Objective The article introduces the present status of the application of comparative proteomics in study of tumor marker. Methods This essay review the present status and advances of the application of comparative proteomics in study of tumor marker through refer considerable literatures about proteome, proteomics and tumor marker. Results Follow the study of human genome deepening; the paradox between the finiteness of genes’ number and stability of genes’ structure and the variety of the life phenomena is more conspicuous. Then, the study of proteomics was pushed to the advancing front of life science research. The application of comparative proteomics to tumor research becomes a hot spot nowadays. Conclusion Screening tumor marker via comparative proteomics is an extremely promising research.

Aim To study preparation of lutein from the extraction Marigold flowers by biotransformation.Methods Screen bacterium for lutein esters biotransforming by studying on the biotransformation of lutein esters by Penicillium on tabulate revulsive culture medium.Porule of and Aspergillus niger Penicillium could transform lutein esters into lutein,but biotransformation of Penicillium was better than Aspergillus niger.Porule of Pleurotus ostreatus could not grow on revulsive culture medium,but Pleurotus ostreatus could grow on the culture medium and transformed lutein esters into lutein.Research of shaking flash fermentation of Penicillium for trans-forming lutein esters.When pH is 6.0,quantity of incubation is 2 bacteria plugs and fermentation time is 12～24 hours,the transformation is completed and the content of lutein is 91.6%(HPLC)。Result Prolonging the transformation time will improve the percent of lutein but the content of lutein decreased.Conclusion Biological transrormation can be used from calendula officinacis extract preparation.

ObjectiveTo study the hemodynamic changes in combined portoazygous devascularization and mesocaval shunt for treating portal hypertension and the effect of this operation. Methods100 cases of portal hypertension who received this combined operation from 1980 to 1995 were analyzed retrospectively. ResultsFree portal pressure(FPP)after shunt decreased to 1.92 kPa,which decreased portal pressure and preserved with (2.46 ± 0.30)kPa to maintain a good inflow to the liver and the encephalopathy rate was lowered. There was no death, and the effect was satisfactory. 5～15 years follow-up of 89 cases showed no rebleeding,with only 5% of encephalopathy and 15% of total mortality. Among them, 13 cases of Child C died of late-stage hepatopathy, such as liver failure, liver cancer and hepatorenal syndrome,with 94.5% of 5-year survival rate and 68.5% of 10-year survival rate. ConclusionThis combined operation is characterized by complete devascularization of sustaining varices and distal partial shunt with logical diameter. A perfect combination of both shunt and devascularization, it's one of the best choices currently available for treating portal hypertension in China.

Objective To evaluate the efleet of continuous subcutaneous insulin infusion (CSII)combined with mefformin hydroehloride( Met H)on type 2 diabetic patients. Methods 240 patients with type 2 diabetes melli-tus (T2DM) were divided into 3 groups randomly: CSII combined with Met H ( A group: n=80) ; simple CSII ( B group:n=80) ; multiple deliver insulin (MDI) ( C group: n=80). The levels of blood glucose, HbA1 e, therapeutic time, a-mount of insulin, Fins ,C-P, HOMA-IR and IAI were compared among them before and after 10d of treatment. Results GroupA, B eornpared with group C, the levels of HbA1 e significantly deeroased in other two groups [ ( 8.0±3.2)%vs. (6.9±1.4)% and(7.0±1.2)% ] (aU P<0.05),so did the therapeutic time[(6.0±1.9)d vs. (3.0±1.4) and(3.5±1.5) d] and insulin dose[ (55.0±17.0) U/d vs. (38.0±15.0) U/d and(45.0±16) U/d] (all P<0.05). The insulin dose was much less in group A than in group B. Versus group B and C,the HOMA-IR of group A (0.8±0.3 vs. 1.5±0.4 and 1.6±0.4)decreased(P<0.05) ,and IAI(-2.0±4.3 vs. -3.6±4. 2 and -4.1±4.3) increased (all P<0.05), without the function impairments of heart, liver or kidney. Conclusion CSII in combi-nation with mefformin hydreehloride can significantly enhance the treatment effect of the patients with type 2 diabetes mellitns; reduce blood glucose and HbA1 e, improve insulin resistenee,lessen insulin need.

Cartilage injury has a very limited capacity of recovery by itself due to the lack of blood vessel in cartilage tissues. Current studies indicate that many kinds of cytokines have the function of promoting cartilage formation or repairing the injured cartilage with cartilage-like tissues. Cell-mediated transfer of the respective genes may ideally combine the supply of a chondrogenic cell population with the production of certain factors se-creted from the lesion and to promote the repairing of the lesion, which is considered the best treatment. Gene therapy based on this technology has developed rapidly in recent years. This review aims to summarize some of the development of the research in the field.

IL-17,as a pro-inflammatory cytokine,is one of the early initiation factors of the inflammato-ry response induced by T cells.It can induce and regulate multiple immune responses.Recent studies have re-vealed that myeloid neutrophils,macrophage,mast cell and other innate immune cells all can secrete large a-mount of early responding IL-17 in organs suffering ischemia/hypoxia,and in turn can activate,amplify and re-cruit neutrophils to the reperfusion-damaged tissue to release large amount of free radicals and lysozyme that cause IRI.Researchers have also provided evidence that appropriate administration of anti-IL-17 mono-antibody to neutralize IL-17 during early reperfusion stage would reduce the tissue damage.The purpose of this review is to summarize the research progress of the effects of IL-17 produced by innate immunocyte on organ reperfusion injury.

Objective To explore the application probability of AFLP method (Amplified Fragment Length Polymorphism) for the genetic study of complicated samples and to make clear the analytical approach on polymorphic fragments.Methods An AFLP fragement sequence,resolved between murines with high and low metastatic hepatocarcinoma,was retrieved from a reference paper and was annotated on NCBI database by different BLAST programs. Results The retrieved information varied along with the different BLAST programs, nevertheless BLASTX program provided more comprehensive information and showed that the differentiated fragment exhibited high homology with terminase large subunits from Burkholderia phage Bups phil and Mycobacterium tuberculosis H37Ra. However, there is no detailed information for the latter,key words search returned the relationship between terminase and the formation and metastasis of tumors and provided the further research clues. Conclusions Whole genome screening in parallel of AFLP markers between genetically differentiated paired materials would readily produce informatic fragments. Further analysis of the sequenced fragments by informatic methods would facilitate the subsequent research.

Objective To investigate the clinical characterisitics of chronic panereatitis(CP) with mass.Methods The clinical features,radiologieal and pathologic records of 39 cases of chronic pancreatitis with mass confirmed pathologically between January 2005 to December 2007 were retrospectively reviewed,and compared with 17 surgical pathologically confirmed patients with pancreatic cancer.Results The jaundice was found in 14 of 39 patients with pancreatits and 1 of 17 patients with pancreatic cancer,with significant difference between two groups(χ2=0.111,P=0.045).Elevated serum CEA and CA19-9 were found in 0 and 12 patients with pancreatitis.respectively,and 3 and 11 patients with pancreatic cancer,respectively(P=0.025 and=0.018,respectively).CT examination showed that the atrophy pancreas and the infiltration of surrounding and blood vessels were found in 0 and 5 patients with pancreatitis or 3 and 8 patients with pancreatic cancer,respectively(all P values＜0.05).The magnetic resonance cholangiopanereatography (MRCP) examination revealed that dilation of bile and pancreatic ducts,and calculus in pancreas duct were found in 14,2 and 15 patients with pancreatitis,or 11,6 and 2 patients with pancreatic cancer,respectively(all P values ＜0.05).Pancreatic cancer was confiremed by endoscopic ultrasonography guided fine needle aspiration(EUS-FNA)in 10 of 14 patients with pancreatic cancer,but none in 18 patients with pancreatitis.Conclusions It is difficult to diagnose CP with mass,but it will helpful ifassists diagnosis with clinical features,tumor markers and image examinations,especially with biopsyexamination.

106 ethmoidal specimens ranging from 12 to 40 weeks of embryonic age were cut into serial sections and observed by histological method. The results showed that the ethmoid sinus began its development at about 21-23 weeks of fetal life. The frontal folds or furrows were formed by extensions or evaginations of the nasal mucous membrane from the superior and lateral wall of the middle and superior nasal meatuses. The ethmoid cells appeared at 24-26 weeks and they kept their own small ostia that opened into the middle and superior nasal meatuses. The number and size of the ethmoid cells were increased with the age. The cell cavities showed balanced enlargement. At birth each ethmoid labyrinth had 6-11 cells, measuring on the average 1.7?1.3?2.0 mm~3 in size. The distribution of the ethmoid cells approached those of mature age. The epithelium of the ethmoid sinus was cuboidal or low columnar in shape. Cilia appeared sparse. Tunica propria, mainly composed of the connective tissue and its stroma, was thick, loose, and less vascular and glandular. It suggested that the ethmoid sinus was one of the accessory nasal sinuse that developed first. The ethmoid sinus presented at birth and its appreance were not synchronous, but their location were relatively invariable. The histological structure of the mucous membrane in the ethmoid sinus was somewhat different from those of the nasal mucous membrane. It showed that the development of the ethmoid mucous membrane was still imperfect in the newborn.