubated for 2 h,the solution was transferred to 96-well plate,we measured the cells’ CCK-8 of the 30-60 nm,70-1 00 nm,and 1 30-1 70 nm groups by Elisa.The cells on different fiber diameters were also stained by live-dead cell staining kit,TritonX-1 00 and DAPI.We used PBS to wash and glycerin to seal them.The live-dead situation and morphology were tested by co focal microscope. The bacterial were stained by Live/dead baclight bacterial viability kits,we detect the suspension’s D of the 30-60 nm,70-1 00 nm,and 1 30-1 70 nm groups,and also observed the bacteria’s survival situa-tion by co focal microscope.Results:The CCK-8 of the cells with direct current stimulation was higher than that of the unpowered group (F=1 2.248,P=0.006).The smaller the fiber diameter,the better was the cell’s adhesion and proliferation (F=9.261 ,P=0.005).The bacterial suspension’s D of the electric group was lower than that of the unpowered group,and the fiber diameter had no significant effect on the bacteria’s growth(F=9.641 ,P=0.036).Conclusion:Polypyrrole coating with electricity can promote the cell’s proliferation and inhibit the bacteria’s proliferation,and the cell growth on small fiber diameter coating is better.There is no difference in the bacterial growth of different fiber diameter coatings.

Age-related ovarian hypofunction includes a decrease in follicle quantity and quality as well as alterations in the ovarian microenvironment，the mechanisms of which are mainly related to mitochondrial dysfunction，free radical and antioxidant systems，telomere and telomerase alterations，and apoptosis，and is one of the major factors contributing to infertility in advanced maternal age （AMA）. Despite the tremendous progress in assisted reproductive technology in recent decades，few breakthroughs have been made in alleviating age-related ovarian hypofunction and improving reproductive outcomes for AMA. In recent years，there has been an increasing number of studies on the multi-level and multi-targeted mechanisms of traditional Chinese medicine （TCM） to improve age-related ovarian hypofunction by modulating mitochondrial homeostasis，alleviating oxidative stress，and inhibiting apoptosis，while more high-quality randomized controlled trials have demonstrated the clinical efficacy of TCM in assisted reproductive technology. Given this，this article presented a systematic review of recent research and randomized controlled trials on the mechanism of Chinese medicine active ingredients，single Chinese medicine， and Chinese medicine compounds in delaying age-related ovarian hypofunction，to clarify the current status and shortcomings of the research. This paper provides medication management of TCM for effectively alleviating age-related ovarian hypofunction and improving reproductive outcomes for AMA.