Humans ; Thyroid Neoplasms ; pathology ; therapy

Objective To summarize the method and experience of pharmaceutical care for a patient with insulin resistant. Methods The rationality of the treatment plan was analyzed by the clinical pharmacist, and the entire pharmaceutical care process was implemented to the patient. Results Doctors took the advice of clinical pharmacists, perfected the treatment plan. The treatment was safe and effective. The patient was discharged with a better health condition. Conclusion Clinical pharmacist integrates in the treatment team,and to promote the rational use of drugs.

Leber congenital amaurosis (LCA) is extremely severe hereditary retinal dystrophy,because it leads to congenital bilateral blindness in early childhood.With the development of molecular genetics and the therapeutic gene replacement technology,gene therapy clinical trials have obtained exciting results on the basis of relatively satisfied with preliminary clinical experimental results by adeno-associated virus (AAV) vector-mediated gene therapy in the past decade.These researching methods include intravitreal injection and subretinal space injection of gene vector,and the investigating indexes include evaluations of visual function and safety,such as immune reaction of the animals,ocular histopathological change,complications and bio-distribution of gene sequence.The preliminary success of the LCAⅡ Ⅱ gene therapy will give some clues to the other inherited retinal diseases.This review focuses on the present status of pre-clinical animal experiments of its gene therapy.

Objective To investigate the risk factors in the outcome of neonatal pulmonary hemorrhage. Methods A total of 69 cases of neonatal pulmonary hemorrhage from January 2005 to December 2011 were studied. They were divided into 2 groups according to clinical outcome (death or alive). The data of the two groups were compared using single factor analysis. The risk factors were analyzed using multi-factor analysis. Results The death of neonates with pulmonary hemorrhage was correlated with aspiration pneumonia, coagulation abnormalities, DIC, heart failure and MPV. Multi-factor analysis showed that DIC (OR=6.90, 95%CI:1.514-31.419), heart failure (OR=9.62, 95%CI:1.710-54.150) and MPV<11 prior to pulmonary hemorrhage (OR=7.01, 95%CI:1.475-33.312) were the independent risk factors of neonatal pulmonary hemorrhage. Conclusions For the neonatal pulmonary hemorrhage with DIC, heart failure and low MPV, active intervention should be implemented.

Objective To establish a liquid chromatographic-mass spectrometric method ( LC-MS/MS method ) for determination of dextrorphan in human liver microsome. Methods LC-MS/MS was adopted with carbamazepine serving as an internal standard.The separation was performed on Agilent ZORBAX XDB-C18 column (2.1 mm×50 mm, 3.5μm), with mobile phase consisting of 0. 05% formic acid methanol-0. 05% formic acid in gradient elution. Dextrorphan and carbamazepine were detected on multiple reaction monitoring(MRM)mode by transitions from precursor to production(m/z 258.1→199.1, 237.1→194.1). Results The linear range of dextrorphan concentration was 19.22-768 960 ng.L-1(r=0.999 8), and the lowest quantification limit was 19.22 ng.L-1.The relative recoveries were 94.02%-98.74%, and the RSDs of intra-day and inter-day were within 10%.IC50 of psoralen on CYP2D6 was 0.6μmol.L-1. Conclusion The LC-MS/MS method is proved to be rapid, sensitive and reproducible, psoralen is a strong inhibitor of CYP2D6.

AIM: To study the expression level of gene SNC66 in gastric cancer tissues, and investigate the relationship between the expression of SNC66 and the pathogenesis of gastric cancer.METHODS: Paired normal and cancerous paraffin-embeded tissues from 20 gastric cancer patients were tested by in situ hybridization (ISH) and in situ reverse transcription and polymerase chain reaction (ISRT-PCR)using ?-actin as a control.RESULTS: ISH and ISRT-PCR both showed that SNC66 were all strong positive expressed in 20 normal gastric mucosa.But in 20 gastric cancer tissues,the ISH showed that 8 were negative,8 were weak positive and 4 were strong positive. The ISRT-PCR showed that 6 were negative,9 were weak positive and 5 were strong positive when the PCR cycled 10 times;4 were negative and 16 were strong positive when the PCR cycled 25 times. CONCLUSION: There was significant expression reduction or defect of SNC66 in gastric cancer tissues.SNC66 should be further investigated as a gastric cancer negative relative gene.

OBJECTIVE:To establish the content determination method of Icariin in Fucongxiang liquid by HPLC.METHODS:HPLC was performed to determine Icariin on VP-ODS column.The mobile phase was acetonitrile:water(30∶70).Detecting wavelength was270nm.RESULTS:The detected concentration of Icariin was in the good linear range of2.8～28.0?g/ml(r=1.0000),and the average recovery was100.03%(RSD=0.54%,n=5).CONCLUSIONS:The estab-lished method is simple,sensitive,accurate and available for the quality control of Fucongxiang liquid.

Carotid stenosis is an important modifiable risk factor in ischemic stroke.The com prehensive diagnosis of carotid stenosis,including the comprehensive understanding of stenosis degree,plaque stability,and head and neck vessels,which has important guiding significance for the prevention and treatment of ischemic stroke.This article reviews the diagnostic values of digital subtraction angiography,sonography,magnetic resonance angiography and CT angiorgaphy in carotid stenosis.

Stimuli-sensitive drug delivery system (SSDDS) is an novel drug delivery carrier.It is sensitive to either the internal physiopathologic changes (pH,temperature) of the body or external stimulus signal (ultrasound,magnetic signal) and controls the release of the drugs that it carries according to the variation of physicochemical property which stimulated by the signals.SSDDS can be prepared from hydrogels,liposomes and magnetic nanoparticles.In contrast to non-stimuli-responsive drug delivery system,SSDDS has remarkable advantages including feedback regulation,stronger controllability and targeting therapy.This paper will review the advancement in stimuli-responsive drug delivery system in recent years.