Objective To investigate the effect of CDA-Ⅱ on t he expression of cyclin D1 of breast cancer cells. Methods The br east carcinoma cell lines MCF-7 and MDA-MB-231 were cultured in vitro tog ether with CDA-Ⅱ, with retinoic acid as positive control, and the effect of CD A-Ⅱ on the growth curve as well as the expression of cyclin D1 of breast carci noma cell lines was observed. Results CDA-Ⅱ may reduce the gr owth of MCF-7 and MDA-MB-231, cripple their proliferative ability and inhibit the cyclin D1 expression of breast cancer cell lines. Conclusion CDA-Ⅱ may inhibit the growth and proliferation of MCF-7 and MDA-MB-231, reduce the expression of breast carcinoma cell cyclin D1, hence showed a good a pplied prospect for the treatment of breast carcinoma.

Objective To analyze factors influencing β-blocker response rates of patients with portal hypertension and to explore the effect of nursing interference for increasing response rates with goal-directed nursing model. Methods 83 cases of portal hypertension were enrolled.Questionnaire about medical visiting behaviors and medication compliance were compared and analyzed after routine medication for 3 months.Bivariate and multivariate regression analysis were performed and related factors were used to establish a goal-directed nursing model for nursing interference guidance.52 non-response patients were randomly divided into the study group(27 cases)and the control group(25 cases).In the study group,compensation system and supportive-education according to Orem model were given,while the control group was given routine nursirng.Response rates and related factors were compared after 3 months of nursing intervention. Results Awareness of self-care responsibility,knowledge about the medication target,risk of sudden drug withdrawal,related medical knowledge,ability for pulse rate measurement and dose adjustment according to pulse rate changes were related to the response rates.Multivariate regression analysis showed that awareness of self-care responsibility,sudden drug withdrawal,utilization of social support and regular return visit were the independent factors.In the study group,ability for pulse rate measurement and dose adjustment acconding to pulse rate changes improved,phenomenon of sudden drug withdrawal declined and response rate increased,comparing with the control grpup. Conclusins For patients fail to respond to β-blockers,compliance behavior and medication compliance should be analyzed,and much attention should be paid to the education of the related medical knowledge,risk of sudden drug withdrawal and drag using targets.Goal-directed medel could be a guidance for nursing intervention to increase the response rates.

Objective To improve the diagnosis and treatment of liver trauma. Methods We retrospectively analyzed different therapeutic means on liver trauma including 79 cases with operative treatment and 27 cases with nonoperative treatment. Results In nonoperative treatment group, 25 cases were cured, 2 cases died. In operative treatment group, 73 cases were cured, 6 cases died. Cure rate was 92.5%(98/106), mortality was 7.5%(8/106). 5 cases died of major blood vessels rupture, 3 cases died on multiple organ function failure. Postoperative complications included 3 cases of subphrenic infection, 10 cases of hepatic abscess, 8 cases of pleural hydrops, 7 cases of incisional infection. Conclutions Type I of liver trauma can be treated by nonoperation, type Ⅱ～Ⅳ of blunt liver trauma can be treated by nonoperation on the condition of intensive monitoring. Type Ⅱ～ Ⅵ of liver trauma should be operated on emergently in case of massive intraabdominal bleeding and combined organ injury.

Objective To construct a rat fixation device for local infra-red irradiation in rats, observe the binding effect of this fixation device, and assess its practical application.Methods Twelve SD rats were held by this home-made simple device.The holding time was recorded at room temperature (24℃ to 26℃), 38℃ to 39℃ and 42℃ to 43℃ by infrared irradiation, respectively, and the maximum observation point was 60 min.Results Most rats (10/12) were held for more than 30 minutes at room temperature, 38℃ to 39℃ and 42℃ to 43℃ infrared irradiation.While 8 rats reached 60 min.There was no statistically significant difference among the holding times at various temperatures (P > 0.05).Conclusion This self-made device is simple, easy to operate and can be used to hold rats for a long time, and is a convenient and reliable holding device in animal experiments.

China ; Humans ; Immunohistochemistry ; standards ; Pathology, Clinical ; methods ; Quality Control

Objective To study the population's iodine nutritional status of Yanping area of Nanping City,Fujian Province,and to provide scientific basis for prevention and control of iodine deficiency disorders (IDD).Methods According to 2014 Key Population Iodine Nutrition in Fujian Province and the Related Health Monitoring Programme,five subareas were selected according to the five directions as east,west,south,north and centre in Yanping area,and one township was selected in each subarea.In addition,one subdistrict administrative office was chosen in urban area,and one neighborhood committee was selected as a monitoring site.Water iodine detection:two water samples from centralized water supply pipe were collected in selected townships and subdistrict administrative office,and arsenic cerium catalytic spectrophotometry was used to detect the level of water iodine.Salt iodine detection:four administrative villages were selected in each monitoring township,15 households were selected in each village,and a total of 300 households were selected;and 31 households were selected in urban area.The General Test Method for Determination of Iodine in Salt Industry (GB/T 13025.7-1999) was used to detect the level of edible salt iodine.Urinary iodine detection:①children aged 8-10 years old:one primary school was chosen in each monitoring township,30 children aged 8 to 10 were selected in each primary school,and a total of 150 children (half males and half females) were selected;one primary school was chosen in monitoring urban area,and 100 children (half males and half females) aged 8 to 10 were selected;②pregnant women and nursing mothers:20 pregnant women in each monitoring village were selected,and a total of 100 pregnant women were selected;50 pregnant women and 50 nursing mothers were selected in urban area;③adults aged 18 to 45 years old:50 adults (half males and half females) were selected in urban area.Urine samples at random times during the day were collected,and Arsenic Cerium Catalytic Spectrophotometry for Test of Urinary Iodine (WS/T 107-2006) was used to detect the level of urinary iodine.Goiter examination of children aged 8 to 10:palpation was used to examine goiter of all children.Results A total of 12 drinking water samples were collected,the levels of water iodine were all ＜ 3.5 μg/L.A total of 331 edible salt samples were collected,the median of salt iodine was 23.3 mg/kg,iodized salt coverage rate was 100.0％ (331/331),and qualified iodized salt consumption rate was 93.1％ (308/331).A total of 250 children were examined by the thyroid palpation,the rate of goiter was 0.8％ (2/250);the median of urinary iodine was 174.0 μg/L;the medians of children in rural and urban areas were 192.3 and 155.7 μg/L,respectively,the difference was statistically significant (Z =-3.582,P ＜ 0.01),and urinary iodine level of rural children was higher than that of urban children.Totally 150 pregnant women urine samples,50 nursing mothers urine samples,52 adults urine samples were collected,respectively,the medians were 143.7,116.2 and 115.6 μg/L,respectively,urinary iodine of pregnant women was lower than national standard (≥ 150 μg/L).Conclusions The population's iodine nutrition level is appropriate.At the same time,there are special people (pregnant women) at risk of iodine deficiency.

BACKGROUND: Tissue-engineered spinal cord has brought new treatment ideas for spinal cord repair.OBJECTIVE: To construct the tissue-engineered spinal cord by chitosan alginate scaffold and adipose-derived mesenchymal stem cells (ADMSCs), and to investigate its repairing effects on acute spinal cord injury in rats.METHODS: The spinal cord hemisection model was established in 48 Sprague-Dawley rats and then, rat models were randomly divided into four groups: model group, scaffold group, ADMSCs group and tissue-engineered spinal cord group,followed by direct suturing of the dura mater, implantation of chitosan alginate scaffold, implantation of ADMSCs, and implantation of tissue-engineered spinal cord, respectively. The limb motor function of rats was evaluated based on the Basso-Beattie-Bresnahan score at 1, 3, 7 weeks after transplantation. Immunofluorescence staining and hematoxylin-eosin staining of the spinal cord specimens were performed at 7 weeks after transplantation.RESULTS AND CONCLUSION: (1) Motor function of the hind limb: At 3 and 7 weeks after transplantation, the Basso-Beattie-Bresnahan score was highest in the tissue-engineered spinal cord followed, followed by the ADMSCs and scaffold groups, and lowest in the model group (P < 0.05). (2) Immunofluorescence and hematoxylin-eosin staining: in the model group, the spinal cord injury area was infiltrated with a large number of fibroblasts and inflammatory cells. In the scaffold group, there was no scar formation; neuron-specific enolase-positive cells, glial fibrillary acidic protein-positive cells and a few neurofilament protein 200-positive cells were found in the junctional area. In the ADMSCs group, the spinal cord injury area was filled with scar tissue, and a large number of glial fibrillary acidic protein-positive cells were found. In the tissue-engineered spinal cord group, there was no scar tissue, and there were a large number of neuron-specific enolase-positive cells, a small amount of CM-Dil-labeled ADMSCs and more glial fibrillary acidic protein-positive cells. Neurofilament protein 200 positive cells were connected to each other at the junction. These findings indicate that the tissue-engineered spinal cord constructed by chitosan alginate scaffold and ADMSCs can promote spinal cord repair after acute spinal cord injury.

Objective To explore the feasibility and clinical effects of rotary cutter in laparoscopic splenectomy in treatment of traumatic spleen rupture.Methods The study retrospectively identified 10 cases with traumatic spleen rupture treated with laparoscopic splenectomy from June 2014 to June 2016.Results Total laparoscopic splenectomy combined with rotary cutter was completed successfully in 9 cases and 1 case was converted to open laparotomy due to intraoperative uncontrollable hemorrhage. The former operative time was 95 ~ 170 min, the estimated intraoperative amount of blood loss was 300 ~ 800 ml and autologous blood transfusion of 400 ~ 1 200 ml was conducted. The postoperative hospital stay was 8 ~ 14 d. No serious complications were found in the cases followed-up for 3 ~ 24 months after operation.Conclusion Laparoscopic splenectomy combined with rotary cutter is not only feasible and safe but also has the merits of minimally invasive surgery. It can be applied in treating those with abdominal trauma and with benign lesions in spleen. So it deserved promotion and application in clinical work.

One hundred and ninety-one rats were injured with blast super-pressure and 64 out of the 191 died within 30 minutes after injury.The characteristics of the myocardial damages of the rats were as follows:(1)Disturbance of myocardial circulation;(2)Air embolism and interstitial emphysema;(3)Degeneration and necrosis of the myocardium; severance of myocardial fibers ;and separation of intercalated discs.It was speculated that the causes of these myocardial pathological changes are the direct damage of blast superpressure and the secondary effects of pulmonary damages.Protection of the heart and the lungs from blast super-pressure injury is the key to reduce the number of immediate death in blast injury.

Objective To evaluate the role of radiotherapy in the management of dermatofibrosarcoma protuberans. Methods Between 1995 and 1999, 18 patients with pathologically confirmed dermatofibrosarcoma protuberans were retrospectively analyzed. Nine patients were referred to our hospital for primary treatment and the remaining 9 patients came with recurrent disease. Fifteen of 18 patients received surgery followed by radiotherapy. Among them, 14 patients underwent complete resection and 1 partial resection. Majority of patients received 50～60 ?Gy. One patient received 37.5 ?Gy because he refrained from further treatment. Two patients received 52 ?Gy and 50 ?Gy due to unresectable extensive lesion. One of 18 patients was treated by total resection alone.Results The median of follow up time was 26 months with a range of 0.5～5 years. Sixteen patients (8.9%) are disease free. One patient developed local recurrence and one died of malignant progression. These two patients were treated with radiotherapy alone or surgery only. The local control rates were 100% (15/15) for patients who were treated with surgery and radiotherapy. Conclusions Postoperative radiotherapy is able to decrease the local recurrence rate in dermatofibrosarcoma protuberans.