Objective To explore the effects of vecuronium bromide within 24 hours in severe bronchial asthma with acute attack with breathing machine in conventional sedatio.Methods Prospective study,double-blind randomized standard control,where 46 cases of patients of severe bronchial asthma with acute attack transferred to ICU using breathing machine in this year were randomly divided into the vecuronium bromide group (muscle relaxant group) and conventional sedation group (normal group) according to medical record number; Two groups of sample compositions were compared by x test,using two independent samples of t test to compare the effects the treatment and prognosis.Results After mechanical ventilation for 15min and 1-3h in muscle relaxant group,patients' vital signs were statistically analyzed,including respiration; Blood gas analysis parameters were as follows:pH,PaO2,PaCO2,SpO2 of these were improved compared with those in the conventional group (P ＜ 0.05) with statistically significant differences;Compared with the conventional group,in the muscle relaxant group,the retention time in ICU (146.20 ± 17.92) hours,(214.15 ± 22.21) hours,the using time of breathing machine (125.93 ± 16.23) hours,(192.89 ± 22.60) hours,and the dosage of glucocorticoid (2.61 ± 0.17) mg · kg-1 · d-1,(3.55 ± 0.26) mg · kg-1 · d-1,anticholinergic agent(6.25 ±0.51) μg · kg-1 · d-1,(10.64 ±0.75) μg · kg-1 · d-1 and β2 adrenergic receptor agonist (0.06 ±0.01)mg · kg-1 · d-1,(0.15 ±0.04)mg · kg-1 · d-1 were all reduced (all P ＜0.05) with statistically significant differences;The patients were smoothly transferred out of ICU,without the adverse events of mechanical ventilation and cardiovascular diseases.Conclusion The use of vecuronium bromide made the patients with severe bronchial asthma in routine sedation use of breathing machine rapidly correct the clinical symptoms and improve the off-period prognosis.

0.05).Conclusion The therapeutic mechanism of AEP of Chinese herbal medicine for asthma may be related with the inhibition of EOS infiltration,the decrease of peripheral IL-4 content and the increase of IFN-?.

0 05). The levels of TC, LDL-C and apoB in the patients with CSX were significantly higher than those in the patients with CN(P

1 000 rnl were randomly divided into 2 groups : AHHD group ( n = 18) and control group ( n = 18). The patients were premedicated with intramuscular atropine 0.007-0.01 mg?kg-1 . Radial artery and right internal jugular vein were cannulated before induction of anesthesia for MAP and CVP monitoring, blood sampling and fluid administration. Anesthesia was induced with TCI of propofol. The target plasma propofol concentration was set at 3 ?g ? ml -1 . When the patients lost consciousness, fentanyl 2 ?g ? kg-1 was given intravenous and tracheal intubation was facilitated by vecuronium 0.1 mg? kg-1 , 10min after intubation additional fentanyl 2 ?g? kg-1 and vecuronium 0.08 mg? kg-1 were given. In AHHD group lactated Ringer's solution 10 ml ? kg-1 was infused over 30 min before TCI propofol was started. 10 min after start of TCI propofol 6% HES 20 ml? kg-1 was infused within 30 min. In control group the patients received only lactated Ringer's solution 10 ml?kg-1 . TCI of propofol was maintained for 1 h. Arterial blood samples were taken before and 2, 5, 10, 20, 30, 40, 50 and 60 min after TCI propofol was started, and at 2.5, 5, 10, 15, 20, 25, 30 min after termination of TCI propofol for determination of blood concentration of propofol by gas-chromatography - mass spectrometry (GC-MS) . The TCI system comprising Graseby 3 500 infusion pump controlled by Stelpump 1.07 software which included Tackley pharmacokinetic parameters. Results In AHHD group Hb was reduced from initial (130? 14)g?L-1 to (90? 15)g?L-1 and Hct from initial 38% ? 3% to 26% ? 4 % at the end of AHHD. The measured blood propofol concentrations were significantly lower in AHHD group than those in control group at the corresponding time points (P

Obuective To assess the population pharmacokinetic parameters and analyze thecharacteristics of pharmacokinetics of propofol given by target-controlled infusion (TCI) in Chinese using anoulinear mixed effect model (NONMEM) program. Methods Sixty-one ASA Ⅰ-Ⅱ patients (26 male, 35female) aged 18-64yr, weighing 41-83kg undergoing elective operation under general anesthesia were studied. TheTCI system consisted of (1 ) Stel pump Software 1 .07 designed by Coetzee, (2) cable R232 connector, (3)Graseby 3500 infusion pump, (4 ) pharmacokinetic parameters developed by Tackley. The patients werepremedicated with intramuscular phenobarbital sodium 1-2 mg?kg~(-1) and atropine 0. 5 mg. Anesthesia was inducedwith TCI of propofol. Target plasma concentration of propofol was set at 3?g?ml~(-1). Fentanyl 2?g?kg~(-1) andvecuronium 0. 1mg?kg~(-1) were given i. v. when the patients lost consciousness. TCI of propofol lasted 60 min. 976blood samples were obtained before induction of anesthesia and at 2, 5, 10, 20, 30, 40, 50, 60, 62. 5, 65, 70,75, 80, 85, 90 min ther TCI was started for determination of plasma propofol concentration by gas-chromatography-mass spectrometry (GC-MS). Population pharmacokinetic parameters were assessed and thecharacteristics of the pharmacokinetic profile was analyzed using NONMEM program. Results The pharmacokineticprofile of propofol given by TCI in Chinese was best described by an open two-compartment model. Thepharmacokinetic parameters for the final model: K_(10) was 0.111, K_(12) 0 .064 and K_(21) 0 .023 min~(-1); V_1 was 0 .205and V_2 0.404 L?kg~(-1); CL_1 was 22 .76 and CL_2 13 .24 ml?min~(-1). The estimated concentrations were well correlatedwith the measured concentrations in the final model. Weight was found to covariate significandy with V_1 and CL_1and age with K_(21). However gender had no significant effect on pharmacokinetic parameters.Conclusion Thepopulation pharmacokinetic profile of propofol administered by TCI in Chinese can be well described by an open two-compartment model. The volume of central compartment was smaller and the inter-compartmental transfer ratefrom central compartment to peripheral compartment was faster in Chinese.

Objective To investigate the effect of acute hypervolemic hemodilution (AHHD) on pharmacokinetics of propofol given by target-controlled infusion (TCI). Methods Thirty-six ASA Ⅰ-Ⅱpatients (18 male, 18 female) undergoing elective surgery were randomized to one of two groups: control group (n = 18) and AHHD group ( n = 18). The patients were premedicated with atropine 0.007-0.01mg?kg-1 and phenobarbital 1-2mg?kg-1 i.m. . Radial artery and right internal jugular vein were cannulated before induction of anesthesia. Anesthesia was induced with TCI of propofol. The target plasma propofol concentration was set at 3 ?g?ml-1. When the patients lost consciousness, fentanyl 2?g? kg-1 was given i.v. and tracheal intubation was facilitated by vecuronium 0. 1 mg?kg-1 . Ten minutes after tracheal intubation an additional dose of fentanyl 2 ?g?kg-1 and vecuronium 0.08 mg?kg-1 was given i.v.. TCI of propofol continued for 1 hour. In AHHD group lactated Ringer's solution 10 ml?kg-1 was infused over 30 min before induction of anesthesia. 10 min after TCI propofol was started, 6 % HES 20 ml ?kg-1 was infused within 30 min. In control group the patients received only lactated Ringer's solution 10 ml? kg-1 . All fluid infused was prewarmed to 35℃ Arterial blood samples were taken before and 2,5, 10, 20, 30, 40, 50 and 60 min after TCI propofol was started and 2.5, 5, 10, 15, 20, 30 min after termination of TCI propofol for determination of blood concentration of propofol by gas-chromatography-mass spectrometry (GC-MS) . The TCI system consisted of Graseby 3500 infusion pump controlled by Stelpump 1.07 software, which included Tackley pharmacokinetic parameters. Results The demographic data including sex, age, body weight and amount of propofol consumed were comparable between the two groups. The pharmacokinetic profile of propofol given by TCI was best described by a two-compartment open model during AHHD. The pharmacokinetic parameters tor the final model; K10 was0.116, K12 0.0907 and K210.024mm-1 ; V, was 0.311 and V2 0.446L?kg-1 ; Cl1 was 33.31 and Cl2 16.65 ml?min-1?kg-1 respectively. V1 and V2 were significandy larger, and transfer and clearance rates were significantly higher in AHHD group than those in control group. At the end of AHHD, Hb decreased by 31.0% and Hct by 31.3%; total plasma protein decreased by 30.1% and plasma albumin by 25.7% as compared with the baseline values before AHHD. Conclusion AHHD has significant effect on pharmacokinetics of propofol. Less propofol is bound to plasma protein and duration of action is relatively shorter. During AHHD the target plasma propofol concentration should be increased to some extent to achieve the same depth of anesthesia.

Objective To explore the relationship between respiratory viruses infection and the episode of steroid responsive simple nephrotic syndrome(SRSNS).Methods Thirty-eight children with SRSNS were selected,including the active stage group(n=28)and the remission group(n=10).The urine from 18 cases of nephritic nephrosis,16 cases of bronchiolitis,15 cases of secondary glomerular diseases and 15 cases of healthy children were served as controls.By using the method of reverse transcriptase-polymerase chain reaction(RT-PCR)and alkaline phosphoesterase-anti alkaline phosphoesterase enzyme-linked assay(APAAP),viral genes and antigens in urine were assayed,respectively.Results The positive rate of detecting viruses in the active stage group of SRSNS children was higher than that in the remission group and the other control groups(Pa

Objective To investigate the clinical festures and CLCN1 gene mutation screen on a myotonia congenital kindred.Methods The clinical data of 22 patients in the myotonia congenital kindred were analysed. Results There were total 68 people in 5 generations, including 24 patients in 4 generations .Both male and female were suffered. All patientes of this kindred showed myotonia with onset the illness from the infant, and 16 cases accompanied with hypermyotrophy.The levels of creatases and dielectric in serum were normal in all the cases. Spontaneous myotonic electric potential were observed on electromyography( EMG ) in 2 cases.The proband was found in light microscope by biopsy that muscle fibers arranged loosenly , size of them mismatched , transverse striation was unclear and some of them was hyperplasia and hypertrophy. Muscle cells degenerated gently with a few inflammatory cells infiltration. No mutation was found in the whole 23 extrons of CLCN1 gene in the 3 patients.Conclusions This kindred accords with the autosomal dominant heredity form Thomsen's disease. The affected numbers have the typical clinical characteristics. No mutation is found when 23 extrons of CLCN1 gene screened in the patients which indicate the genetic heterogeneity may be exist in this kindred.

Objective An investigation on social function cognition for bedside care service at patient's home was conducted in the residents aged over SS years in Jingshan Community,Dongcheng District,Beijing,to lay a scientific basis for provision of it.Methods Ninety-seven residents aged over 55 years were selected from 58 families with systematic sampling in Jingshan Community by a questionnaire interview,including history of illness,income,medical expense,awareness of bedside service at patient's home,etc.Results Totally,98.9 % of the residents suffered from at least one kind of chronic disease,98.9 % of them never received bedside care service at their homes,94.9% of them only knew a little of such kind of health care service,91.85% of them knew some social function of it,and 84.5 % of them did not know the criteria of payment for the service.Conclusions Publicity of bedside care service at patient' s home should be strengthened,focusing on its social function,inexpensiveness,convenience,etc.Function of bedside service should be perfected further,with quality care,to make it an important part of community health care.

Objective To investigate the effect of transforming growth factor-?1 (TGF-?1) on cell cycle,cell proliferation,and invasive capacity of human colon cancer cells of the line SW480,for its role in colon carcinogenesis and development,as well as its application in gene therapy for colon cancer with RNA interference.Methods The recombinant expressing plasmids pEGFP-N1-TGF-?1 was constructed and transfected into SW480 cells by Lipofectamine 2000.The expression of TGF-?1 mRNA and protein in the transfected SW480 cells were detected by fluorescent microscopy,RT-PCR and Western blot analysis respectively.The cell proliferation of SW480 cells was tested by MTT assay,the cell cycle was analyzed by flow cytometry,and the invasion ability of SW480 cells were investigated by Transwell-matrigel invasion chambers.Results After transfected into SW480 cells,both the mRNA and protein levels of TGF-?1 were effectively increased (P