Objective:To evaluate alteration of sexual function and defecation after hysterectomy with two different operation techniques. Methods: Total hysterectomy was performed in 31 patients(groupA) ,and inlrafascial hysterectomy was carried out in 31 patients. Alterations of sexual function and defecation after operation were investigated by using a questionnaire. Results : The incidence of anxiety and depression during intercourse was 50. 8% ; 45% of patients complained of decreased libido and decreased quality of coitus. There existed no signficant difference between group Aand B. The defecation habit was altered in 45% of cases. The incidence in group A was higher than in group B. Conclusion :The hysterectomy led to alteration of sexual function . It was necessary to give guidance of sexual surgery technique and intrafascial hysterectomy was a better choice.

Objective:To study the effects of MRI in the follow-up after HIFU.Methods:The tumor-like proliferation of monkey uterus was treated with high intensity focused ultrasound(HIFU)then followed up with magnetic resonance imaging (MRI) to observe the change of signals of treated region.Results:The blood flow signals were not observed in treated region with MRI after HIFU.The volume of treated region decreased gradually,which was consistent with the results of pathology and B-ultrasound.Conclusion:MRI is efficient in follow-up for the change of signals of treated region after HIFU and can reflect the histological change of treated region precisely.

Objective To investigate the main symptoms and the health care consciousness of the menopausal women.Methods From Jul.2013 to Oct.2014,a one-on-one,face-to-face questionnaire was conducted in selected menopausal women in Gynecology Clinic of the Second Affiliated Hospital of Chongqing Medical University.Results There were 583 valid questionnaires overall.349 women had natural menopause,and the average age was(48.7±3.4) years.66.72％ women appeared menstrual disorders in premenopausal stage,presenting longer cycle and fewer menstrual quantity.82.50％ (481/583) women had menopausal symptoms,with hot flashes (57.46％,335/583) as the most,followed by insomnia (50.60％,295/583).Kupperman score showed 7.03％ (41/583)patients were the severe degree.Among the patients with menopausal symptoms,234 cases went to menopause clinics on their own initiative and 36 cases visited other department first and then transferred to menopause clinics.Among patients with college or above degree,51.27％ (101/197)received menopausal hormone therapy (MHT),significantly higher than those with poor educational background.Conclusion Vasomotor symptoms such as hot flashes were still the main symptoms of menopausal syndrome.Although some menopausal patients had intentions to seek medical care by themselves,there sre still some patients lacking health care consciousness,which is more commonly seen in patients with lower level of education.Menopausal education promotion is needed.

Objective to get the message of the state of refreser doctor patient communication and put forward the solution.Method 200 patients were investigated who were hospitalized in the department of gynaecology and obstetrics of the seconed affiliated hospital of Chongqing Medical University,and the state of the doctor patient communication as well as the affecting factor were questionnaired.Results 182 questionnaire was reclaimed,the ratio of reclaim was 91%.50.3% patient considered the advanced study doctor communation was satisfactory,41.2% patient considered the communicatin is common,8.5% patient considered the communicatin is dissatisfing.65.3% patient considered the service attitude affected the communication,42.3% patient considered the speech of the doctor effect the communication,41.2% patient considered the behavior of the doctor effected the communication.Conclusion the refresher doctor was deficient in the doctor patient communication,and the training was required in the service attitude,speech and behavior arts.

0.05).But the expression of Caspase-9 in AC group was significantly higher than that in CIN and squamous cell carcinoma group(P

Objective To study the role of HMGA2 in the occurrence and the progression of uterine leiomyomas.Methods Fifty cases of uterine leiomyomas who met the inclusion criteria(aged 30-50 years old,free from hormone at least for 6 months before surgery,no other diseases,postoperative pathological diagnosis as uterine leiomyoma) admitted to our hospital from January to July 2003 were studied.The expression of HMGA2 mRNA in uterine leiomyomas and neighbour normal myometrium was detected by ISH.Results HMGA2 mRNA was strongly expressed in 30 cases of uterine leiomyomas but not expressed in neighbour normal myometrium.There was no difference in HMGA2 mRNA between proliferative stage and progestational stage.Conclusion The increased expression of HMGA2 in uterine leiomyomas may be important in the occurrence and the progression of uterine leiomyomas.

Objective To explore the feasibility,efficiency and effect of wide-type p53 gene transfected into cervical cancer HeLa cells by ultrasound microbubble intensifier. Methods Based on the optimum parameter of ultrasound wave irradiation (0.5 W/cm2 ,30 s) selected in pre-experiment,HeLa cells were divided into 5 groups:(A)simple plasmid group,(B)plasmid-ultrasound group,(C)plasmid-ultrasound-microbubble group,(D)plasmid-liposome group,(E)blank control group. At 24-48h after transfection,the transfection efficiency was detected by fluorescence microscope,the mRNA expression of p53 was detected by RT-PCR,the cell cycle was detected by flow cytometry,and the inhibition rate of cells growth was detected by MTT assay. Results Fluorescence microscopy showed that the expression of green fluorescent protein in group C was higher than that in group Band group D (P

Objective To assess the curative effect of different therapeutic regimen in perimenopausal and postmenopausal pa-tients with depressive disorder ,in order to guide clinical work .Methods Relevant literatures were collect by retrieving a number of online database ,,screen documents independent ,the randomized controlled trial(RCT) and cohort study were included ,and carried on the quality evaluation and data extractione ,then completed the Meta analysis .Results 19 papers were inclouded (2 312 cases) , including 10 RCT papers and 9 cohort papers .There was no statistically significant difference for cure rate (RR=1 .15 ,P=0 .07) , the difference of effective rate had statistically significant (RR=1 .17 ,P=0 .005) .Hormone in combination with antidepressants compared with hormone replacement therapy :the difference of cure ratio (RR=1 .73 ,P=0 .003) and effective rate (RR=1 .52 , P<0 .01) all had statistically significant .The difference of effective rate between antidepressants and hormone replacement therapy had statistically significant (RR=1 .37 ,P=0 .003) .Hormone replacement therapy compared with placebo :the difference of cure rate had statistically significant(RR=2 .17 ,P=0 .03) ,the difference of effective rate had no statistically significant (RR= 0 .78 ,P=0 .15) .Conclusion Although the hormone replacement therapy has a positive effect on the mood ,it′s promoting effect on the anti-depressant of perimenopausal and postmenopausal women is uncertain .

The article analyses the protrudent problems in teaching foreign students and put forward some countermeasures.

Objective To explore the feasibility of using irreversible electroporation (IRE)mediating HPV16 E6 shRNA into cervical cancer cell line SiHa,and to clarify the influence of their co-effect on the proliferation of SiHa cells and its mechanism.Methods A HPV16 E6 gene specific interference sequence was inserted in pGenesil-1 to build a interference vector.10 pulses of IRE with 800 V,100 μs,and 1 Hz were applied to the suspension of SiHa cells and vectors.According to the treatment factors,control group,IRE group,pGenesil-N group,pGenesil-N+IRE group,pGenesil-E6 group and pGenesil-E6 + IRE group were set up.The expression of green fluorescent protein (GFP)and transfection efficiency were confirmed by inverted fluorescence microscope 24 h after the vector was transfected by IRE,and the expression efficancy of GFP was calculated.The expression levels of E6 mRNA and protein were detected by RT-PCR and Western blotting method which was also applied to detect the expressions of P53 and PCNA.The proliferative activity of SiHa cells was determined by CCK-8 assay.Results Enzyme digestion and DNA sequencing verified that the vectors were correctly constructed.GFP was seen under inverted fluorescence microscope 24 h after IRE transfection.Compared with IRE group,the expression levels of E6 mRNA and protein were decreased detected by RT-PCR and Western blotting method after the vectors were treated with IRE,the P53 protein expression level was increased (P < 0.05),and the PCNA expression level was decreased (P <0.05).The CCK-8 assay results showed the proliferative activity of SiHa cells in pGenesil-E6+IRE group was decreased more obviously than that in pGenesil E6 group (P <0.05).Conclusion IRE can play the role of gene transfection of mediating HPV16 E6 shRNA into SiHa cells, and their co-effect can significantly inhibit the proliferation of SiHa cells.