0.05).Conclusion The therapeutic mechanism of AEP of Chinese herbal medicine for asthma may be related with the inhibition of EOS infiltration,the decrease of peripheral IL-4 content and the increase of IFN-?.

Objectives To study the incidence of Trichomonas vaginalis(TV)infection in Chinese male patients with nongonococcal urethritis(NGU),to evaluate the sensitivity and specificity of urine-based and urethral swab polymerase chain reaction(PCR)detection,and to set up a method of non-invasive detection for male TV infection.Methods One hundred and five male NGU patients were collected from the STD clinic.Two urethral swabs were obtained from each patient,one for InPouch TV culture system,the other for PCR;one urine specimen(first-void urine or first-catch urine)was also collected for PCR detection.Culture was considered as the"gold standard".Compared with the culture,the sensitivity,specificity,positive predictive value(PPV)and negative predictive value(NPV)of two PCR detection were calculated.Results The positive rate of InPouch TV system detection was4.76%(5/105);the positive rate of urine-based PCR and urethral swab PCR detection was3.81%(4/105)and4.76%(5/105),respectively.In comparison with the culture,the sensitivity,specificity,PPV and NPV were80%,100%,100%and99%for urine-based PCR and80%,99%,80%and99%for urethral swab PCR.Conclusions TV is one of the etiological agent for male NGU,the positive rate of TV is4.76%in our study.The urine-based PCR detection has a higher sensitivity and specificity and provides a noninvasive method which is feasible in practice.

Objective To investigate the relationship between serum 25-hydroxyvitamin D level and gestational diabetes mellitus (GDM).Methods The fasting plasma glucose,fasting insulin,25-hydroxyvitamin D,and high sensitive C-reactive protein (hs-CRP) levels in the normal glucose tolerance group and gestational diabetes mellitus (GDM) group were measured.The homostasis model assessment insulin resistant (HOMA-IR) and homostasis model assessment β cell (HOMA-B) were calculated for assessing the insulin resistance and β-cell function.The relationship between 25-hydmxyvitamin D and hs-CRP,HOMA-IR,and HOMA-B were analyzed.Results The level of 25-hydroxyvitamin D in the GDM group [(2.16 ± 0.38) nmol/L] was significantly lower than that in the normal glucose tolerance group [(4.13 ±0.79) nmol/L](P =0.001).Serum 25-hydroxyvitamin D level was negatively correlated with hs-CRP (r =-0.448,P =0.002) and HOMA-IR (r =0.396,P =0.004),but was positively related with HOMA-B (r =0.495,P =0.001).Conclusions The serum 25-hydmxyvitamin D level remarkably decreases in GDM patients.Vitamin D deficiency may cause the functional damage of beta cell of islet,increase insulin resistance,and thus contribute to the development of GDM.

Objective To investigate the isolation and antimicrobial resistance of hypermutable Pseudomonas aeruginosa strains.Methods The cultured bacteria were identified by API 20NE system.The susceptibilities of bacteria were detected by disk diffusion method and the results were confirmed with the rules of NCCLS/CLSI.The hypermutable strains were identified byhigh concentration of rifampin-resistant growth assay.Results Among analyzed 100 strains,37 strains(37.0%) were hypermutable.The resistant rates of hypermutable strains to imipenem,meropenem,cefoperazone/sulbactam,piperacillin/tazobactam,ceftazidime,cephfime,aztreonam,amikacin and ciprofloxacin were significantly higher than those of non-hypermutable strains.The multidrug resistance rates of hypermutable strains were also higher than those of non-hypermutable strains.Conclusion The hypermutable strains of Pseudomonas aeruginosa sourced from chronic respiratory tract infections dominantly.The hypermutable strains were hyper-resistant and multidrug resistant to many antibiotics,so strengthened monitoring should be encouraged to control the prevalence of hypermutable Pseudomonas aeruginosa strains.

Objective To study the population's iodine nutritional status of Yanping area of Nanping City,Fujian Province,and to provide scientific basis for prevention and control of iodine deficiency disorders (IDD).Methods According to 2014 Key Population Iodine Nutrition in Fujian Province and the Related Health Monitoring Programme,five subareas were selected according to the five directions as east,west,south,north and centre in Yanping area,and one township was selected in each subarea.In addition,one subdistrict administrative office was chosen in urban area,and one neighborhood committee was selected as a monitoring site.Water iodine detection:two water samples from centralized water supply pipe were collected in selected townships and subdistrict administrative office,and arsenic cerium catalytic spectrophotometry was used to detect the level of water iodine.Salt iodine detection:four administrative villages were selected in each monitoring township,15 households were selected in each village,and a total of 300 households were selected;and 31 households were selected in urban area.The General Test Method for Determination of Iodine in Salt Industry (GB/T 13025.7-1999) was used to detect the level of edible salt iodine.Urinary iodine detection:①children aged 8-10 years old:one primary school was chosen in each monitoring township,30 children aged 8 to 10 were selected in each primary school,and a total of 150 children (half males and half females) were selected;one primary school was chosen in monitoring urban area,and 100 children (half males and half females) aged 8 to 10 were selected;②pregnant women and nursing mothers:20 pregnant women in each monitoring village were selected,and a total of 100 pregnant women were selected;50 pregnant women and 50 nursing mothers were selected in urban area;③adults aged 18 to 45 years old:50 adults (half males and half females) were selected in urban area.Urine samples at random times during the day were collected,and Arsenic Cerium Catalytic Spectrophotometry for Test of Urinary Iodine (WS/T 107-2006) was used to detect the level of urinary iodine.Goiter examination of children aged 8 to 10:palpation was used to examine goiter of all children.Results A total of 12 drinking water samples were collected,the levels of water iodine were all ＜ 3.5 μg/L.A total of 331 edible salt samples were collected,the median of salt iodine was 23.3 mg/kg,iodized salt coverage rate was 100.0％ (331/331),and qualified iodized salt consumption rate was 93.1％ (308/331).A total of 250 children were examined by the thyroid palpation,the rate of goiter was 0.8％ (2/250);the median of urinary iodine was 174.0 μg/L;the medians of children in rural and urban areas were 192.3 and 155.7 μg/L,respectively,the difference was statistically significant (Z =-3.582,P ＜ 0.01),and urinary iodine level of rural children was higher than that of urban children.Totally 150 pregnant women urine samples,50 nursing mothers urine samples,52 adults urine samples were collected,respectively,the medians were 143.7,116.2 and 115.6 μg/L,respectively,urinary iodine of pregnant women was lower than national standard (≥ 150 μg/L).Conclusions The population's iodine nutrition level is appropriate.At the same time,there are special people (pregnant women) at risk of iodine deficiency.

Objective To establish the method of content determination of Piperine in Biba Zhitong Tincture.Methods HPLC was adopted in determining the content of piperine in Biba Zhitong Tincture.C18 chromatographic column was used with the column temperature being the room temperature.The mobile phase was tetrahydrofuran-methanol-water(V∶V∶V=30∶30∶50)with the velocity of 1.0 mL/min.The detection wavelength was 343nm,and the sampling volume was 10?L.External standard method was chosen for content calculation.Results The inner peak area of piperine(8.3～41.5 mg/L)showed fine linear relation with mass concentration of control group.Its regression equation was A=3413186?-7467.7,r= 0.999 8,with the average recovery ration being 99.7%,and RSD=1.8%(n=6).Conclusion The method is simple,convenient,good reproducibility and non-interference,and can effectively control the quality of the preparation.

We tried to clarify the effect of traditional Chinese medicine in the cases of diabetic retinopathy. The 12 male patients and 21 female patients of diabetic retinopathy are administered Kampo extracts.
Most of the patients were classified as a simple type or a preproliferative type. These patients were divided into three types of pathological conditions diagnosed by Traditional Chinese Medicine such as “deficiency of Qi” “Yin-deficiency” and “deficiency of Oi and Yin” accompanied stagnation of pathological blood.
After the administration of Kampo extract for over 6 months, the effects were recognized in more than 80% of the patients from the observation on absorption of hemorrhage and white flecks of the retina and the improvement of visual acuity.
The basic pathological conditions should be considered for prescription of formulations for diabetic retinopathy.

Objective To investigate the relationship between the type of gliding contusion and its mechanism with site of force.Methods The site undergoing contusion and ways of force are respectively determined by skull anatomy location and details of these cases.Then,the 132 specimens of brain,which have been fixed by formalin,are sliced in coronal section and sagittal section and stained with HE,observed under microscope.ResultsGliding brain contusion could appeared at the top and bottom region of brain respectively.Top-injury type,were observed in 65 cases(49.24%),base-injury type,were found in 38 cases(28.78%).There were 29 cases(21.96%) in which contusion could be found at both top and base of brain,we called mixed type.We found that the injury area depend on the ways of force-act:the top-injury type mostly caused by the force on cupular part of pars zygomatica in acceleration,the base-injury type mainly caused by the force on occipitalia in deceleration and the mixed type caused by the force on the boundry of the calvaria and occipital in deceleration.Conclusion The type of gliding contusion is relevant with mechanism and site of force.

The extensive application of radiotherapy and the long survival of patients with malignant tumors have led to an in-creased incidence rate of radiation-induced osteosarcoma (RIOS), one of the most critical radiation-induced complications. Compared with primary osteosarcoma, RIOS has higher grade and poorer prognosis, which reduces the survival of patients with cancers. However, RIOS has not been fully understood in the clinical setting. This paper summarizes the latest progress at home and abroad, focusing on the incidence rate, risk factor, latency, and diagnostic criteria of RIOS. The molecular mechanism of RIOS is associated with the muta-tion of p53, the activation of the Wnt/β-catenin signaling pathway, and the superposition of multiple alleles. Furthermore, we discuss the differences in clinical characteristics, imaging findings, treatment, and prognosis between primary osteosarcoma and RIOS.

12E7 Antigen ; Adolescent ; Alkaline Phosphatase ; metabolism ; Antigens, CD ; metabolism ; Cell Adhesion Molecules ; metabolism ; Diagnosis, Differential ; Female ; Follow-Up Studies ; Gonadoblastoma ; pathology ; Humans ; Immunohistochemistry ; Isoenzymes ; metabolism ; Neoplasms, Germ Cell and Embryonal ; metabolism ; pathology ; surgery ; Ovarian Neoplasms ; metabolism ; pathology ; surgery ; Proliferating Cell Nuclear Antigen ; metabolism ; Sex Cord-Gonadal Stromal Tumors ; metabolism ; pathology ; surgery