China ; epidemiology ; Humans ; Occupational Diseases ; epidemiology ; prevention & control ; Surveys and Questionnaires ; Trimethyltin Compounds ; analysis ; poisoning

Chromatography, Gas ; Humans

Air ; analysis ; Chromatography, Gas ; methods ; Ethylene Oxide ; analysis ; Furans ; analysis ; Methylene Chloride ; analysis ; Workplace

This article described the concept of the professional ethics and discussed the significance of the existence and construction of professional ethics on medical journal editors. The professional ethics on medical journal editors could be beneficial to correctly understand the ethical problems of medical journal editors and to promote the medical journal editors' role localization. It is very important to construct the Standardization of medical journal editors' behavior.

Objective To explore the residual undifferentiated mouse embryonic stem cells (ESCs) in embryoid bodies. Methods Mouse R1 and Oct-4-GFP transgenic ESCs were firstly cultured in suspension to form embryoid bodies (EBs). Twenty days later, EBs were digested into single cells and then re-plated in standard ESC culture condition. The morphology of residual undifferentiated cells in EBs was observed, and surface makers and in vitro redifferentiation potency of residual cells were examined by flow cytometry and immunofluoreseent staining. The residual cells were expanded and subcutaneously injected into nude mice, and the specimens were harvested from the injection site for histological analysis 6 weeks after injection. Results There were residual undifferentiated ESCs in EBs differentiated for 20 days, which displayed clonal morphology and expressed undifferentiated cell markers of ESCs, including SSEA1, CD31, CD9 and Oct-4. The cells could be differentiated to form EBs again, and could be re-expanded from secondary EBs. The residual cells were able to form teratoma at the injection site, and mature endoderm, mesoderm and ectoderm tissues could be found in teratoma tissues. Conclusion There are residual undifferentiated ESCs after differentiation of ESCs into EBs. The residual ESCs can differentiate again in vitro and in vivo, and can residue again in the in vitro differentiation.

OBJECTIVETo explore the hepatic toxicity and the exposure biomarkers of N, N-Dimethylacetamide.

METHODSOne hundred forty five objects were chosen by stratified random sampling method. The investigation was performed using questionnaire and physical examination. The air concentrations of DMAC in the workshops were monitored. The urine samples were collected and analyzed after work everyday or at the weekend. The correlation between the air concentrations of DMAC in the workshops and the concentrations of urinary NMAC wee analyzed by regression.

RESULTSThe air concentration of DMAC in the spinning workshop was higher than others. The morbidity of abnormal hepatic function was 12.4%, 61.1% of workers with abnormal hepatic function appeared in one year after exposure to DMAC in the workshops ( r=0.44, P<0.01).

CONCLUSIONThe abnormal heptic function was found in workers exposed to DMAC for short period. The concentration of urinary NMAC can serve as the exposure biomarker of DMAC.

Acetamides ; toxicity ; urine ; Adolescent ; Adult ; Air Pollutants, Occupational ; analysis ; Biomarkers ; urine ; Environmental Monitoring ; Humans ; Liver Function Tests ; Male ; Middle Aged ; Occupational Exposure ; Surveys and Questionnaires ; Workplace ; Young Adult

microRNAs (miRNAs) are a class of non-coding RNAs that function as endogenous triggers of the RNA interference pathway. Studies have shown that thousands of human protein-coding genes are regulated by miRNAs, indicating that miRNAs are master regulators of many important biological processes, such as cancer development. miRNAs frequently have deregulated expression in many types of human cancers, and play critical roles in tumorigenesis, which functions either as tumor suppressors or as oncogenes. Recent studies have shown that miRNAs are highly related with cancer progression, including initiating, growth, apoptosis, invasion, and metastasis. Furthermore, miRNAs are shown to be responsible for the cancer-related inflammation, anti-cancer drug resistance, and regulation of cancer stem cells. Therefore, miRNAs have generated great interest as a novel strategy in cancer diagnosis and therapy. Here we review the versatile roles of miRNAs in cancers and their potential applications for diagnosis, prognosis, and treatment as biomarkers.
Animals ; Biomarkers, Tumor ; Drug Resistance, Neoplasm ; genetics ; Epithelial-Mesenchymal Transition ; genetics ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; Genes, Tumor Suppressor ; Humans ; Inflammation ; genetics ; MicroRNAs ; genetics ; physiology ; Neoplasm Invasiveness ; genetics ; Neoplasm Metastasis ; genetics ; Neoplastic Stem Cells ; metabolism ; Oncogenes ; genetics

OBJECTIVETo investigate possible differences in the prognosis in children with severe nocturia who received different drug withdrawal schedules.

METHODSNinety-seven children with severe nocturia were randomly assigned to two groups: control (n=47) and observed (n=50). The control group accepted drug withdrawal immediately, while the observed group accepted dose tapering gradually after a 12-week treatment course. The frequency of enuresis was observed three months after complete drug withdrawal.

RESULTSDuring the treatment, the frequency of enuresis in all of children from both the control and the observed groups was reduced by over 90%. Forty-six children (92%) from the observed group showed the frequency of enuresis was reduced by over 90%, but 28 children (60%) from the control group (p<0.01) three months after the complete drug withdrawal. There were no significant differences in the adverse effect and the medication compliance between the two groups.

CONCLUSIONSThe different schedules of drug withdrawal may lead to different prognosis, and the schedule of gradual drug withdrawal may be superior to the immediate one in children with nocturnal enuresis.

Adolescent ; Child ; Deamino Arginine Vasopressin ; administration & dosage ; Drug Administration Schedule ; Female ; Humans ; Male ; Nocturnal Enuresis ; drug therapy ; etiology

Acute Disease ; Adult ; Altitude Sickness ; blood ; enzymology ; Glutathione ; blood ; Glutathione Peroxidase ; blood ; Humans ; Lipid Peroxidation ; Male ; Military Personnel ; Nitric Oxide ; blood ; Nitric Oxide Synthase ; blood ; Oxidoreductases ; metabolism ; Superoxide Dismutase ; blood

OBJECTIVETo establish a detection method for trimethyltin chloride in urine by the Head space-GC.

METHODAfter derivatizing trimethyltin chloride, the urines was separated by the head space-gc, and then the trimethyltin chloride detected qualitatively and quantificationally.

RESULTSIn the concentration range of 0.02 ∼ 0.40 mg/L urinary trimethyltin chloride, showed a quadratic, r = 0.9992, detection limit was 0.005 mg/L, the relative standard deviation was 1.9% ∼ 2.5%, recovery was 92.0% to 100%, the urine samples can be saved at least 90 days in -18°C refrigerator.

CONCLUSIONThe instrument, reagents involved in the detection require low, the operations to processing samples are simple, high sensitivity, less interference, good reproducibility, and suitable for quantitative and qualitative analysis, convenient to promotion.

Chromatography, Gas ; methods ; Humans ; Trimethyltin Compounds ; urine ; Urinalysis ; methods